GibberellicAcid(GA)isaverypotentplanthormone,whichregulates thegrowthrateofplantspromotingcellelongation.GibberellicacidstimulatesthecellsofgerminatingseedstoproducemRNAmoleculesthatcodeforhydrolyticenzymes.ItwasfirstdiscoveredinJapan,in1935asaresultofthestudyofaconditioncommoninriceplantscalled"foolishseedling"disease,whichcausedtheplantstogrowmuchtallerthannormal.

BSA-conjugatedgibberellicacid(gibberellinA3)

storelyophilized/reconstitutedat-20°C;thisaliquotecanbefreezedandthawedforuptofivetimesandshowedstABIlityforatleast2years.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

AS06193|anti-indoleaceticacidrabbitantibody

AS06195|anti-abscisicacidrabbitantibody

AS010933|GA3-BSA|gibberellicacidBSAconjugate

collectionofantibodiestoplanthormones

secondaryantibodiestoratIgG

Secondaryantibodies

TotalIgGconcentrationis20mg/ml.AntibodyhaslowaffinitytofreeGAandotherGAforms:1,4,5,7,9,53astestedbycompetitiveELISA.

UsingaconjugatedGibberellicacid3-Ethyl-Chloroformiate-(PC),antibodyspecificitywasperformedwithanELISAtestbycompetitionexperimentswiththefollowingcompounds:
CompoundCross-reactivityratio(a)Gibberellicacid3-ECF-BSA(GA3-BSA)1Gibberellicacid1-ECF-BSA(GA1-BSA)1/>50000Gibberellicacid4-ECF-BSA(GA4-BSA)1/>50000Gibberellicacid5-ECF-BSA(GA5-BSA)1/>50000Gibberellicacid7-ECF-BSA(GA7-BSA)1/>50000Gibberellicacid9-ECF-BSA(GA9-BSA)1/>50000Gibberellicacid53-ECF-BSA(GA53-BSA)1/>50000(a):Gibberellicacid3-ECF-BSAconcentration/Otherconjugatedacidsconcentrationathalfdisplacement;ECF=Ethyl-Chloroformiate,BSA:BovineSerumAlbumin.

1:100(IL)

dicotsandmonocots

noconfirmedexceptionsfrompredictedreactivityknowninthemoment

fordetailedimmunolocalizationprotocolcheckhere
totalIgGwaspurifiedbyammoniumsulfateprecipitation