| | product information | | Background | | Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. RPN6 associates with an ATPase subunit of the 19S proteasome regulatory complex, AtS6A. Alternative names: 26S proteasome non-ATPase regulatory subunit 11 homolog, 19S proteosome subunit 9, AtS9 26S proteasome regulatory subunit RPN6, AtRPN6, 26S proteasome regulatory subunit S9 homolog. | | Immunogen | | KLH-conjugated synthetic peptide derived from known sequences of RPN6 including Arabidopsis thaliana RPN6 sequence, UniProt: Q9LP45, TAIR: AT1G29150 | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified serum in PBS pH 7.4 | | Format | | Lyophilized | | Quantity | | 50 µg | | Reconstitution | | For reconstitution add 50 µl of sterile water. | | Storage | | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | Tested applications | | western blot (WB) | | Related products | | AS15 2832 | anti-RPN6 | 26S proteasome non-ATPase regulatory subunit 9 (other species), rabbit antibodies AS08 308 | anti-SUMO1 | Small ubiquitin-like modifier protein 1, rabbit antibodies AS08 349 | anti-SUMO3 | Small ubiquitin-like modifier protein 3, rabbit antibodies AS08 307 | anti-UBQ11 | Ubiquitin, rabbit antibodies AS08 307A | anti-UBQ11 | Ubiquitin, affinity purified rabbit antibody AS10 307S | UBQ11 | Ubiquitin protein (positive control) AS01 019 | anti-S5a | human 26S proteasome subunit, rabbit antibodyAS01 012 | anti-DS5a | Drosophila 26S proteasome subunit Rpn10, rabbit antibody Secondary antibodies | | Additional information | | | |
| application information |
| Recommended dilution | | 1:1000 (WB) |
| Expected | apparent MW | | 40 kDa |
| Confirmed reactivity | | Arabidopsis thaliana |
| Predicted reactivity | | Glycine soja , Gossypium arboreum, Medicago truncatula, Ornithogalum saundersiae, Oryza sativa, Populus trichocarpa, Ricinus communis, Solanum chacoense, Theobroma caco, Triticum urartu, Zea mays, Zostera marina |
| Not reactive in | | |
| Additional information | | |
| Selected references | | To be added when available, antibody released in May 2016. |
application example
![\"western](\"https://www.ebiomall.com/images/agrisera/201709/AS15_2845.jpg\")
15 µg or 10 µg or 5 µg of protein total extract from Arabidopsis thaliana are denatured at 70°C for 10 min then separated on 10% SDS-PAGE. Blotted 1 h 100V to Nitrocellulose membrane (GE Healthcare; 0.2 µm) tank transfert (Tris 50 mM; Glycine 40 mM; SDS 0.0375 % (m/v); methanol 20 % (v/v)). Blocked with TBS-T 0.1% powder milk 3% 1 h at room temperature. Blot α-RPN6 (AS15 2832A) was incubated 1 h at room temperature 1:1000 in TBS-T powder milk 3% and washed 2 times for 10 min with TBS T 0.1% at room temperature with agitation. Blot was incubated in secondary antibody anti-Rabbit IgG horse radish peroxidase conjugated diluted to 1:20 000 in TBS-T 0.1% from
Agrisera (AS09 602) 1 h at room temperature and washed 3 times for 10 min with TBST 0.1%. Development with 20x LumiGLO ® Reagent and 20x Peroxide (#7003 cell signaling). Exposure time vary between 8 to 30 min.
Courtesy of Dr. Claire Rosnoblet (MCF), Agroécologie Agrosup Dijon / INRA / Université de Bourgogne, France
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