|
| product information |
| Background | | Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays. |
| Immunogen | | Purified Rabbit IgG |
| Host | | Chicken |
| Clonality | | Polyclonal |
| Clone | | |
| Purity | | Affinity purified Chicken IgY |
| Format | | Lyophilized |
| Quantity | | 1 mg |
| Reconstitution | | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily. |
| Storage | | Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Tested applications | | ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC) |
| Related products | | AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugated |
| Additional information | | HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase |
