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product information |
Background | | Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. |
Immunogen | | Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992): 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco. |
Host | | Rabbit |
Clonality | | Polyclonal |
Clone | | |
Purity | | Total IgG |
Format | | Lyophilized in PBS pH 7.4 |
Quantity | | 2 mg |
Reconstitution | | For reconstitution add 100 µl of sterile water. |
Storage | | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
Tested applications | | Co-immunoprecipitation (Co-IP), Immunolocalization (IL), Western Blot (WB) |
Related products | | AS10 687 | PR-1 | Pathogenesis-related protein 1, rabbit antibodies AS12 2366 | PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thalianaAS07 208 | PR-2 | GLU I | class I beta-1,3-glucanase, rabbit antibodies for other species, not Arabidopsis thalianaAS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodiesAS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodies collection of antibodies to other proteins involved in a response to pathogen attack Secondary antibodies |
Additional information | | Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa) |