Plant custom made Western blot-1 is your choice of any 10 plant tissues of panel# 1. Total protein will be extracted from the selected tissues and subjected to rigorous quality control procedures before fractionation through large SDS-PAGE.  Blots can be used to:

• Quickly assess specific protein expression across different plant tissues.
• Determine size and relative abundance of protein  in different plant tissues.

Quality control:Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.

• Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
• The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
• The integrity of the blotted protein is tested by immunostaining of b-actin.

Description:The total protein samples  (75ug each ) are freshly extracted from plant leaves, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4^oC in a sealed bag with descant.

>>> 更多资讯详情请访问蚂蚁淘商城