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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
MouseC57custommadeWesternblot-2isyourchoiceofany10C57BL/6 mousetissueslistedinPanel#1to6.Totalproteinwillbeextractedfromtheselected10tissuesandsubjectedtorigorousqualitycontrolprocedures beforefractionationthroughlargeSDSgels.
Blotscanbeusedto:
- Quicklyassessspecificproteinexpressionacrossdifferenttissues.
- Determinesizeandrelativeabundanceofproteinindifferenttissues.
Qualitycontrol:
Everystepofpreparationofblots,fromharvestingtissuesandextractionofproteintoblottingiscarefullymonitoredtoensurethesuperiorqualityandperformance.
- Blotsaremadefromhighqualitytissuelysatesthatishomogenizedinaproteinlysisbuffercontainingcocktailof7mammalianproteaseinhibitors.Thequalityofproteinasindicatedbytheabsenceofsmear(nodegradation)andsharpnessandresolutionofproteinbandsareverifiedbydenaturedSDS-PAGEwithCoomassiebluestaining.
- TheefficiencyoftransferisroutinelycheckedbystainingproteinonmembraneswithreversIBLePonceaustaining.
- Theintegrityoftheblottedproteinistestedbyimmunostainingof b-actin.
Description:
Thetotalprotein samples(75ugeach) arefreshlyextractedfrom tissues,boiledinSDSsamplebuffer,fractionatedthough12.5%SDS-denaturinglargepolyacralamidegeleletrophoresis(SDSPAGE), andblottedontoPVDFmembranes.Eachblotisshippedinasealedbagatroomtemperatureandisgoodforoneyearifproperlystoreddryat4oCinasealedbagwithdescant.


