ORGANOTYPICLIMBCULTURES
-embryosaredissectedfromtimed-pregnantmicefrom10.5-11.5d.p.c.-limbbudsaremicrodissectedandplacedinholdingmedium(L15mediumsupplementedwith1xMITO+serumextender)(allmediaissupplementedwith100Upenicillin/mland0.1mgstreptomycin/ml)-limbsareallowedtositinholdingmediumanywherefrom20minto2hours-0.2mlofserum-freeexplantmedia(DMEM-F12mediumsupplementedwith5xMITO+withorwithout10%fetalbovineserum)isaddedtoeachwellofasix-welltissuecultureplatecontainingCyclopore(polyethyleneterphthalate;FalconLabware)inserts-limbsaretransferredfromtheholdingmediaontothesurfaceoftheCycloporemembranes(fortransfer,theendofa200祃Pipettetipisremovedwithasterilerazorblade(toenlargethehole),andlimbsaretakenupin5–10祃ofholdingmedia)-limbsaregrownonthetissuecultureinsertsina37oCincubatorunderanatmosphereof5%CO2-explantmediaisreplacedevery48hoursLimbscanbesuccessfullygrownfor3–4days
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