品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com

ORGANOTYPIC LIMB CULTURES

作者: 时间:2024-12-25 点击量:

ORGANOTYPICLIMBCULTURES


  • -embryosaredissectedfromtimed-pregnantmicefrom10.5-11.5d.p.c.
  • -limbbudsaremicrodissectedandplacedinholdingmedium(L15mediumsupplementedwith1xMITO+serumextender)(allmediaissupplementedwith100Upenicillin/mland0.1mgstreptomycin/ml)
  • -limbsareallowedtositinholdingmediumanywherefrom20minto2hours
  • -0.2mlofserum-freeexplantmedia(DMEM-F12mediumsupplementedwith5xMITO+withorwithout10%fetalbovineserum)isaddedtoeachwellofasix-welltissuecultureplatecontainingCyclopore(polyethyleneterphthalate;FalconLabware)inserts
  • -limbsaretransferredfromtheholdingmediaontothesurfaceoftheCycloporemembranes(fortransfer,theendofa200祃Pipettetipisremovedwithasterilerazorblade(toenlargethehole),andlimbsaretakenupin5–10祃ofholdingmedia)
  • -limbsaregrownonthetissuecultureinsertsina37oCincubatorunderanatmosphereof5%CO2
  • -explantmediaisreplacedevery48hours
  • Limbscanbesuccessfullygrownfor3–4days

    >>> 更多资讯详情请访问蚂蚁淘商城

    Plant media商品列表
    图片/货号 产品名/品牌 价格/货期 操作