Name

Elite^TM Protease Activity Assay Kit (Red Fluorescence)

Description

The kit uses a red fluorescent casein conjugate that is proven to be a generic substrate for a broad spectrum of proteases (e.g. trypsin, chymotrypsin, thermolysin, proteinase K, protease XIV, and elastase). In the intact substrate, casein is heavily labeled with a fluorescent dye, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly fluorescent dye-labeled short peptides.

Application

The assay is ideal for routine protease assays for the isolation of proteases, or for detecting proteases contamination in samples.

Size

500 assays

Detection

Microplate reader (Ex/Em = 540/590 nm)