CA-N405 Name Description TheElite™FluorimetricNADPHAssayKitprovidesasensitive,one-stepassaytodetectaslittleas100picomolesofNADPHina100μlassayvolume(1μM).IthasverylowbackgroundsinceitisrunintheredvisIBLerangethatsignificantlyreducestheinterferenceresultedfromBIOLOGicalsamples. Application Theassaycanbeconvenientlyperformedina96-wellor384-wellmicrotiter-plateformat.ItssignalcanbeeasilyreadbyeitherafluorescencemicroplatereaderatEx/Em=540/590nmoranabsorbancemicroplatereaderat~576nm. ThiskithasbeenusedforscreeningenzymeactivitiesthatuseNADP/NADPHasacofactor.IthasalsobeenusedforthesensitivedetectionofNADPHincell-basedassays.Comparedwiththeothercommercialkits,thisassayhashighersignal/backgroundratio. Size 400assaysin96-wellplates Ex/Em 540/590nm Detection Fluorescencemicroplatereader | ||||||||||||||
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