3DCellUSPongeismadeofhydroxypropylcellulose,aknownbiocompatIBLematerial,asthebasicmaterialthatisside-chainmodifiedforcrosslinkingandligandconjugation.



Thespongedisksarepre-sterilizedandreadyforcellseeding.Thecellseedingiseasy.Itcouldbeperformedthroughabsorptionofcellsuspensionwithdesiredcelldensityontothesurfaceofthedrysponge.After30-60minutesofincubation,freshpre-warmedculturemediumcouldbeaddedforextendedcellculturemaintenance.

TheCelluspongesareavailablein3forms:original,galactosylated,conjugatedwithcollagen.Manyresearchershaveusedtheseproducts,andtheyhavesuccessfullydemonstratedspheroidformationusingmanycelltypes.
 -Celluspongeisbestforcellsrequiringnospecificligandandvariouscancercelllines(i.e.MCF-7&MDA-MB,NIH3T3,humanforeskinfibroblastHFF);
 -Cellusponge-Galisbestforhepatocytesusedininvitrodrugtestinganddrugdevelopment(i.e.primaryrathepatocytes,primaryhumanhepatocytes,hepatocytecelllines);
 -Cellusponge-Collisbestforcellsrequiringcollagensubstratum(i.e.mouseandhumanembryonicstemcellsmESC&hESC,humanmesenchymalstemcells,primarycells).

Features:
1.Hydrogel-basedmacroporoussponge(exclusivepatentlicense).
2.Simpleandefficientcellseedingprocedure.
3.Cellsdistributedwellinthespongeporesandcouldgrowwell.
4.Formfunctionalspheroidswithin24hourspostseedingandremianstableforatleast40days.
5.Appropriatespheroidsizethatpreventsapoptosisinthespheroidscenter.
6.Compatiblewithwelledplatesandinserts.

Advantages:
1.Naturalsource
2.Spheroidsizeiscontrollableandunder200um.
3.Seedingisthesameasin2Dcellcultureanddoesnotrequireextrasteps.
4.Cellsgrowrapidlywithproperfunctions.
5.Compatibletoautomatedequipment.

Applications:
1.Generalsofttissue3Dculture
2.Spongeforinvivostudy
3.Stemcelldifferentiationplatforms
4.3Dprimarycellculturemodel
5.Drug/anti-viraltesting
6.Suitableforcryopreservation

ProductFormandPackageInformation:

Cellusponge	Product(Thickness:1mm)	Catalog#
9-mm/24disks	Cellusponge	S92424
(24-wellplate)	Cellusponge-Galactose	G92424
	Cellusponge-Collagen	C92424
6-mm/48disks	Cellusponge	S64848
(48-wellplate)	Cellusponge-Galactose	G64848
	Cellusponge-Collagen	C64848
9-mm/8disks	Cellusponge-Galactose	G92408
(24-wellplate)	Cellusponge-Collagen	C92408
6-mm/16disks	Cellusponge-Galactose	G64816
(48-wellplate)	Cellusponge-Collagen	C64816

3DCultureReferences:

1.Preparationofthree-dimensionalinterconnectedmacroporouscellulosichydrogelsforsofttissueengineering.
2.Arobusthigh-throughputsandwichcell-baseddrugscreeningplatform.
3.Purpose-drivenBiomaterialsresearchinliver-tissueengineering.
4.Galactosylatedcellulosicspongeformulti-welldrugsafetytesting.
5.Tetheredspheroidsasaninvitrohepatocytemodelfordrugsafetyscreening.
6.Morphologyandorganizationoftissuecellsin3Dmicroenvironmentofmonodispersefoamscaffolds.
7.Electrotaxisoflungcancercellsinorderedthree-dimensionalscaffolds.

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