HumanbasicFibroblastGrowthFactor(bFGF)isasingle-chainpolypeptidegrowthfactorthatplaysasignificantroleintheprocessofwoundhealingandisapotentinducerofangiogenesis.bFGFhasbeenshowntosupportthemaintenanceofundifferentiatedhumanembryonicstemcells.Italsostimulatestheproliferationofallcellsofmesodermalorigin,andmanycellsofneuroectodermal,ectodermalandendodermalorigin.bFGFisachemotacticandmitogenicagentforendothelialcellsinvitroandinducesneuraldifferentiation,survivalandregeneration.Ithasbeenshowntobecrucialinmodulationofangiogenesis,tissuerepair,embryonicdevelopmentandneuronalfunctioninvivo.
PRODUCTDESCRIPTION
r-bFGFisahumanrecombinantbFGFproteinproducedinRice,whichisasinglenon-glycosylatedpolypeptidechaincontaining146aminoacidsandhasamolecularweightof17kD.Ther-bFGFproteinispurifiedbychromatographictechniques.
Application
Humanembryonicstemcellandvariousotherstemcelllinesuchasmesenchymalstem(stroma)cell(MSC)culture
SOURCE
RiceGrain(OryzaSativa)
Size
50µg
FORMULATION
Lyophilizedpowdercontainingsodiumphosphateandsodiumchloride,pH6.5-7.5
STORAGETEMPERATURE
-20°C
ENDOTOXIN
≤1EU/µg
PURITY
>95%bySDS-PAGE
ACTIVITY
TheED50calculatedbythedose-dependent proliferationofBalb/c3T3cellsexpressingFGFreceptorsis≤1ng/ml,correspondingtoaspecificactivityof(≥1x106)Units/mg.