Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
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Product Name | ZAP-70 (Phospho-Tyr493) Antibody |
Synonyms | 70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70; ZAP70; kinase ZAP70 |
Immunogen | The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr493. |
Immunogen Range | 460-509 |
Clonality | Polyclonal |
Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000 |
Specificity | ZAP-70 (Phospho-Tyr493) Antibody detects endogenous levels of ZAP-70 only when phosphorylated at Tyr493. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage/Stability | Stable at -20°C for at least 1 year. |
NCBI Gene Symbol | ZAP70 |
Database Links |
Gene ID: 7535 Swiss-Prot #: P43403 OMIM #: 176947 Unigene #: Hs.234569 |
Target Modification | Phospho |
Modification Sites | H:Y493 M:Y492 R:Y488 |
Application Images | |
Image 1 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-70 (Phospho-Tyr493) Antibody. The picture on the right is blocked with the phospho peptide. |
Image 2 | Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr493) Antibody. The lane on the right is blocked with the phospho peptide. |