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T4DNALigaseisaligationenzymethatcanbeusedtojoinDNAfragmentsbycatalyzingtheformationofphosphodiesterbondsbetweenjuxtaposed5"phosphateand3"hydroxylterminiindouble-strandedDNAusingATPasacoenzyme.BothbluntandcohesiveendDNAligation,aswellassingle-strandednickrepairofDNA,RNAandDNA/RNA,arepossIBLeusingtheT4DNAligase.