T4DNALigaseisaligationenzymethatcanbeusedtojoinDNAfragmentsbycatalyzingtheformationofphosphodiesterbondsbetweenjuxtaposed5"phosphateand3"hydroxylterminiindouble-strandedDNAusingATPasacoenzyme.BothbluntandcohesiveendDNAligation,aswellassingle-strandednickrepairofDNA,RNAandDNA/RNA,arepossIBLeusingtheT4DNAligase.