ProductDescription
AdvancedBiomatrixoffersPhotoCol®,apurifiedmethacrylatedTypeIbovinecollagenkit.PhotoCol®providesnative-like3Dcollagengelswiththeuniqueattributestobepreparedatvariousconcentrationsandcrosslinkedtoprovidevariousgelstiffness.ThePhotoCol®kitconsistsofpurifiedmethacrylatedTypeIbovinecollagenasthecorecomponentwithothersupportreagentsinthekit.Table1providesalistofthekitcomponents.
| Item | CatalogNumber | PackageSize | StorageTemperature |
| MethacrylatedCollagen | #5198 | 100mg | 2-10°C |
| 20mMAceticAcid | #5079 | 50mL | 2-30°C |
| NeutralizationSolution | #5205 | 10mL | RoomTemperature |
| RutheniumPhotoinitiator | #5246 | 100mg | RoomTemperature |
| SodiumPersulfatePhotoinitiator | #5247 | 500mg | RoomTemperature |
ThemethacrylatedTypeIcollagenisproducedfromtelo-peptideintactbovinecollagenwherethecollagenhasbeenmodifiedbyreactingthefreeamines,primarilytheε-aminesgroupsofthelysineresiduesaswellasthea-aminesgroupsontheN-termini.>20%ofthetotallysineresiduesofthecollagenmoleculehavebeenmethacrylated.Thecollagenisextractedfrombovinehideandcontainsahighmonomercontent.
Thecollagenstartingmaterialwasisolatedfromaclosedherdandpurifiedusingcontrolledmanufacturingprocesses.Abottleof20mMaceticacidsolutionisprovidedtosolubilizethelyophilizedmethacrylatedcollagenatconcentrationsrangingfrom3to8mg/ml.Theneutralizationsolutionconsistsofanalkaline10Xphosphatebufferedsaline(PBS)solutionwhichprovidesphysiologicalsaltsandpHinthefinalmixture.
ThephotoinitiatorconsistsofRutheniumandSodiumPersulfatetobeformulatedin1xPBSorcellculturemediawhichallowsvisIBLelightphotocrosslinkingofthecollagenat400-450nm.Werecommendusingantibioticswiththephotoinitiator,orfilteringthrougha0.2micronbuttonfilterforcellculturework.
| Parameter,Testing,andMethod | MethacrylatedCollagen#5198 |
| SterilizationMethod | Filtration |
| ExtractionMethod | Acid-Telocollagen |
| Form | LyophilizedPowder |
| PackageSize | 100mg |
| DegreeofMethacrylation | >20% |
| StorageTemperature | 2-10°C |
| ShelfLife | Minimumof6monthsfromdateofreceipt |
| ShelfLifeAfterReconstitution | 2Months |
CollagenPurity-SilverStaining | >99% |
| KineticGelTest(Minutes) | <40 |
GelFormationTubeTest(Minutes) | <40 |
ElectrophoreticPattern-CoomassieBlue | Characteristic |
| Sterility-USPmodified | Nogrowth |
| Endotoxin(LAL) | <10.0EU/mL |
| Source | BovineHide |
| HydrogelYoung"sModulusE(Pa) | Characteristic |
DirectionsforUse
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ProductApplications
PhotoCol®MethacrylatedCollagenisaphotocrosslinkabletypeIcollagenfor3Dcellcultureandbioprinting.
Asthemostabundantproteininthebody,collagenisakeycomponentforcellculture.Collagenmethacrylatecanbeusedasarapidlyself-assemblingtypeIcollagentoformcross-linkedhydrogelsfortissueengineering[1].Ithasbeenusedwithmesenchymalstemcells[2],fibroblasts,ADIposederivedstemcells,epithelialcells,andmanymore.
Collagenmethacrylateisusefulforformingscaffoldswithvaryingdegreeofstiffness,byalteringcollagenconcentrationorthedoseofUV-lightexposure[2].Itisalsobeingextensivelycharacterizedforitsusefulnessin3Dbioprinting(extrusion,inkjet,andphotolithographic[3]).
PhotoCol®hasbeenusedforcreated3Dmicroniches[4],aswellasfor3Dbioprintinghumancorneas[5].
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172302/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243547/
http://www.worldscientific.com/doi/abs/10.1142/S2339547817500091
https://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.201700237
https://www.ncbi.nlm.nih.gov/pubmed/29772228
ProductReferences
ReferencesforPhotoCol®:
Isaacson,A.,Swioklo,S.&Connon,C.J.3Dbioprintingofacornealstromaequivalent.ExperimentalEyeResearch173,188–193(2018).
KathrynE.Drzewieckietal,AThermoreversible,photocrosslinkablecollagenbio-inkforfree-formfabricationofscaffoldsforregenerativemedicine,TECHNOLOGY(2017).
Drzewiecki,K.E.etal.MethacrylationInducesRapid,Temperature-Dependent,ReversibleSelf-AssemblyofType-ICollagen.Langmuir30,11204–11211(2014).
Gaudet,I.D.&Shreiber,D.I.CharacterizationofMethacrylatedType-ICollagenasaDynamic,PhotoactiveHydrogel.Biointerphases7,25(2012).
Izadifar,Mohammad,etal."UV-assisted3Dbioprintingofnanoreinforcedhybridcardiacpatchformyocardialtissueengineering."TissueEngineeringPartC:Methods24.2(2018):74-88.
Stoecklin,Celine,etal."ANewApproachtoDesignArtificial3DMicronicheswithCombinedChemical,Topographical,andRheologicalCues."AdvancedBiosystems(2018):1700237.
Mazzocchi,Andrea,etal."OptimizationofcollagentypeI-hyaluronanhybridbioinkfor3Dbioprintedlivermicroenvironments."Biofabrication(2018).
Nguyen,T.U.,Watkins,K.E.&Kishore,V.Photochemicallycrosslinkedcell‐ladenmethacrylatedcollagenhydrogelswithhighcellviABIlityandfunctionality.JournalofBiomedicalMaterialsResearchPartA107,1541–1550(2019).
Mazzocchi,A.etal.PleuralEffusionAspirateforUsein3DLungCancerModelingandChemotherapyScreening.ACSBiomaterialsScience&Engineering5,1937–1943(2019).
Chhetri,A.etal.CellCultureandCocultureforOncologicalResearchinAppropriateMicroenvironments.CurrentProtocolsinChemicalBIOLOGy11,(2019).
ProductCertificateofAnalysis
ProductVideos
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SafetyandDocumentation
SafetyDataSheet
CertificateofOrigin
DeclarationofMaterialSource
ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
