描述Lipofectamine 2000转染试剂是一种多用途转染试剂，可在各种贴壁和悬浮细胞系中提供高效转染。研究人员可使用Lipofectamine 2000试剂进行基于siRNA和shRNA的基因敲除实验及基因表达研究。Lipofectamine 2000是Life Technologies提供的用于siRNA和质粒DNA共转染的zui佳试剂。采用Lipofectamine 2000试剂，您可以实现： 在各种细胞系中具有卓越的转染效率和高重组蛋白表达水平 （表1） 出众的siRNA和质粒DNA共转染性能 在血清存在时亦具有可靠的转染效率 转染后无需更换培养基 适用于高通量应用的可靠性能 建立稳定细胞系的zui佳选择适用于基因表达和基因沉默的高性能转染试剂Lipofectamine 2000试剂适合所有常见细胞系及许多难以转染的细胞系，可用于含有或不含血清的培养基。在基因沉默应用中，Lipofectamine 2000试剂的高效转染提供了很高的基因敲除水平，足以获得令人信服的结果。Lipofectamine 2000试剂可实现siRNA和质粒DNA的高效转染，它是共转染的*试剂。Lipofectamine 2000试剂使用方便 只需将其与核酸混合，加入细胞培养物即可。适合高通量操作Lipofectamine 2000试剂使用简单、快速且具有很高的转染效率，适用于瞬时蛋白表达或高通量RNAi转染。在上述应用中，可轻松建立自动化或机器人系统转染条件。如需了解有关Lipofectamine 2000的实验方案、文献引证及其它产品信息，可登录细胞系数据库。仅供研究使用。不得用于人或动物的治疗或诊断。详细说明常用规格 Cell Type:
Stem Cells,
Primary Cells,
Hard-to-Transfect Cells,
Established Cell Lines
Sample Type （Specific）:RNAi （Synthetic siRNA）,
RNAi Plasmids （shRNA, miR）,
DNA （Plasmid）
Serum Compatible:Yes
Transfection Technique:Lipid-Based Transfection
Product Size:0.75 ml
Shipping Condition:Qualified for Ambient or Wet Ice
Regulatory Statement:For Research Use Only. Not for use in diagnostic procedures.
High Throughput Compatibility:High Throughput-Compatible
按细胞系排列的引文 细胞系名称 有机体类型 组织 HeLa cells Human Cervix CV-1 Primate - Non Human Kidney HT1080 Human Fibroblast C6 cells Rat Brain DU145 Human Prostate LNCaP Human Prostate EcR-293 Human Kidney D3 embryonic stem cells Mouse Embryo NRK Rat Fibroblast BAEC Cow Aorta BE（2）-C Human Neuroblastoma 293 Human Kidney CHO-S Hamster Ovary Neuro 2a, a murine neuroblastoma cell line Mouse Neuroblastoma ECV Human Endothelial COS-7 Primate - Non Human Kidney PC-12 Rat Brain COS-1 Primate - Non Human Kidney HUVEC, HUAEC Human Umbilicus BHK-21 Hamster Kidney SKOV3 Human Ovary Saos-2 Human Bone 293-F Human Kidney CHO K1 Hamster Ovary HT-29 Human Colon Caco-2 cells Human Colon 293S Human Kidney A549 Human Lung VERO Primate - Non Human Kidney K562 Human Bone NIH 3T3-L1, NIH 3T3 Mouse Embryo A-431 Human Skin RKO, Rectal carcinoma cell line Human Colon MDCK Dog Kidney PC-3 Human Prostate Rat Glomerular Mesangial MC Cells Rat Kidney NIH 3T3-L1, NIH 3T3 （） Mouse Embryo HepG2 Human Liver Flp-In 293 Human Kidney JB6 P+ C141 Mouse Skin IMR-90 Human Lung Fibroblasts （normal） Human Fibroblast Keratinocytes Human Keratinocyte Neurons （embryonic cortical） Rat Brain Hepatocytes Rat Liver RAW 264.7 cells, Murine Macrophage Cells Mouse Macrophage Rat-1, Rat Fibroblasts Rat Fibroblast RPE.40 Hamster Kidney Bovine Chromaffin cells Cow Adrenal Gland MRC-5 Human Lung N2A Mouse Neuroblastoma MC3T3-E1 Mouse Osteoblast SK-N-SH, Neuronal Cells Human Brain HAECs Human Aorta T-Rex-293 Human Kidney Neurons （post-natal/adult） Rat Brain TIG Human Fibroblast IMR-32 Human Neuroblastoma HSY-EB Human Other 82-6 Human Fibroblast Flp-In CV-1 Primate - Non Human Kidney LAP3 Mouse Embryo GOTO Human Neuroblastoma SK-N-BE（2） Human Neuroblastoma Daoy Human Other BCEC Human Brain A875 Human Melanoma HMVEC-L Human Lung T-Rex-CHO Hamster Ovary FGC-4 Rat Liver H1299 Human Lung HCT116 Human Colon 293 EBNA Human Kidney 293T Human Kidney MIN6 Mouse Pancreas 回到页首主营：生化试剂、抗体、重组蛋白、细胞生物、分子生物、蛋白研究、微生物、Elisa试剂盒、耗材、标准品、 标准溶液 abcam、ABI、Axygen、corning、信号转导抗体、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R D、Roche、santa、sigma、Thermo等进口品牌 以下为本公司常备现货产品*部分 invitrogen 畅销原装产品
品牌 货号 名称 规格 invitrogen 13778-075 LipofectamineRNAiMAX 0.75ml invitrogen 13778-150 LipofectamineRNAiMAX 1.5ml invitrogen 11668-027 Lipofectamine2000Reagent 0.75ml invitrogen 11668-019 Lipofectamine2000Reagent 1.5ml invitrogen 15596-026 TRlzol Reagent 100ml invitrogen 15596-018 TRlzol Reagent 200ml invitrogen 10296-010 Trizol LSReagent 100ml invitrogen 10296-028 Trizol LSReagent 200ml invitrogen 17504-044 B-27（黄颜色） 10ml invitrogen 12587-010 B-27（黄颜色）不含维生素A 10ml invitrogen 17502-048 N-2（粉红颜色） 5ml invitrogen 18064-014 SuperScript IIReverseTranscriptase 10,000U invitrogen 18080-044 SuperScript IIIReverseTranscriptase 10,000U第二部分 HyClone胎牛血清
品牌 货号 名称 规格 HyClone SH30370.03 AustralianStandardFetalBovineSerum 500ml HyClone SH30406.02 FOETALBOVINESERUM 500ml HyClone SH30401.01 NewZealandCalfSerum 500ml HyClone SH30066.02 FetalClone II 100ml HyClone SH30088.03 STANDARDFETALBOVINESERUM 500ml HyClone SH30118.02 NewbornBovineCalfSerum,U.S.Origin 100ml HyClone SH30070.02 FETALBOVINESERUM 100ml HyClone SH30087.02 COSMICCALF SERUM 100ml HyClone SH30070.03E FETALBOVINESERUMCHARACTERIZSED 500ml 100ml HyClone SH30070.03 FETALBOVINESERUM 500ml HyClone SH30080.03 FetalClone I 500ml HyClone SH30084.03 FOETALBOVINESERUM:CERTIFIEDAUSTRALIAN 500ml HyClone SH30396.03 FETALBOVINESERUMCHARACTERIZED 500ml HyClone SV30087.02 南美源胎牛血清 500ml HyClone sv30087.01 南美源胎牛血清 100ml HyClone SH30071.03 FETALBOVINESERUM 500ml HyClone SH30071.02 FETALBOVINESERUM 100ml HyClone SH30080.02 FetalClone I 100ml HyClone SH30070.02E FETALBOVINESERUMCHARACTERIZSED 100ml HyClone SH30079.02 FETALBOVINESERUM 100ml HyClone SH30068.02 CHARCOAL/DEXTRANTREATEDFBS， 活性炭灭活血清 100ml HyClone SH30068.03 CHARCOAL/DEXTRANTREATEDFBS， 活性炭灭活血清 500ml HyClone SH30066.03 FetalClone II 500ml HyClone SH30109.03 HyCloneFetalClone III 500ml HyClone SH30088.03 STANDARDFETALBOVINESERUM 500ml HyClone SH30088.02 STANDARDFETALBOVINESERUM 100ml HyClone SH30079.03 FETALBOVINESERUM 500ml第二部分 Gibco胎牛血清
品牌 货号 名称 规格 Gibco 12662-029 MSCQUALIFIEDFBS500ML 500ML Gibco 10438-026 FBS,HI500ML 500ML Gibco 10439-024 FBS,ESCELLQUAL.USDAAPPD.500ML 500ML Gibco 10099-141 FBS,AUSTRALIANORIGIN500ML 500ML Gibco 26400-044 FBS,DIALYZED,USAORIGIN500ML 500ML Gibco 26140-079 FBSQUALIFIED,USAORIGIN500ML 500ML Gibco 16141-079 FBS,ESCELLQUAL,USAORIGIN500ML 500ML Gibco 16140-071 FBS,HI500ML 500ML Gibco 16000-044 FBS,CERT,USAORIGIN500ML 500ML Gibco C2027050 FBS,URUGUAYANORIGIN 500ML Gibco 12657-029 巴西胎牛 500ml 如果你对Lipofectamine 2000 Reagent/invitrogen（货号11668-027）说明书感兴趣，想了解更详细的产品信息，填写下表直接与厂家联系：>>> 更多资讯详情请访问蚂蚁淘商城
