Invitrogen R25001 Zeocin 博莱霉素

Bleomycin、Bleocin，BLM

CAS 1056-06-7

争光霉素、博来霉素、硫酸博来霉素、硫酸博莱霉素

货 号

品 名

产地

规格

单位

价格

R25001

zeocin 博莱霉素

Invitrogen

125mg/1.25mL

支

450

R25001

zeocin 博莱霉素

Invitrogen

8*1.25mL /1g

盒

2250

R25005

zeocin 博莱霉素

Invitrogen

50mL /5g

盒

9500

Zeocin? 试剂是博来霉素抗生素家族的一个成员。 其抗性由编码一种 13,665 道尔顿蛋白质的 Sh ble 基因赋予。 在表达这种蛋白质的细胞中，Zeocin? 无法结合和切割细胞 DNA。 Zeocin? 对大多数好氧细胞有效，可用于选择哺乳动物和昆虫细胞系、酵母以及细菌。 选择细胞时，Zeocin? 的用量为 50-2000 ug/ml（通常 300 ug/ml），具体取决于细胞类型。

化学式:C55H83N19O21S2Cu 式量：1137.41 g/mol

Zeocin可选择Shble基因表达的细胞。与现在使用的其他动物细胞标记物无交叉抗性。因此这种抗生素可用来分离对其他筛选剂(如：庆大霉素，潮霉素)有抗性的克隆。Zeocin是一种属于争光霉素家族的糖蛋白抗生素，在体内能作用于大多数细菌(包括E。coli)、真菌(如：酵母菌)、植物细胞、动物细胞、细胞培养级。

使用:●液体Zeocin可用于细胞培养。●用于筛选转化株的Zeocin浓度根据pH值和盐浓度改变。●pH越高，盐浓度越低Zeocin活性越大。●普通大肠杆菌菌株筛选可在25＆micro；g／ml zeocin浓度的低盐LB琼脂培养基中进行，pH调至7.5。●动物细胞筛选：根据不同细胞系从100到750＆micro；g／ml。●植物细胞筛选：根据不同的细胞种类和培养基从50到250＆micro；g／ml。●酵母筛选：取决于种属和培养基，S。cerevisiae Sh ble转化株在50＆micro；g／ml浓度的YPD培养基中

Zeocin? Selection ReagentCat. No. R250-01DescriptionZeocin? Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin? is prevented from binding and cleaving the cellular

DNA. Zeocin? is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin? required for selection will vary between 50-2000 ug/ml （typically 300 ug/ml）depending on the cell type.C55H83N19O21S2CuFormula Weight: 1137.41 g/moleZeocin? is a trademark of CAYLA.The ble resistance gene is the subject of oneormore of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreign equivalents owned by Cayla and licensed to Invitrogen. This product is sold for research purposes only.

For commercial license information, please contact: Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.General SpecificationsConcentration: 100 mg?mLBrand: Zeocin?Form: LiquidProduct Size: 125mgValidated Application: Eukaryotic Selection?Stable Cell Line GenerationBacterial SelectionContents StorageZeocin? is provided

Invitrogen R25001 Zeocin博莱霉素

Bleomycin、Bleocin，BLM

争光霉素、博来霉素、硫酸博来霉素、硫酸博莱霉素

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