other brands: NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscienceproduct type: antibodyproduct name: CaMKII alpha Monoclonal Antibody (6G9)catalog: MA1-048quantity: 100µLprice: US 390clonality: monoclonalhost: mouseconjugate: nonconjugatedclone name: reactivity: human, mouse, ratapplication: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen sectionmore info or order: Invitrogen product webpagePublished Application/Species/Sample/DilutionReferencewestern blot; mouse; 1:5000; loading ...; fig2bMarks C, Shonesy B, Wang X, Stephenson J, Niswender C, Colbran R. Activated CaMKIIα Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization. Mol Pharmacol. 2018;94:1352-1362 pubmedpublisher western blot; human; 1:5000; loading ...; fig2dStephenson J, Wang X, Perfitt T, Parrish W, Shonesy B, Marks C, et al. A Novel Human CAMK2A Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. J Neurosci. 2017;37:2216-2233 pubmedpublisher immunohistochemistry; rat; 1:500; loading ...; fig3cBiró L, Toth M, Sipos E, Bruzsik B, Tulogdi A, Bendahan S, et al. Structural and functional alterations in the prefrontal cortex after post-weaning social isolation: relationship with species-typical and deviant aggression. Brain Struct Funct. 2017;222:1861-1875 pubmedpublisher western blot; rat; loading ...; fig7gBramini M, Sacchetti S, Armirotti A, Rocchi A, Vazquez E, León Castellanos V, et al. Graphene Oxide Nanosheets Disrupt Lipid Composition, Ca(2+) Homeostasis, and Synaptic Transmission in Primary Cortical Neurons. ACS Nano. 2016;10:7154-71 pubmedpublisher western blot; mouse; 1:2000; figs3Bosch C, Muhaisen A, Pujadas L, Soriano E, MARTINEZ A. Reelin Exerts Structural, Biochemical and Transcriptional Regulation Over Presynaptic and Postsynaptic Elements in the Adult Hippocampus. Front Cell Neurosci. 2016;10:138 pubmedpublisher immunohistochemistry; rat; 1:200; fig5western blot; rat; 1:2000; fig5Györffy B, Gulyassy P, Gellén B, Völgyi K, Madarasi D, Kis V, et al. Widespread alterations in the synaptic proteome of the adolescent cerebral cortex following prenatal immune activation in rats. Brain Behav Immun. 2016;56:289-309 pubmedpublisher immunohistochemistry; human; 1:100; fig3Mantoan Ritter L, Macdonald D, Ritter G, Escors D, Chiara F, Cariboni A, et al. Lentiviral expression of GAD67 and CCK promoter-driven opsins to target interneurons in vitro and in vivo. J Gene Med. 2016;18:27-37 pubmedpublisher immunocytochemistry; rat; 5.8 ug/ml; fig6Cisternas P, Louveau A, Bueno S, Kalergis A, Boudin H, Riedel C. Gestational Hypothyroxinemia Affects Glutamatergic Synaptic Protein Distribution and Neuronal Plasticity Through Neuron-Astrocyte Interplay. Mol Neurobiol. 2016;53:7158-7169 pubmed immunohistochemistry - frozen section; mouse; 1:100; fig8Giordano C, Vinet J, Curia G, Biagini G. Repeated 6-Hz Corneal Stimulation Progressively Increases FosB/ΔFosB Levels in the Lateral Amygdala and Induces Seizure Generalization to the Hippocampus. PLoS ONE. 2015;10:e0141221 pubmedpublisher western blot; mouse; 1:2000; fig8Tajerian M, Leu D, Yang P, Huang T, Kingery W, Clark J. Differential Efficacy of Ketamine in the Acute versus Chronic Stages of Complex Regional Pain Syndrome in Mice. Anesthesiology. 2015;123:1435-47 pubmedpublisher western blot; mouse; 1:2000; fig1Tom Dieck S, Kochen L, Hanus C, Heumüller M, Bartnik I, Nassim Assir B, et al. Direct visualization of newly synthesized target proteins in situ. Nat Methods. 2015;12:411-4 pubmedpublisher western blot; mouse; 1:4000-1:10,000; fig2cBaucum A, Shonesy B, Rose K, Colbran R. Quantitative proteomics analysis of CaMKII phosphorylation and the CaMKII interactome in the mouse forebrain. ACS Chem Neurosci. 2015;6:615-31 pubmedpublisher immunocytochemistry; rat; 1:500; fig4Isensee J, Wenzel C, Buschow R, Weissmann R, Kuss A, Hucho T. Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit. PLoS ONE. 2014;9:e115731 pubmedpublisher immunocytochemistry; mouse; 1:200immunohistochemistry; mouse; 1:200western blot; mouseCooper M, Koleske A. Ablation of ErbB4 from excitatory neurons leads to reduced dendritic spine density in mouse prefrontal cortex. J Comp Neurol. 2014;522:3351-62 pubmedpublisher immunocytochemistry; mouseDai X, Iwasaki H, Watanabe M, Okabe S. Dlx1 transcription factor regulates dendritic growth and postsynaptic differentiation through inhibition of neuropilin-2 and PAK3 expression. Eur J Neurosci. 2014;39:531-47 pubmedpublisher western blot; human; 1:3000; fig2Shonesy B, Wang X, Rose K, Ramikie T, Cavener V, Rentz T, et al. CaMKII regulates diacylglycerol lipase-? and striatal endocannabinoid signaling. Nat Neurosci. 2013;16:456-63 pubmedpublisher western blot; humanSanz Clemente A, Gray J, Ogilvie K, Nicoll R, Roche K. Activated CaMKII couples GluN2B and casein kinase 2 to control synaptic NMDA receptors. Cell Rep. 2013;3:607-14 pubmedpublisher immunohistochemistry - frozen section; rat; 1:1000immunohistochemistry; rat; 1:1000Calu D, Kawa A, Marchant N, Navarre B, Henderson M, Chen B, et al. Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats. J Neurosci. 2013;33:214-26 pubmedpublisher immunohistochemistry; rat; 1:100Fanous S, Goldart E, Theberge F, Bossert J, Shaham Y, Hope B. Role of orbitofrontal cortex neuronal ensembles in the expression of incubation of heroin craving. J Neurosci. 2012;32:11600-9 pubmedpublisher western blot; rat; 1:1000Hodas J, Nehring A, Höche N, Sweredoski M, Pielot R, Hess S, et al. Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT). Proteomics. 2012;12:2464-76 pubmedpublisher western blot; human; 1:5000Sakrikar D, Mazei Robison M, MERGY M, Richtand N, Han Q, Hamilton P, et al. Attention deficit/hyperactivity disorder-derived coding variation in the dopamine transporter disrupts microdomain targeting and trafficking regulation. J Neurosci. 2012;32:5385-97 pubmedpublisher western blot; mouseWills T, Klug J, Silberman Y, Baucum A, Weitlauf C, Colbran R, et al. GluN2B subunit deletion reveals key role in acute and chronic ethanol sensitivity of glutamate synapses in bed nucleus of the stria terminalis. Proc Natl Acad Sci U S A. 2012;109:E278-87 pubmedpublisher western blot; mousePronsato L, Boland R, Milanesi L. Testosterone exerts antiapoptotic effects against H2O2 in C2C12 skeletal muscle cells through the apoptotic intrinsic pathway. J Endocrinol. 2012;212:371-81 pubmedpublisher immunocytochemistry; rat; 1:400western blot; rat; 1:400Otmakhov N, Lisman J. Measuring CaMKII concentration in dendritic spines. J Neurosci Methods. 2012;203:106-14 pubmedpublisher western blot; humanJiao Y, Jalan Sakrikar N, Robison A, Baucum A, Bass M, Colbran R. Characterization of a central Ca2+/calmodulin-dependent protein kinase IIalpha/beta binding domain in densin that selectively modulates glutamate receptor subunit phosphorylation. J Biol Chem. 2011;286:24806-18 pubmedpublisher immunohistochemistry - frozen section; mouse; 1:200immunohistochemistry; mouse; 1:200western blot; mouse; 1:2000Mouton Liger F, Thomas S, Rattenbach R, Magnol L, Larigaldie V, Ledru A, et al. PCP4 (PEP19) overexpression induces premature neuronal differentiation associated with Ca(2+) /calmodulin-dependent kinase II-? activation in mouse models of Down syndrome. J Comp Neurol. 2011;519:2779-802 pubmedpublisher western blot; human; 1:2,000Bowton E, Saunders C, Erreger K, Sakrikar D, Matthies H, Sen N, et al. Dysregulation of dopamine transporters via dopamine D2 autoreceptors triggers anomalous dopamine efflux associated with attention-deficit hyperactivity disorder. J Neurosci. 2010;30:6048-57 pubmedpublisher western blot; mouse; 1:4,000Brigman J, Wright T, Talani G, Prasad Mulcare S, Jinde S, Seabold G, et al. Loss of GluN2B-containing NMDA receptors in CA1 hippocampus and cortex impairs long-term depression, reduces dendritic spine density, and disrupts learning. J Neurosci. 2010;30:4590-600 pubmedpublisher western blot; mouseBaucum A, Jalan Sakrikar N, Jiao Y, Gustin R, Carmody L, Tabb D, et al. Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach. Mol Cell Proteomics. 2010;9:1243-59 pubmedpublisher western blot; mouseKojima N, Hanamura K, Yamazaki H, Ikeda T, Itohara S, Shirao T. Genetic disruption of the alternative splicing of drebrin gene impairs context-dependent fear learning in adulthood. Neuroscience. 2010;165:138-50 pubmedpublisher western blot; rat; 1:2,000Soule J, Penke Z, Kanhema T, Alme M, Laroche S, Bramham C. Object-place recognition learning triggers rapid induction of plasticity-related immediate early genes and synaptic proteins in the rat dentate gyrus. Neural Plast. 2008;2008:269097 pubmedpublisher western blot; mouseLee T, Karl R, Moosmang S, Lenhardt P, Klugbauer N, Hofmann F, et al. Calmodulin kinase II is involved in voltage-dependent facilitation of the L-type Cav1.2 calcium channel: Identification of the phosphorylation sites. J Biol Chem. 2006;281:25560-7 pubmed immunohistochemistry; rat; 1:400Nyffeler M, Zhang W, Feldon J, Knuesel I. Differential expression of PSD proteins in age-related spatial learning impairments. Neurobiol Aging. 2007;28:143-55 pubmed immunocytochemistry; humanHerrmann T, Morita C, Lee K, Kusner D. Calmodulin kinase II regulates the maturation and antigen presentation of human dendritic cells. J Leukoc Biol. 2005;78:1397-407 pubmed immunocytochemistry; rat; 1:800-1:2000Harms K, Craig A. Synapse composition and organization following chronic activity blockade in cultured hippocampal neurons. J Comp Neurol. 2005;490:72-84 pubmed immunohistochemistry; rat; 1:700Kalita K, Szymczak S, Kaczmarek L. Non-nuclear estrogen receptor beta and alpha in the hippocampus of male and female rats. Hippocampus. 2005;15:404-12 pubmedNihonmatsu I, Ohkawa N, Saitoh Y, Okubo Suzuki R, Inokuchi K. Selective targeting of mRNA and the following protein synthesis of CaMKIIα at the long-term potentiation-induced site. Biol Open. 2020;9: pubmedpublisherMatos M, Bosson A, Riebe I, Reynell C, Vallée J, Laplante I, et al. Astrocytes detect and upregulate transmission at inhibitory synapses of somatostatin interneurons onto pyramidal cells. Nat Commun. 2018;9:4254 pubmedpublisherGiannotti G, Barry S, Siemsen B, Peters J, McGinty J. Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine-seeking. J Neurosci. 2018;38:8956-8966 pubmedpublisherYamazaki H, Sasagawa Y, Yamamoto H, Bito H, Shirao T. CaMKIIβ is localized in dendritic spines as both drebrin-dependent and drebrin-independent pools. J Neurochem. 2018;146:145-159 pubmedpublisherLarsson M. Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn. Brain Struct Funct. 2018;223:1437-1457 pubmedpublisherWang S, Stanika R, Wang X, Hagen J, Kennedy M, Obermair G, et al. Densin-180 Controls the Trafficking and Signaling of L-Type Voltage-Gated Cav1.2 Ca2+ Channels at Excitatory Synapses. J Neurosci. 2017;37:4679-4691 pubmedpublisherKuiper L, Frohmader K, Coolen L. Maladaptive Sexual Behavior Following Concurrent Methamphetamine and Sexual Experience in Male Rats is Associated with Altered Neural Activity in Frontal Cortex. Neuropsychopharmacology. 2017;42:2011-2020 pubmedpublisherKool M, van de Bree J, Bodde H, Elgersma Y, van Woerden G. The molecular, temporal and region-specific requirements of the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in mouse locomotion. Sci Rep. 2016;6:26989 pubmedpublisherLeão R, Cruz F, Vendruscolo L, de Guglielmo G, Logrip M, Planeta C, et al. Chronic nicotine activates stress/reward-related brain regions and facilitates the transition to compulsive alcohol drinking. J Neurosci. 2015;35:6241-53 pubmedpublisherMessaoudi E, Kanhema T, Soule J, Tiron A, Dagyte G, da Silva B, et al. Sustained Arc/Arg3.1 synthesis controls long-term potentiation consolidation through regulation of local actin polymerization in the dentate gyrus in vivo. J Neurosci. 2007;27:10445-55 pubmedGong R, Park C, Abbassi N, Tang S. Roles of glutamate receptors and the mammalian target of rapamycin (mTOR) signaling pathway in activity-dependent dendritic protein synthesis in hippocampal neurons. J Biol Chem. 2006;281:18802-15 pubmedSchlief M, Craig A, Gitlin J. NMDA receptor activation mediates copper homeostasis in hippocampal neurons. J Neurosci. 2005;25:239-46 pubmedSilasi G, Diaz Heijtz R, Besplug J, Rodriguez Juarez R, Titov V, Kolb B, et al. Selective brain responses to acute and chronic low-dose X-ray irradiation in males and females. Biochem Biophys Res Commun. 2004;325:1223-35 pubmedMiller C, Marshall J. Altered prelimbic cortex output during cue-elicited drug seeking. J Neurosci. 2004;24:6889-97 pubmedAtkins C, Nozaki N, Shigeri Y, Soderling T. Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II. J Neurosci. 2004;24:5193-201 pubmedDong Y, Rosenberg H. Prolonged changes in Ca2+/calmodulin-dependent protein kinase II after a brief pentylenetetrazol seizure; potential role in kindling. Epilepsy Res. 2004;58:107-17 pubmedDong Y, Rosenberg H. Brief seizure activity alters Ca2+/calmodulin dependent protein kinase II dephosphorylation and subcellular distribution in rat brain for several hours. Neurosci Lett. 2004;357:95-8 pubmedGerzanich V, Ivanova S, Van Der Heijden M, Zhou H, Simard J. Trans-cellular proliferating cell nuclear antigen gene activation in cerebral vascular smooth muscle by endothelial oxidative injury in vivo. Arterioscler Thromb Vasc Biol. 2003;23:2048-54 pubmedYoshii A, Sheng M, Constantine Paton M. Eye opening induces a rapid dendritic localization of PSD-95 in central visual neurons. Proc Natl Acad Sci U S A. 2003;100:1334-9 pubmedMenegon A, Verderio C, Leoni C, Benfenati F, Czernik A, Greengard P, et al. Spatial and temporal regulation of Ca2+/calmodulin-dependent protein kinase II activity in developing neurons. J Neurosci. 2002;22:7016-26 pubmedFong D, Rao A, Crump F, Craig A. Rapid synaptic remodeling by protein kinase C: reciprocal translocation of NMDA receptors and calcium/calmodulin-dependent kinase II. J Neurosci. 2002;22:2153-64 pubmedPeng X, Jia Z, Zhang Y, Ware J, Trimble W. The septin CDCrel-1 is dispensable for normal development and neurotransmitter release. Mol Cell Biol. 2002;22:378-87 pubmedZhang T, Johnson E, Gu Y, Morissette M, Sah V, Gigena M, et al. The cardiac-specific nuclear delta(B) isoform of Ca2+/calmodulin-dependent protein kinase II induces hypertrophy and dilated cardiomyopathy associated with increased protein phosphatase 2A activity. J Biol Chem. 2002;277:1261-7 pubmedAllison D, Chervin A, Gelfand V, Craig A. Postsynaptic scaffolds of excitatory and inhibitory synapses in hippocampal neurons: maintenance of core components independent of actin filaments and microtubules. J Neurosci. 2000;20:4545-54 pubmedOuyang Y, Rosenstein A, Kreiman G, Schuman E, Kennedy M. Tetanic stimulation leads to increased accumulation of Ca(2+)/calmodulin-dependent protein kinase II via dendritic protein synthesis in hippocampal neurons. J Neurosci. 1999;19:7823-33 pubmedZhang W, Vazquez L, Apperson M, Kennedy M. Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus. J Neurosci. 1999;19:96-108 pubmedOchiishi T, Yamauchi T, Terashima T. Regional differences between the immunohistochemical distribution of Ca2+/calmodulin-dependent protein kinase II alpha and beta isoforms in the brainstem of the rat. Brain Res. 1998;790:129-40 pubmedBarria A, Muller D, Derkach V, Griffith L, Soderling T. Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation. Science. 1997;276:2042-5 pubmedKuiper G, Carlsson B, Grandien K, Enmark E, Haggblad J, Nilsson S, et al. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology. 1997;138:863-70 pubmedPatton B, Molloy S, Kennedy M. Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies. Mol Biol Cell. 1993;4:159-72 pubmedCho K, Hunt C, Kennedy M. The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein. Neuron. 1992;9:929-42 pubmedChang P, Sculco F, Yates V. An in vivo and in vitro study of infectious laryngotracheitis virus in chicken leukocytes. Avian Dis. 1977;21:492-500 pubmed Applications: Immunofluorescence: 1:100, Immunohistochemistry: Assay dependent, Immunoprecipitation: Assay dependent, Western Blot: 0.5-1 µg/mLSpecies: Mouse, RatClone: Isotype: Storage: -20° C, Avoid Freeze/Thaw CyclesDescription: CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.Immunogen: Partially purified, rat CaM kinase II.Format: LiquidApplications w/Dilutions: Immunofluorescence: 1:100, Immunohistochemistry: Assay dependent, Immunoprecipitation: Assay dependent, Western Blot: 0.5-1 µg/mLAliases: Calcium/calmodulin-dependent protein kinase type 2, CaMKIImore info or order: Invitrogen product webpageThermo Fisher Scientific81 Wyman StreetWaltham, MA USA 02451https://www.thermofisher.com800-678-5599headquarters: USA

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