描述
qubit®(早期称为Quant-iT™)dsDNABR分析试剂盒(Qubit®dsDNABRAssayKit)专门设计用于在Qubit®2.0荧光仪(Q32866),但也可以在任何荧光计或荧光酶标仪中使用。该试剂盒对双链DNA(dsDNA)有高度的选择性，不会定量RNA，可以定量浓度为100pg/µl到1000ng/µl的样品。试剂盒提供了浓缩的分析试剂，稀释缓冲液和预制的DNA标准。您只需用提供的缓冲液稀释试剂，添加样品(体积在1µL到20µL范围内),然后使用Qubit®2.0荧光仪读取浓度。该试剂盒对于常见污染物，如盐，游离的核苷酸，溶剂，去污剂，蛋白质的耐受性极好。
注意:所有的Qubit®分析试剂盒同样适用于Qubit®1.0荧光仪。
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Description
TheQubitdsDNABRAssayKitisdesignedspecificallyforusewiththeQubitFluorometer.Theassayishighlyselectivefordouble-strandedDNA(dsDNA)overRNAandisdesignedtobeaccurateforinitialsampleconcentrationfrom100pg/µl–1,000ng/µl.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedDNAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1µland20µlisacceptable),andreadtheconcentrationusingtheQubitFluorometer.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,orproteinarewelltoleratedintheassay.
WhichproducttochoosefordsDNABR(BroadRange)quantitation?
•For1–20samples:usethisQubitdsDNABRAssayKitwiththeQubitFluorometer
•For20–2000samples:usetheQuant-iTdsDNABRAssayKitwithmicroplatereader
Notes:
1.AllQubitassaykitscanbeusedwiththeQubit1.0,Qubit2.0,Qubit3,andQubit4fluorometers.
2. 500µLthin-walledPCRtubes arerequiredbutnotincluded.ForResearchUseOnly.Notforuseindiagnosticprocedures.
Specifications
| SampleType(General): | dsDNA |
|---|---|
| ForUseWith(Equipment): | Qubit®Fluorometer |
| QuantitationRange: | 2-1000ng |
| NumberofReactions: | 100reactions |
| ProductLine: | Quant-iT™, Qubit™ |
| ProductSize: | 100assays |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storeinrefrigerator(2–8°C)andprotectfromlight.
