产品时间：2017-12-07 简要描述：B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书；由上海慧颖生物提供，库存现货，价格优惠，另有N2添加剂促销热卖中，B-27 添加剂是无血清添加剂，用于海马神经元和其他中枢神经系统（CNS）神经元的生长和保持其短期或长期活性。咨询！ B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书描述B-27® 添加剂是无血清添加剂，用于海马神经元和其他中枢神经系统（CNS）神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体，可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用，用于神经细胞培养而不需要再添加饲养层细胞。B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用，我们提供其他配方的B-27® 添加剂.质量控制检测B-27® 添加剂 进行了18天妊娠的原代大鼠（Sprague Dawley） 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。仅供研究使用。除非另有说明，不得用于任何动物或人类治疗或诊断。详细说明B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书常用规格Cell Type:Neuronal CellsProduct Size:10 mlClassification:Serum-FreeOther Additives:Vitamin A,Antioxidant Cocktail,InsulinPurity or Quality Grade:Cell Culture GradeGreen Features:Sustainable packagingShipping Condition:Dry IceRegulatory Statement:For Research Use Only. Not for use in diagnostic procedures.引文和参考文献 （17）D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.作者Congar P; Bergevin A; Trudeau LE杂志J Neurophysiol （2002） 87:1046-1102产品用途Using a culture system in which isolated DAergic neurons establish self-innervating synapses （\"autapses\"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）.ID:PN64425Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.作者Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;杂志Neuron （2002） 33:789-804产品用途Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37 C in a 5% CO2 humidified incubator.ID:PN47418Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.作者Kabos Peter; Kabosova Andrea; Neuman Toomas;杂志J Biol Chem （2002） 277:8763-8769产品用途Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen） ID:PN63471Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.作者Lesuisse Christian; Martin Lee J;杂志J Neurobiol （2002） 51:9-23产品用途A long-term cell culture system was used to study maturation, aging, and death of cortical neurons.ID:PN60461An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.作者Joseph-Bravo Patricia; P rez-Mart nez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;杂志Brain Res Brain Res Protoc （2002） 9:93-104产品用途Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal propertiesID:PN62028更多..回到页首按细胞系排列的引文细胞系名称有机体类型组织SH-Sy5YHumanBrainFetal neuronsRatBrainNeurons （post-natal/adult）RatBrainNeurons （superior cervical ganglia - SCG）RatBrainNeurons （cortical）MouseOtherNeural Stem CellsRatBrain慧颖生物主营：生化试剂、抗体、重组蛋白、细胞生物、分子生物、蛋白研究、微生物、Elisa试剂盒、耗材、标准品、 标准溶液 abcam、ABI、Axygen、corning、CST、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R D、Roche、santa、sigma、Thermo等进口品牌 以下为相关本公司常备现货产品*部分 invitrogen 原装产品品牌货号名称规格invitrogen13778-075LipofectamineRNAiMAX0.75mlinvitrogen13778-150LipofectamineRNAiMAX1.5mlinvitrogen11668-027Lipofectamine2000Reagent0.75mlinvitrogen11668-019Lipofectamine2000Reagent1.5mlinvitrogen15596-026TRlzol®Reagent100mlinvitrogen15596-018TRlzol®Reagent200mlinvitrogen10296-010Trizol®LSReagent100mlinvitrogen10296-028Trizol®LSReagent200mlinvitrogen17504-044B-27（黄颜色）10mlinvitrogen12587-010B-27（黄颜色）不含维生素A10mlinvitrogen17502-048N-2（粉红颜色）5mlinvitrogen18064-014SuperScript®IIReverseTranscriptase10,000Uinvitrogen18080-044SuperScript®IIIReverseTranscriptase10,000U第二部分 HyClone胎牛血清品牌货号名称规格HyCloneSH30370.03AustralianStandardFetalBovineSerum500mlHyCloneSH30406.02FOETALBOVINESERUM500mlHyCloneSH30401.01NewZealandCalfSerum500mlHyCloneSH30066.02FetalClone®II100mlHyCloneSH30088.03STANDARDFETALBOVINESERUM500mlHyCloneSH30118.02NewbornBovineCalfSerum,U.S.Origin100mlHyCloneSH30070.02FETALBOVINESERUM100mlHyCloneSH30087.02COSMICCALF®SERUM100mlHyCloneSH30070.03EFETALBOVINESERUMCHARACTERIZSED500ml100mlHyCloneSH30070.03FETALBOVINESERUM500mlHyCloneSH30080.03FetalClone®I500mlHyCloneSH30084.03FOETALBOVINESERUM:CERTIFIEDAUSTRALIAN500mlHyCloneSH30396.03FETALBOVINESERUMCHARACTERIZED500mlHyCloneSV30087.02南美源胎牛血清500mlHyClonesv30087.01南美源胎牛血清100mlHyCloneSH30071.03FETALBOVINESERUM500mlHyCloneSH30071.02FETALBOVINESERUM100mlHyCloneSH30080.02FetalClone®I100mlHyCloneSH30070.02EFETALBOVINESERUMCHARACTERIZSED100mlHyCloneSH30079.02FETALBOVINESERUM100mlHyCloneSH30068.02CHARCOAL/DEXTRANTREATEDFBS，活性炭灭活血清100mlHyCloneSH30068.03CHARCOAL/DEXTRANTREATEDFBS，活性炭灭活血清500mlHyCloneSH30066.03FetalClone®II500mlHyCloneSH30109.03HyCloneFetalClone®III500mlHyCloneSH30088.03STANDARDFETALBOVINESERUM500mlHyCloneSH30088.02STANDARDFETALBOVINESERUM100mlHyCloneSH30079.03FETALBOVINESERUM500ml第二部分 Gibco胎牛血清品牌货号名称规格Gibco12662-029MSCQUALIFIEDFBS500ML500MLGibco10438-026FBS,HI500ML500MLGibco10439-024FBS,ESCELLQUAL.USDAAPPD.500ML500MLGibco10099-141FBS,AUSTRALIANORIGIN500ML500MLGibco26400-044FBS,DIALYZED,USAORIGIN500ML500MLGibco26140-079FBSQUALIFIED,USAORIGIN500ML500MLGibco16141-079FBS,ESCELLQUAL,USAORIGIN500ML500MLGibco16140-071FBS,HI500ML500MLGibco16000-044FBS,CERT,USAORIGIN500ML500MLGibcoC2027050FBS,URUGUAYANORIGIN500MLGibco12657-029巴西胎牛500ml

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