Description:

Ratinterferon-gammaELISA:
Assayrange:4-256pg/ml
Sensitivity:4pg/ml
Specificity:nat.andrec.ratIFN-gamma
Calibration:rec.ratIFN-gamma
Typeofsample:serum,plasma,culturesupernatant

Contents:

• Coatingantibody
• Biotinylateddetectorantibody
• Cytokinestandard(5x)
• Streptavidin-HRP(SPP)conjugate
• TMBsubstratesolution
• Stopsolution
• CytokinestABIlizationbuffer(CSB)
• BSAstocksolution(10%)
• Tween-20
• ELISAplateswithcoverslips

Referenceslist:

• Thebeta2-adrenergicagoNISTsalbutamolpotentiatesoralinductionoftolerance,suppressingadjuvantarthritisandantigen-specificimmunity.Cobelens,PieterM,etal.J.Immunol.2002Nov1;169:5028-35
• Invitroadrenergicmodulationofcellularimmunefunctionsinexperimentalautoimmuneencephalomyelitis.Haerter,Katharina,etal.J.Neuroimmunol.2004Jan;146:126-32
• Neuralsubstratesforbehaviorallyconditionedimmunosuppressionintherat.Pacheco-López,Gustavo,etal.J.Neurosci.2005Mar2;25:2330-7
• IndividualdifferencesinbehaviorofinbredLewisratsareassociatedwithseverityofjointdestructioninadjuvant-inducedarthritis.Sajti,Enikö,etal.BrainBehav.Immun.2004Nov;18:505-14
• Maternaldeprivationofratpupsincreasesclinicalsymptomsofexperimentalautoimmuneencephalomyelitisatadultage.Teunis,MarcAT,etal.J.Neuroimmunol.2002Dec;133:30-8
• Mechanicalventilationofhealthyratssuppressesperipheralimmunefunction.Vreugdenhil,HA,etal.Eur.Respir.J.2004Jan;23:122-8

U-CyTech为干扰素提供基于CPE的抗病毒测定（AVA）测试，也称为细胞病变效应（CPE）抑制测定。在该体外生物测定中，将一个单位定义为将病毒感染的CPE降低50％的干扰素的量。将测试样品的抗病毒生物活性与国际或实验室标准制剂进行比较，并以国际单位（IU）或实验室单位（LU）/ ml表示。 人，大鼠和小鼠干扰素的生物活性分别通过人肺上皮细胞（HEp-2），大鼠胚胎细胞（RATEC）和小鼠成纤维细胞（L929）受到水泡性口腔炎病毒（VSV）攻击来测量。