Description:

Monocyte chemotactic protein-1 (MCP-1), also called CCL2, is an inflammatory chemokine that plays important roles in recruiting monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. MCP-1 also involves in obesity and insulin resistance by the induction of an inflammatory response (macrophage infiltration) in fatty tissue. In addition, MPC-1 has been found in the joints of people with rheumatoid arthritis where may serve to recruit macrophages and perpetuate the inflammation in the joints.

Principle:

The ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a anti-rat cytokine antibody for immobilization on the microtiter wells and anti-rat cytokine antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules of the cytokine being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of the cytokine is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

Data:

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