Description:

Obesity increases the risk for the metabolic syndrome, diabetes, hypertension, atherosclerosis, and thrombosis. A number of proteins have been identified to be relevant to the development of the metabolic syndrome, diabetes, and cardiovascular disease with obesity. Plasma concentrations of these proteins are usually measured by ELISA. To systematically examine the effects, Signosis developed an ELISA strip which allows simultaneous determination of 8 obersity cytokines.The difference of these proteins between two samples can be determined through data comparison. Therefore, it facilitates the discovery of the change of these proteins in different samples.

Principle:

Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measures simultaneously. The test sample reacts simultaneously with two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is then added and causes a blue color change. The reaction is then terminated with Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.

Data:

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