Description:

Cytokines are extracellular signaling proteins produced by different cell types that act on target cells to modulate diverse cellular functions, such as recruiting specific cell types to the site of inflammation, increasing the activation and survival of immune cells, or suppressing cellular activity. Inflammation is the response of tissue to injury. During both acute and chronic inflammatory processes, a variety of soluble factors are involved in the cellular infiltrate, the cellular activation, and the systemic responses to inflammation. Cytokines are major determinants of inflammatory responses. Most cytokines are multifunctional molecules that elicit their effects locally or systemically in an autocrine or paracrine manner. Cytokines are involved in extensive networks that involve synergistic as well as antagonistic interactions and exhibit both negative and positive regulatory effects on various target cells. Therefore, profiling the expression pattern of cytokines provides a valuable insight to the underlying immunological mechanisms. Signosis’ Mouse Inflammation ELISA Strip allows quantitatively profiling and measuring 8 inflammation cytokines.

Principle:

Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measures simultaneously. The test sample reacts simultaneously with two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is then added and causes a blue color change. The reaction is then terminated with Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.

Data:

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