Description:
TF targets its binding site at upstream of TATA promoter to induce the expression of luciferase gene. It results in induction of luciferase enzyme activity. Through comparison of luciferase activity in two samples, up- or down-reguated TF can be quantitatively measured.Principle:
TF targets its binding site at upstream of TATA promoter to induce the expression of luciferase gene. It results in induction of luciferase enzyme activity. Through comparison of luciferase activity in two samples, up- or down-reguated TF can be quantitatively measured.
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