Description:

Epidermalgrowthfactorreceptor(EGFR)isacell-surfacereceptorwithintrinsicintracellularprotein-tyrosinekinase(TK)activity.LigandbindinginducesEGFRdimerizationandphosphorylation,leADIngtotheactivationofEGFRsignalingpathway.Inseveralmalignanciessuchasnon-smallcelllungcancer(NSCLC),EGFRsignalingisderegulatedduetomutationsinEGFR,whichresultsinuncontrolledproliferationandmigrationoftumorcells.EGFRmutationscanleadto“oncogene-addicted”cancers,wherethetumorcellsdependonthemutatedEGFRforcellsurvivalandmalignantphenotype. OneofthemostcommonEGFRmutationsfoundinhumanpatientsisL858Rsubstitutioninexon21,withintheactivationloopofEGFR.PatientswiththismutationaresensitivetoEGFRtyrosinekinaseinhibitors(TKIs)suchasgefitiniborerlotinib,whereaspatientswithwildtypeEGFRarenotsensitivetoTKI.AnotherclinicallyrelevantmutationassociatedwithacquiredgefitinibanderlotinibresistanceisT790M,foundinexon20.CellsexpressingEGFRwithbothL858RandT790Mmutationsareresistanttoinducedapoptosisinthepresenceofgefitiniborerlotinib. 

Data:

Dose-dependentgrowthinhibitionofBaF3cellsharboringEGFRDel19(EL-011),EGFRIns19(EL-009)andrepresentativeErlotinibresistantcellline,EGFRDel19-T790M(EL-014).Thecellsweretreatedwiththeindicateddoseoferlotinibfor24hoursandcellsviABIlitywasmeasuredusingSignosisCVCreagent.EGFRDel19stablyexpressingBaF3cells(EL-011)remains14-foldmoresensitivetoTKIerlotinib(IC50=0.34)thanitsresistancederivativeEGFRDel19-T790MstablyexpressingBaF3cells(EL-014)(IC50=4.9).

 

Signosis基因表达  是利用来自基因的信息指导功能性基因产物合成的过程。这些产品通常是蛋白质。基因表达分析对于理解基因及其蛋白产物的功能，以及对影响基本生物学过程的复杂调控网络的清晰了解至关重要。Signosis  提供了许多用于基因表达研究的测定产品，包括用于监控由TF上调或下调的基因表达的cDNA板阵列，以及用于分析基因表达的单细胞PCR试剂盒，可直接分析细胞无需RNA分离即可进行裂解。 SARS-CoV-2实时PCR检测试剂盒单细胞基因表达分析印迹分析RNA的cDNA板阵列细胞裂解液的cDNA板阵列