AGAP2 Antibody Properties Description Anti-AGAP2 (RABBIT) Antibody - 200-401-946 Host Rabbit Target Species Human Known Cross Reactivity human, mouse Clonality Polyclonal Application ELISA : 1:40,000 - 1:200,000 Western Blot : 1:5,000 - 1:20,000 Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format IgG Label Unconjugated Concentration 2.0 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 100 µL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName AGAP2 Relevant Links NCBI - 7661962UniProtKB - Q99490GeneID - 116986

AGAP2 Antibody Description

Background This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). AGAP2 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. Anti-AGAP2 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research. Synonyms rabbit anti-AGAP2 antibody, AGAP-2, AGAP 2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody, centaurin gamma1 antibody, Centg1 antibody Immunogen This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein. Immunogen Type Recombinant Protein Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Application Note This protein A purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to AGAP2 protein by western blotting in the appropriate cell lysate or extract. Purity/Specifity This protein A purified antibody is directed against human AGAP2 protein. The product was purified from monospecific antiserum by protein A chromatography.  A BLAST analysis was used to suggest cross reactivity with AGAP2 protein from mouse and rat based on 93% and 95% protein:protein homologies, respectively, with the immunizing sequence.  Reactivity against homologues from other sources is not known. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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