5-mC Antibody Properties Description Anti-5-mC (MOUSE) Monoclonal Antibody - 200-301-U98 Host Mouse Target Species Human Known Cross Reactivity Human, Mouse, Other (wide range) Clonality Monoclonal IgG1 Application IF Microscopy : 1:500 ImmunoPrecipitation: 1-2 µg/IP Other Dilution: FISH dilution: 1:200 - 1:1,000. Dot Blot: 1:600 Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG1 Label Unconjugated Concentration 2.4 mg/ml by UV absorbance at 280 nm Buffer 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 Preservative 0.05% (w/v) Sodium Azide Stabilizer None

5-mC Antibody Description

Background 5-methyl Cytidine is a modified nucleotide found in the DNA of both plants and vertebrates. Anti-5-mC Antibody is ideal for research in Epigenetics, specifically DNA methylation, due to its applicability to methylation analysis. Synonyms 5-methylcytidine, 5-methyl Cytidine Immunogen Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methyl Cytidine. Immunogen Type Other Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-5-mC Antibody is suitable for Dot Blotting, Fluorescence In Situ Hybridization (FISH), Immunofluorescence and Methylated DNA Immunoprecipitation (MeDIP). Specific conditions for reactivity should be optimized by the end user. Purity/Specifity Anti-5-mC Antibody was purified by Protein A chromatography. Cross-reactivity with 5-mC from other sources has not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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