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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
AKT3WesternChemiluminescentBlottingKitProperties Description AKT3WesternBlotKit-KCAE75 Host Mouse TargetSpecies Human KnownCrossReactivity human Clonality Monoclonal9A5.H9.G7IgG1kappa Application WesternBlot:UserOptimized OtherDilution:UserOptimized ProductType Kit KitType ChemiluminescentWesternBlotKit PhysicalState n/a ShippingCondition DryIce Format IgG1 Label Unconjugated Preservative WashbuffersMUSTNOTcontainSODIUMAZIDEorotherinhibitorsofperoxidaseactivity! StABIlizer None GeneName AKT3 RelevantLinks GeneID-10000 UniProtKB-Q9Y243 NCBI-Q9Y243 AKT3ChemiluminescentWBlotKitProtocol |
AKT3WesternChemiluminescentBlottingKitDescription
Background
AKT3WesternBlotingkitallowsforthedetectionofendogenousproteinlevelsofhumanAKT3presentincelllysatesprovidedbytheuser.Asapositivecontrol,thiskitincludesaMDA-MB468wholecelllysateproventocontainAKT3.AfterproteinseparationbySDS-PAGEandtransfer,themembraneisprobedwithRockland"soptimizedAnti-AKT3antibody.Detectionofthemembraneboundantibody-antigencomplexisachievedbytheadditionofasecondaryantibodyconjugatedtotheenzymehorserADIshperoxidase.Theenzymereactswithaspecializedformulationofluminol,anextremelysensitive,non-radioactivesubstratethatemitslightandallowsvisualizationusingX-rayfilmorotherimagingmethods,includinghighlysensitiveCCDcamerasandimagingsystems.AKT3WesternBlotingisidealforinvestigatorsinvolvedinCellSignaling,Cancer,NeuroscienceandSignalTransductionresearch.
Synonyms
AKT3westernblotting,AKT-3,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt3antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma,westernblotingkit,ChemiluminescentWB,WesternBlottingKit,ChemiluminescentKit,PeroxidaseKit,ImmunoblottingKit
StorageCondition
Seekitinsertforcompleteinstructions.
ApplicationNote
UseRocklandImmunochemicals’Anti-AKT3ChemiluminescentKitforWesternBlottingfordetectionofhumanAKT3nativeandrecombinantproteinsbywesternblot.Expectabandapproximately56kDainsizecorrespondingtoAKT3protein.Thiskitisusefulforbothwesternblottinganddotblottingmethods.Pleasereadtheentireproductinsertpriortouse.
Purity/Specifity
RocklandImmunochemicals’ChemiluminescentWesternBlotKitforAKT3combinesallofthenecessaryreagentswitharapidprovenprotocolandtheextremelyhighsignaldetectionofourluminolchemiluminescentsubstrateforthedetectionofnativeand/orrecombinanthumanAKT3protein.Thekitisdesignedtodetectbothunphosphorylatedandphosphorylatedformsoftheprotein.CrossreactivitywithAKT3fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows100%identitytohuman,mouse,andrat,therefore,crossreactivityisexpected.Cross-reactivitywithAKT1andAKT2hasnotbeendetermined.Thiskitcontainssufficientsubstrateforupto5miniblotsat7.5x8cm2(1,800cm2)andisstableforatleast1yearwhenstoredasindicated.
DisclaimerNote-General
ThismaterialissubjecttoproprietaryrightsandissoldunderlicensefromROCKLANDIMMUNOCHEMICALSInc.Thisproductislicensedforsaleonlyfor"research-use"only.Thereisnoimpliedlicensehereunderforanycommercialuse.FemtoMax®isatrademarkofROCKLANDIMMUNOCHEMICALSInc.Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
