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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
AKT2WesternChemiluminescentBlottingKitProperties Description AKT2WesternBlotKit-KCAE71 Host Rat TargetSpecies Human KnownCrossReactivity human Clonality Monoclonal16G11.E8IgG2akappa Application WesternBlot:UserOptimized OtherDilution:UserOptimized ProductType Kit KitType ChemiluminescentWesternBlotKit PhysicalState n/a ShippingCondition DryIce Format IgG2a Label Unconjugated Preservative WashbuffersMUSTNOTcontainSODIUMAZIDEorotherinhibitorsofperoxidaseactivity! StABIlizer None GeneName AKT2 RelevantLinks UniProtKB-P31751 AKT2ChemiluminescentWBKitProtocol |
AKT2WesternChemiluminescentBlottingKitDescription
Background
AKT2WesternBlotingkitallowsforthedetectionofendogenousproteinlevelsofhumanAKT2presentincelllysatesprovidedbytheuser.Asapositivecontrol,thiskitincludesaMDA-MB468wholecelllysateproventocontainAKT2.AfterproteinseparationbySDS-PAGEandtransfer,themembraneisprobedwithRockland"soptimizedAnti-AKT2antibody.Detectionofthemembraneboundantibody-antigencomplexisachievedbytheadditionofasecondaryantibodyconjugatedtotheenzymehorserADIshperoxidase.Theenzymereactswithaspecializedformulationofluminol,anextremelysensitive,non-radioactivesubstratethatemitslightandallowsvisualizationusingX-rayfilmorotherimagingmethods,includinghighlysensitiveCCDcamerasandimagingsystems.AKT2WesternBlotingisidealforinvestigatorsinvolvedinCellSignaling,Cancer,NeuroscienceandSignalTransductionresearch.
Synonyms
AKT2westernblotting,AKT-2,AKT2antibody,AKT-2,PKBantibody,PKBbetaantibody,PKBBETAantibody,PRKBBantibody,ProteinkinaseAkt2antibody,ProteinkinaseBbetaantibody,RAC-betaserine/threonine-proteinkinase,RAC-PK-beta,westernblotingkit,ChemiluminescentWB,WesternBlottingKit,ChemiluminescentKit,PeroxidaseKit,ImmunoblottingKit
StorageCondition
Seekitinsertforcompleteinstructions.
ApplicationNote
UseRocklandImmunochemicals’Anti-AKT2ChemiluminescentKitforWesternBlottingfordetectionofhumanAKT2nativeandrecombinantproteinsbywesternblot.Expectabandapproximately56kDainsizecorrespondingtoAKT2protein.Thiskitisusefulforbothwesternblottinganddotblottingmethods.Pleasereadtheentireproductinsertpriortouse.
Purity/Specifity
RocklandImmunochemicals’ChemiluminescentWesternBlotKitforAKT2combinesallofthenecessaryreagentswitharapidprovenprotocolandtheextremelyhighsignaldetectionofourluminolchemiluminescentsubstrateforthedetectionofnativeand/orrecombinanthumanAKT2protein.Thekitisdesignedtodetectbothunphosphorylatedandphosphorylatedformsoftheprotein.CrossreactivitywithAKT2fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows100%identitytohuman,mouse,andrat,therefore,crossreactivityisexpected.Cross-reactivitywithAKT1andAKT3hasnotbeendetermined.Thiskitcontainssufficientsubstrateforupto5miniblotsat7.5x8cm2(1,800cm2)andisstableforatleast1yearwhenstoredasindicated.
DisclaimerNote-General
ThismaterialissubjecttoproprietaryrightsandissoldunderlicensefromROCKLANDIMMUNOCHEMICALSInc.Thisproductislicensedforsaleonlyfor"research-use"only.Thereisnoimpliedlicensehereunderforanycommercialuse.FemtoMax®isatrademarkofROCKLANDIMMUNOCHEMICALSInc.Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
