返回页首用于食品和饮料的组胺 EIA 试剂盒$610.00SKUFS35产品概述

组胺广泛存在于食品中。食物的微生物分解（腐败）会产生大量的组胺。我们的组胺免疫测定是独一无二的，因为它不需要对样品进行预处理以形成组胺衍生物，从而使该方法更简单并且更少证明由于不完全转化为衍生物而引起的变化。

产品文件MSDS FR35 MSDSSpec Sheet FR35 Spec相关产品ProductCAT.#SizePriceHuman Prorenin, C-terminal 8x His tagRN340.1 mg$315.00Human alpha-thrombinCT361.0 mg$335.00HORAC AssayTA30Kit$280.00GST M5-5, Recombinant HumanGS590.1 mg$460 .00人类Prorenin, NativeRN330.1 mg355.00 美元 An injectable, click-crosslinked, cytomodulin-modified hyaluronic acid hydrogel for cartilage tissue engineering AbstractThis is the first report, to our knowledge, of the preparation of an injectable in situ鈥揻orming click-crosslinked hyaluronic acid (Cx-HA) hydrogel (Cx-HA-CM) containing chemical immobilized cytomodulin-2 (CM), a chondrogenic differentiation factor, and on the utility of human periodontal ligament stem cells (hPLSCs) as a cell source for cartilage tissue engineering. hPLSCs served here as a stem cell source tolerant to ex vivo manipulation. CM induced in vitro chondrogenic differentiation of hPLSCs comparable to induction with traditional TGF-尾. Cx-HA was prepared via a click-reaction between tetrazine-modified HA and transcyclooctene-modified HA. Cx-HA displayed significantly more features of a stiff hydrogel than HA. Cx-HA had a three-dimensional porous interconnected structure, absorbed a large volume of biological medium, and showed excellent biocompatibility. In contrast to HA, the Cx-HA hydrogel persisted in vitro and in vivo for an extended period, as evidenced by in vivo near-infrared fluorescence imaging. CM covalently linked to Cx-HA (Cx-HA-CM) remained inside Cx-HA for a prolonged period compared with CM physically loaded onto Cx-HA [Cx-HA (+CM)]. Cx-HA-CM also caused better chondrogenic differentiation of hPLSCs, as evidenced by Alcian blue and Safranin O staining, and greater increases in the expression of type II collagen, glycosaminoglycan content and SOX9, aggrecan, and type 2伪1 collagen mRNA levels. Thus, compared to Cx-HA (+CM), the hPLSC-loaded Cx-HA-CM hydrogel induced greater chondrogenic differentiation of hPLSCs via CM that was retained in the hydrogel for a much longer period of time. IntroductionDamaged articular cartilage has limited spontaneous healing potential, and failure to repair such cartilage often leads to osteoarthritis, pain, and malfunction of the affected joint1,2,3. Although many approved therapeutic procedures for restoring the structure of damaged articular cartilage are available, including microfracture, cell implantation, and tissue grafts, these procedures often do not produce robust and well-repaired articular cartilage4,5,6.Cartilage tissue engineering is considered a promising modality for effective repair of damaged cartilage tissue7,8. The procedure most often used in cartilage tissue engineering involves a suitable combination of seeded cells, a biocompatible scaffold, and biological factors that support cartilage formation. The success of cartilage tissue regeneration depends on the individual and/or combined characteristics of the cells, the biological factors, and the scaffold9. In this work, we attempted to design a suitable combination of cells and biological factors and a suitable scaffold for cartilage tissue engineering.First, we chose human periodontal ligament stem cells (hPLSCs) as a cell source for the cartilage tissue engineering in this work because hPLSCs can easily be harvested in large quantities from teeth obtained during dental repair or surgical procedures. Additionally, hPLSCs have been proposed as a self-renewing and multipotent cell source; they have a high proliferation rate during ex vivo expansion and can be cryopreserved for long periods. Furthermore, hPLSCs are capable of differentiation into a chondrogenic lineage and also possess other differentiation potential; this property makes hPLSCs attractive for studies of cartilage tissue engineering10,11,12.Next, we chose a hyaluronic acid (HA)-based hydrogel for cartilage tissue engineering in this work because HA is a naturally occurring biopolymer that is present in almost all mammalian tissues; due to its biocompatibility, biodegradability, and nontoxicity, it has been widely utilized in a variety of medical treatments13. In cartilage tissue regeneration, HA has been employed in various scaffold formats such as cream, film, and foam14,15,16,17,18. Some research groups have reported that HA scaffolds stimulate in vitro chondrogenesis of mesenchymal stromal cells (MSCs) and that constructs that are in vitro tissue-engineered via chondrogenic differentiation of MSCs in HA scaffolds have properties similar to those of native cartilage19,20.Injectable in situ鈥揻orming hydrogels have been widely used as scaffolds for cartilage tissue engineering21,22,23,24,25. Injectable in situ鈥揻orming hydrogel systems easily and quantitatively incorporate various cells and biological factors after simple mixing at room temperature and can be injected as a liquid to easily form a hydrogel in situ.Several natural materials, including HA, cellulose, and collagen, have been proposed as injectable in situ鈥揻orming hydrogels for cartilage tissue engineering25. An injectable HA hydrogel can temporarily maintain its elastic and viscose integrity as a scaffold under physiological conditions. HA hydrogels show good capacity for water absorption and high swelling ability through water absorption. Consequently, the use of an injectable HA hydrogel as a scaffold can create a suitable three-dimensional environment for faster growth of loaded cells by allowing the diffusion of various biological nutrients. Furthermore, the injectable HA hydrogel can itself enhance cartilage-specific synthesis of extracellular matrix by stem cells, as reported elsewhere26,27. Thus, injectable in situ鈥揻orming HA hydrogels appear promising for use in cartilage tissue engineering. However, once injected, HA hydrogels gradually and completely disappear under physiological conditions due to their short residence time.It was recently shown that the biorthogonal Diels鈥揂lder click reaction between tetrazine (Tet) and transcyclooctene (TCO) can proceed rapidly under physiological conditions even in the absence of an external catalyst28,29,30,31,32. To our knowledge, there are very few published studies on the use of injectable click-cross-linked HA hydrogels in cartilage tissue engineering of hPLSCs. In this work, we designed Tet-modified HA (HA-Tet) and TCO-modified HA (HA-TCO) and used them in situ to form a click-crosslinked HA (Cx-HA) hydrogel with prolonged residence time.Transforming growth factor beta (TGF-尾) is an important regulator of crucial cellular processes involved in early embryonic development, cell growth, differentiation, motility, and apoptosis33. In addition, TGF-尾 controls the chondrogenic differentiation of MSCs. Thus, TGF-尾 has been added as a key component to nearly all chondrogenic media; however, it requires careful storage to prevent denaturation and is quite costly due to expensive manufacturing steps.If bioactive short peptides that mimic the action of full-length TGF-尾 can be designed, they would be attractive alternatives to full-length TGF-尾 owing to their greater stability on storage and cost-effectiveness. For these reasons, the development of short bioactive peptides that mimic the effects of TGF-尾 has recently gained importance. Cytomodulin-2 (CM) is one such TGF-尾鈥搈imicking short peptide34,35,36,37. Although the exact mechanism through which CM mimics TGF-尾 is not known, it has been reported that the CM peptide acts via a mechanism similar to that of TGF-尾 in which it interacts with cell surface TGF-尾 receptors through the stable and bend structure of the CM peptide (-Val-Ala-)37. As a consequence, the TGF-尾鈥搈imicking CM peptide plays a role similar to that of TGF-尾 and enhances the expression of collagen I and pro-collagen I in wound healing. Additionally, the CM peptide has the advantages of longer shelf life and lower cost. Furthermore, one research group has reported that, like TGF-尾, the CM peptide can effectively induce the chondrogenic differentiation of human MSCs (hMSCs)35. For these reasons, we chose the CM peptide in this work as a biological factor for cartilage tissue engineering based on hPLSCs.For the purpose of cartilage tissue engineering via hPLSCs, the CM peptide can be physically loaded onto a Cx-HA hydrogel by simple mixing. However, the physically CM-loaded Cx-HA [Cx-HA (+CM)] does not provide an efficacious and stable source of the peptide for the required period of time due to the rapid release of the peptide from the Cx-HA hydrogel. Chemical immobilization of the CM peptide on the Cx-HA hydrogel instead of physical loading is likely to prolong the residence time of CM. Thus, we hypothesized that Cx-HA hydrogel (Cx-HA-CM) that was chemically modified with CM would enhance the chondrogenic differentiation of hPLSCs due to the resulting prolonged retention of CM inside the Cx-HA hydrogel (Fig. 1). To our knowledge, no published studies have described the use of in situ click-crosslinked Cx-HA-CM for the purpose of cartilage tissue engineering using hPLSCs.Fig. 1: Schematic images showing injection of a formulation of hPLSC-loaded HA-Tet-CM and hPLSC-loaded HA-TCO-CM for chondrogenic differentiation of hPLSCs in an in vivo鈥揻ormed Cx-HA-CM hydrogel.(Image was drawn by S.H.P. using Adobe Photoshop 7.0 software)Full size imageThe objectives of this study were to answer the following questions: (1) Can CM induce chondrogenic differentiation of hPLSCs, which are a cell source for cartilage tissue engineering? (2) Can an injectable click-crosslinkable-Cx-HA-CM鈥揵ased hydrogel formulation be introduced into the body to produce a scaffold for hPLSCs in a minimally invasive manner? (3) Can Cx-HA-CM synergistically induce chondrogenic differentiation of hPLSCs more effectively than Cx-HA (+CM)? Such an injectable Cx-HA-CM鈥揵ased hydrogel formulation holds promise for satisfying the unmet need for (pre)clinical repair of damaged articular cartilage using safe and inexpensive bioactive short CM peptides as well as for enhancing the chondrogenic differentiation of hPLSCs as a resource for cartilage tissue engineering.Materials and methodsMaterialsHyaluronic acid (HA) (1 MDa, Humedix, Anyang, Korea), 4,6-dimethoxy-1,3,5-triazin-2yl-4-methylmorpholinium chloride (DMTMM, Sigma, St. Louis, MO, USA), methyltetrazine-PEG4-amine hydrochloride salt (Click Chemistry Tools, AZ, USA), trans-cyclooctene-amine hydrochloride salt (Click Chemistry Tools, AZ, USA), 1,4-diaminobutane (Sigma), cytomodulin-2 (CM, sequence LIANAK) (Peptron, Daejeon, Korea), fluorescein isothiocyanate鈥揷onjugated CM (CM-FI) (Peptron, Daejeon, Korea), bromodeoxyuridine (BrdU, Sigma), BrdU antibody (Dako, Glostrup, Denmark), goat anti-mouse IgG antibody conjugated with Alexa Fluor庐 594 (Invitrogen, CA, USA), collagen type 2伪 antibody (Abcam, Cambridge, UK), dimethylmethylene blue (DMMB), glycine, NaCl, acetic acid, and chondroitin sulfate from shark cartilage (all from Sigma), PicoGreen reagent (Invitrogen, Carlsbad, CA, USA), 位 DNA standard and TRIzol Reagent (Invitrogen, CA, USA), SuperScript庐 III First-Strand Kit (Invitrogen), Power SYBR庐 Green (Applied Biosystems, UK), primers for amplification of SOX9, aggrecan (ACAN), and type 2伪1 collagen (COL2A1) (Genotech, Daejeon, Korea) were used as received.Preparation of hPLSCs for in vitro and in vivo chondrogenic differentiationFresh hPLSCs were prepared according to previously described methods38. All of the procedures used in this study were approved by the Institutional Review Board for Human Subjects Research and Ethics Committee of Inha Hospital (approval No. IUH IRB 12-150).For preparation of PKH-labeled hPLSCs, PKH67 labeling was performed according to the protocol provided by the manufacturer (Sigma, MO, USA). Briefly, hPLSCs were detached using trypsin-EDTA (Gibco, NY, USA), and the suspended hPLSCs were centrifuged for 5鈥塵in at 2000鈥塺pm and 4鈥壜癈. The supernatant was discarded, and the cell pellet was resuspended in serum-free 伪-minimal essential medium (伪-MEM; Gibco, NY, USA) to remove serum proteins. The resuspended cells were centrifuged again, the supernatant was removed, and the cell pellet was resuspended in 1鈥塵L of Diluent C. PKH67 was diluted to a concentration of 4鈥壝椻€?0鈥?鈥塎 in Diluent C. Next, 1鈥塵L of the PKH67 solution was added to the cell suspension, and the mixture was incubated for 5鈥塵in in the dark at room temperature. To terminate the PKH67 labeling reaction, 2鈥塵L of fetal bovine serum (FBS, Gibco, NY, USA) was added to the mixture followed by incubation for 1鈥塵in. The PKH67-labeled hPLSCs were centrifuged for 5鈥塵in at 2000鈥塺pm and 4鈥壜癈. The supernatant was discarded, and the cell pellet was washed twice with 10鈥塵L of complete medium to ensure the removal of unbound dye. The PKH67-labeled hPLSCs were then used in in vitro experiments.For preparation of bromodeoxyuridine (BrdU)-labeled hPLSCs, passage-four hPLSCs were cultured in a 75-cm2 tissue culture flask (BD Falcon, CA, USA) in 伪-MEM (Gibco, NY, USA) containing 15% FBS (Gibco, NY, USA), 2鈥塵mol/L l-glutamine (Gibco, NY, USA), 100鈥壩糾ol/L ascorbic-acid-2-phosphate (Sigma, MO, USA), 1% of a penicillin-streptomycin solution (PS, Gibco, NY, USA), and 100鈥壩糾ol/L BrdU for 24鈥塰. The hPLSCs were then recovered from the flask by washing it out twice with 10鈥塵L PBS. The BrdU-labeled hPLSCs were subjected to in vivo experiments.For in vitro chondrogenic differentiation, passage-five hPLSCs (5鈥壝椻€?05) were seeded in 15-mL conical tubes (BD Falcon, MA, USA) and centrifuged at 2000鈥塺pm and 4鈥壜癈 for 5鈥塵in to form a pellet of hPLSCs. The pelleted hPLSCs were incubated for 24鈥塰 at 37鈥壜癈 in a humidified atmosphere containing 5% CO2. Each cell pellet was treated with one of three culture media: 1鈥塵L of a control medium (Cont) (伪-MEM鈥?鈥?5% FBS鈥?鈥?% l-glutamine鈥?鈥?% penicillin-streptomycin鈥?鈥?.1% ascorbic acid), 1鈥塵L of TGF chondrogenic medium (伪-MEM鈥?鈥?% non-essential amino acids鈥?鈥?% % penicillin-streptomycin鈥?鈥?% ITS鈥?鈥?00鈥塶M dexamethasone 1.25鈥塵g/mL BSA鈥?鈥?.35鈥壩糶/mL linoleic acid鈥?鈥?0 渭g/mL ascorbic acid鈥?鈥?0 渭g/mL l-proline鈥?鈥?0鈥塶g/mL TGF-尾), and 1鈥塵L of a chondrogenic medium (CM) (伪-MEM鈥?鈥?% nonessential amino acids鈥?鈥?% penicillin-streptomycin鈥?鈥?% ITS鈥?鈥?00鈥塶M dexamethasone鈥?鈥?.25鈥塵g/mL BSA鈥?鈥?.35鈥壜礸/mL linoleic acid鈥?鈥?0鈥壜礸/mL ascorbic acid鈥?鈥?0鈥壜礸/mL l-proline鈥?鈥?00鈥壜礛 CM). The pelleted hPLSCs were cultured for 4 weeks with replacement of the culture medium every 3 days. To confirm chondrogenic induction, hPLSCs were fixed with 4% paraformaldehyde after 2 and 4 weeks of cultivation. The fixed hPLSCs were embedded in paraffin, and the paraffin block was cut into 4-渭m-thick slices. The paraffinized slides were deparaffinized twice with xylene at 70鈥壜癈 for 2鈥塰.For hematoxylin and eosin (H E) staining, the deparaffinized slides were hydrated in 100, 95, 70, and 60% ethanol and washed with deionized water (DW). The slides were then washed in running tap water, stained for 5鈥塵in with a hematoxylin solution (Sigma), and washed with DW. The slides were immersed in 0.1% acidic alcohol for 10鈥塻, washed with DW, and incubated in 1% aqueous ammonia for 1鈥塵in; after washing with DW, the slides were stained for 3鈥塵in with an eosin solution (Sigma). The stained slides were fixed and mounted using mounting medium (Muto Pure Chemicals, Tokyo, Japan).For Safranin O (SO) staining, the hematoxylin-stained slides described in the previous paragraph were stained with Fast Green solution for 5鈥塵in and washed with 1% acetic acid for 10鈥塻. The slides were then stained with 0.1% SO for 5鈥塵in and immersed in 95% ethanol, 100% ethanol and xylene for 5鈥塵in each. The stained slides were fixed and mounted using mounting medium.For immunofluorescent staining of type 2 collagen, the slides were hydrated for 5鈥塵in and treated with citrate buffer (Sigma, St. Louis, MO, USA) at 120鈥?30鈥壜癈 for 10鈥塵in. The slides were washed with PBS and PBS-T (0.05% Tween 20 in PBS) and blocked with 5% bovine serum albumin (BSA; Millipore, Billerica, MA, USA) and 5% horse serum (HS, Gibco, Auckland, New Zealand) in PBS for 90鈥塵in at 37鈥壜癈. The sections were incubated for 16鈥塰 at 4鈥壜癈 with an antibody against collagen type 2 alpha 1 (Abcam, Cambridge, UK) diluted in antibody diluent (DaKo, Glostrup, Denmark) (1:500), washed with PBS and PBS-T, and incubated with a secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor庐 594; Invitrogen, USA) (1:200) for 3鈥塰 at room temperature in the dark. The slides were then washed with PBS and PBS-T, counterstained with DAPI in DW (1:1000) for 20鈥塵in, and mounted using fluorescence mounting solution (DaKo, Glostrup, Denmark). The slides were visualized using an LSM 710 microscope (Carl Zeiss Microimaging GmbH, G枚ttingen, Germany) and analyzed using ZEN 2009 software (Carl Zeiss Microimaging GmbH, G枚ttingen, Germany).Gene expression during in vitro chondrogenic differentiation of hPLSCsRNA was isolated from the pellet of hPLSCs using TRIzol Reagent (Invitrogen, CA, USA). RNA was quantified on an ND-1000 spectrophotometer (NanoDrop Technologies, DE, USA) at 260鈥塶m, and the concentration and quality of RNA were confirmed by agarose gel electrophoresis. The extracted RNA samples (50鈥塶g) were reverse-transcribed using the SuperScript III First-Strand Kit (Invitrogen). PCR amplification and real-time fluorescence detection of SOX9 (SOX9), aggrecan (ACAN), and type 2伪1 collagen (COL2A1) were performed on a Chromo4 Real-Time PCR System (Bio-Rad, CA, USA) with Power SYBR Green (Applied Biosystems, UK). Expression of the genes SOX9, ACAN, and COL2A1 was normalized to the expression of 伪-tubulin (AT). All samples were assayed in triplicate. Data analysis was conducted using the comparative threshold cycle (Ct) method (2鈭捨斘擟t) for relative quantification. The PCR primers (GenoTech, Daejeon, Korea) were as follows: SOX9, 5鈥?ACACACAGCTCACTCGACCTTG-3鈥? 5鈥?GGGAATTCTGGTTGGTCCTCT-3鈥? ACAN, 5鈥?GAGATGGAGGGTGAGGTC-3鈥? 5鈥?ACGCTGCCTCGGGCTTC-3鈥? COL2A1, 5鈥?GGACTTTTCTCCCCTCTCT-3鈥? 5鈥? GACCCGAAGGTCTTACAGGA-3鈥?Preparation of tetrazine-modified HA (HA-Tet) and transcyclooctene-modified HA (HA-TCO)HA was dispersed in DW at a final concentration of 10鈥塵g/mL. DMTMM (35鈥塵g, 0.13鈥塵mol) was individually added to the HA solution, and the mixture was stirred for 30鈥塵in (Fig. 1). Transcyclooctene-amine hydrochloride salt (6.6鈥塵g, 0.025鈥塵mol) and methyltetrazine-PEG4-amine hydrochloride salt (9.1鈥塵g, 0.025鈥塵mol) were individually added to the HA solution (10鈥塵g/mL); the mixture was then stirred for 24鈥塰 at room temperature to prepare tetrazine-modified-HA (HA-Tet) and transcyclooctene-modified-HA (HA-TCO). The HA-Tet and HA-TCO samples were dialyzed for 72鈥塰 to remove unreacted Tet and TCO. After dialysis, the obtained HA-Tet and HA-TCO were lyophilized in a freeze dryer (FD 8508, Ilshinlab, Daejeon, Korea). The yields of HA-TCO and HA-Tet were 91% and 90%, respectively. The structures of the obtained compounds were confirmed by 1H-NMR spectroscopy. Elemental analysis of the amine groups in HA-Tet (C: 39.4%, H: 5.9%, N: 4.5%) and HA-TCO (C: 39.5%, H: 5.7%, N: 3.95%) showed almost quantitative introduction of Tet and TCO.Preparation of crosslinked HA (Cx-HA)The obtained HA-Tet and HA-TCO were individually dispersed in PBS at a final concentration of 20鈥塵g/mL. The HA-Tet and HA-TCO solutions were separately drawn into the individual compartments of a dual-barrel syringe. The Cx-HA was prepared by simultaneous injection of the material in each compartment of the dual-barrel syringe into a vial for subsequent characterization.Characterization of HA, HA-Tet, HA-TCO, and Cx-HATo investigate the structure of the obtained material, the 1H nuclear magnetic resonance (NMR) spectra of HA, HA-Tet, HA-TCO, and Cx-HA were recorded using a Mercury Plus 400 instrument (Varian) with D2O in the presence of tetramethylsilane as an internal standard.The rheological properties of HA, HA-Tet, HA-TCO, Cx-HA and Cx-HA that had been subjected to swelling in PBS for 52鈥塰 were examined using a rheometer (MCR 102, Anton Paar, Ostfildern, Germany) with a Peltier temperature-controlled bottom plate and a 25.0-mm stainless steel parallel plate measuring system. All measurements were conducted at a gap length of 0.3鈥塵m at 25鈥壜癈. The time sweep was 0鈥?00鈥塻, the oscillating frequency was 1鈥塇z, and the 纬 amplitude was 2%. The frequency sweep was conducted at an oscillating frequency of 0.1鈥?0鈥塇z and a 纬 amplitude of 2%. The strain sweep was performed at 0.1鈥?000% oscillating strain and 1鈥塇z oscillating frequency. The storage modulus (G鈥?, loss modulus (G鈥?, viscosity (畏), and phase angle (tan 未) were calculated by the instrument鈥檚 software.Determination of the swelling properties of Cx-HATwo hundred microliters of the prepared Cx-HA and HA (4鈥塵g Cx-HA or HA) were individually added to a 20-mL vial; 10鈥塵L of PBS was then added, and the mixture was incubated at 37鈥壜癈. At predetermined time points, the PBS that had not been absorbed by the Cx-HA was removed from the vial. The remaining PBS on the surface of the vial was removed using clean wipes (Yuhan-Kimberly, Seoul, Korea). The vial was then weighed to obtain the weight of wet Cx-HA. Each sample of Cx-HA hydrogel was then lyophilized in a freeze dryer until the residue reached a constant weight, and the vial was weighed to determine the weight of the dry Cx-HA.The swelling ratio was defined as follows:$${mathrm{Swelling}},{mathrm{ratio}},left( {mathrm{% }} right){mathrm{ = [(weight}},{mathrm{of}},{mathrm{wet}},{mathrm{Cx - HA}} - ,{mathrm{weight}},{mathrm{of}},{mathrm{dry}},{mathrm{Cx - HA) div (weight}},{mathrm{of}},{mathrm{dry}},{mathrm{Cx - HA)] times 100}}{mathrm{.}}$$Preparation of near-infrared (NIR) fluorescence鈥搇abeled HAZW800-1C was prepared according to previously reported methods39. ZW800-1C (10.5鈥塵g, 0.01鈥塵mol) was solubilized in 10鈥塵L of DW. DMTMM (3.3鈥塵g, 0.012鈥塵mol) was added to the ZW800-1C solution, and the mixture was stirred at room temperature for 30鈥塵in. 1,4-diaminobutane (15.4鈥塵g, 0.18鈥塵mol) in 1鈥塵L of DW was added dropwise to the activated ZW800-1C solution, and the solution was then stirred for 24鈥塰. The amine-modified ZW800-1C was dialyzed for 3 days to remove unreacted DMTMM and 1,4-diaminobutane and then lyophilized in a freeze dryer. Amine-modified ZW800-1C (2.86鈥塵g, 0.0025鈥塵mol) and DMTMM (830鈥壜礸, 0.003鈥塵mol) were individually added to 5-mL aliquots of 10鈥塵g/mL HA-Tet or HA-TCO solutions prepared in DW, and the mixtures were stirred at room temperature. The obtained NIR-labeled HA-Tet and HA-TCO solutions were dialyzed for 3 days and then lyophilized in a freeze dryer.Preparation of CM-modified crosslinked HA (Cx-HA-CM)DMTMM (0.6鈥塵g, 0.002鈥塵mol) was individually added to 5鈥塵L of HA-Tet (10鈥塵g/mL) and HA-TCO (10鈥塵g/mL) solutions, and the mixtures were stirred for 1鈥塰. The CM solution (1.6鈥塵g) was individually added to the activated HA-Tet and HA-TCO solutions, and the mixtures were then stirred at room temperature for 24鈥塰. The obtained CM-modified HA-Tet and CM-modified HA-TCO were dialyzed for 3 days and then lyophilized using a freeze dryer.For in vitro and in vivo imaging, CM-FI was covalently attached to HA-Tet and HA-TCO prepared according to the above method. The obtained CM-FI鈥搈odified HA-Tet and CM-FI鈥搈odified HA-TCO were dissolved at concentrations of 10鈥塵g/mL in PBS. The fluorescence intensity of the solutions of CM-FI鈥搈odified HA-Tet and CM-FI鈥搈odified HA-TCO was analyzed on an FP-8200 spectrofluorometer (JASCO, MD, USA), and the results were used to determine the amount of CM-FI that was chemically immobilized on the HA-Tet and HA-TCO. The fluorescence intensity was measured at an excitation wavelength of 495鈥塶m and an emission wavelength of 516鈥塶m.In vitro CM-FI release from CM-FI鈥搇oaded Cx-HA [Cx-HA (+CM-FI)] and CM-FI鈥搈odified Cx-HA [Cx-HA-CM-FI]Each solution of HA-Tet (+CM-FI) and HA-TCO (+CM-FI), or CM-FI鈥搈odified HA-Tet and CM-FI鈥搈odified HA-TCO was individually prepared. Aliquots of the solutions (200鈥壜礚) were individually added to 20-mL vials and mixed to prepare CM-FI-loaded Cx-HA [from HA-Tet (+CM-FI)] and HA-TCO (+CM-FI) and CM-FI鈥搈odified Cx-HA [from CM-FI鈥搈odified HA-Tet and CM-FI鈥搈odified HA-TCO]. The Cx-HA (+CM-FI) and Cx-HA-CM-FI hydrogels contained the same amount of CM-FI (0.62鈥塵g/mL, 0.6鈥塵M). Next, 5鈥塵L of fresh PBS was added to the vials containing Cx-HA (+CM-FI), and the Cx-HA-CM-FI hydrogel was shaken at 100鈥塺pm in an incubator at 37鈥壜癈 for 28 days. At the end of this period, 2鈥塵L of PBS was removed from each vial and replaced with the same volume of fresh PBS. The amount of released CM-FI was measured using an FP-8200 spectrofluorometer. The fluorescence intensity was measured at an excitation wavelength of 495鈥塶m and an emission wavelength of 516鈥塶m. Three independent release experiments were performed on the Cx-HA (+CM-FI) and Cx-HA-CM-FI hydrogels. The amount of CM-FI cumulatively released in vitro was calculated by comparison with standard calibration curves based on known concentrations of CM-FI.To determine in vitro CM-FI degradation in PBS, CM-FI (100鈥壜礸) was dissolved in 1鈥塵L of PBS in a vial and shaken at 100鈥塺pm in an incubator at 37鈥壜癈 for up to 28 days. At 7, 14, 21, and 28 days, 200鈥壜礚 of the solution was withdrawn from each vial, and 200鈥壜礚 of fresh PBS was immediately added to restore the volume. The amount of CM-FI in PBS was measured using an FP-8200 spectrofluorometer.In vitro viability of hPLSCs in Cx-HAEach solution of HA-TCO and HA-Tet, HA-Tet (+CM) and HA-TCO (+CM), or CM-HA-Tet and CM-HA-TCO was individually prepared. hPLSCs were added to each of the solutions as follows: 50鈥壩糒 of a suspension of hPLSCs (1鈥壝椻€?06 cells/mL) was individually added to the wells of a 24-well plate and mixed with hPLSC-loaded Cx-HA (1鈥壝椻€?05 cells) [from HA-Tet and HA-TCO], Cx-HA (+CM) (1鈥壝椻€?05 cells) [from HA-Tet (+CM) and HA-TCO (+CM)], or the CM-Cx-HA hydrogel (105 cells) [from CM-modified HA-Tet and CM-modified HA-TCO]. The Cx-HA (+CM) and CM-Cx-HA hydrogels contained the same amounts of CM (0.4鈥塵g/mL, 0.6鈥塵M). The concentration of Cx-HA, 20鈥塵g/mL, was the same as well.The hPLSC-loaded Cx-HA, Cx-HA (+CM), and Cx-HA-CM hydrogels were incubated for 7 days at 37鈥壜癈 and 5% CO2. For comparison, hPLSC-loaded Cx-HA was added to the wells of a 24-well plate by passage through a 26-gauge needle attached to a syringe. All hydrogels were cultivated with replacement of the culture medium every 3 days. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed at 1, 4, and 7 days. For the MTT assay, 100鈥壩糒 of a PBS solution of the MTT substrate (Roche, Basel, Switzerland) was added to each well. After incubation at 37鈥壜癈 for 4鈥塰, the resulting violet formazan precipitate was solubilized by the addition of 500鈥壩糒 of DMSO and shaking for 30鈥塵in. An aliquot (100鈥壩糒) of solution from each well was transferred to a 96-well plate. The solutions were then read on an ELISA plate reader (EL808 Ultra microplate reader, Bio-Tek, Winooski, VT, USA). The optical density at 590鈥塶m of the solution in each well was determined.PKH-labeled hPLSC-loaded Cx-HA, Cx-HA (+CM), and Cx-HA-CM hydrogels were individually prepared with the same concentrations of PKH-labeled hPLSCs, CM, and Cx-HA according to the procedure described in the foregoing subsection. The PKH-labeled hPLSCs on Cx-HA, Cx-HA (+CM), and Cx-HA-CM hydrogels were examined at 1, 7, and 14 days using an Olympus IX51 inverted fluorescence microscope (Olympus, Tokyo, Japan) and analyzed using Motic Images Advanced 3.2 software (Motic Co., Ltd., Hong Kong, China).In vivo imaging of CM-FI鈥搇oaded Cx-HA [Cx-HA (+CM-FI)] and CM-FI鈥搈odified Cx-HA [Cx-HA-CM-FI]Solutions (200鈥壩糒; 4鈥塵g Cx-HA) of [HA-Tet (+CM-FI) and HA-TCO (+CM-FI)] and [HA-Tet-CM-FI and HA-TCO-CM-FI] with CM-FI (124鈥壩糶) were separately drawn into the barrels of a dual-barrel syringe and injected into subcutaneous tissue using a 26-gauge needle.NIR images were obtained using fluorescence excitation at 460鈥塶m and emission light filtered through a 500- to 525-nm bandpass filter with an exposure time of 500鈥塵s and a gain of one using a dichroic cube filter (MgF2, fused silica filter). At each time point, NIR images were captured using an imaging instrument (FOBI, NeoScience, Suwon, Korea).In vivo implantation of hPLSC-loaded Cx-HA, Cx-HA (+CM) and Cx-HA-CMThe protocol used in the animal experiments in this study was approved by the Institutional Animal Experiment Committee (Approval No. 2017-0015) of Ajou University School of Medicine, and all animals used in this work were treated in accordance with the Guidelines for the Care and Use of Animals for Experimental and Scientific Purposes. Male nude mice (20鈥?2鈥塯, 6 weeks old) were used in accordance with the approved guidelines.Each solution (200鈥壩糒; 4鈥塵g Cx-HA) of [HA-Tet and HA-TCO], [HA-Tet (+CM) and HA-TCO (+CM)], and [HA-Tet-CM and HA-TCO-CM] was prepared using the same amount of CM (0.4鈥塵g/mL) and the same number of BrdU-labeled hPLSCs (5鈥壝椻€?06 cells/mL). The solutions were then separately drawn into the compartments of a dual-barrel syringe. A 26-gauge needle attached to the dual syringe was used to inject the solutions into the subcutaneous dorsum of nude mice that had been anesthetized with 60鈥壩糒/kg Zoletil-Rompun (1:1 solution). The resulting BrdU-labeled hPLSC-loaded Cx-HA, BrdU-labeled hPLSCs-Cx-HA (+CM), and BrdU-labeled hPLSCs-Cx-HA-CM hydrogel implants were allowed to develop and were biopsied after 1, 2, 3, and 4 weeks.Histological analysis of implants chondrogenically differentiated in vivoAt 1, 2, 3, or 4 weeks after the injection, the experimental mice were individually euthanized. The implants were collected and fixed in 10% formalin for 7 days. The fixed tissue implants were embedded in paraffin and cut into 4-渭m slices. The paraffinized slides were incubated at 80鈥壜癈 in an oven for 2鈥塰 to bond the tissue to the glass.For hematoxylin and eosin (H E) staining, the paraffinized slides were deparaffinized with xylene and hydrated in a series of 100, 95, 80, 70, and 60% ethyl alcohol solutions. The slides were then washed in running tap water, stained with hematoxylin (Muto Pure Chemicals, Tokyo, Japan) for 3鈥塵in, and washed with DW. The hematoxylin-stained slides were sequentially stained with eosin for 2鈥塵in and washed with DW. The stained slides were hydrated using 95% ethyl alcohol, 100% ethyl alcohol and xylene for 2鈥塵in and were then fixed and mounted in mounting medium (Muto Pure Chemicals, Tokyo, Japan).For Alcian blue staining, the deparaffinized slides were first washed with DW and then treated with an Alcian blue (Sigma, MO, USA) solution for 30鈥塵in. The slides were washed three times with DW. The nuclei of cells in tissues were stained with Nuclear Fast Red (Sigma, MO, USA) and then fixed and mounted using mounting medium.For SO staining, the deparaffinized slides were washed three times with DW and treated with Mayer鈥檚 hematoxylin solution for 5鈥塵in followed by washing with DW for 20鈥塵in. The stained slides were developed in 0.002% Fast Green solution (Sigma-Aldrich, St. Louis, MO, USA) for 30鈥塻 and washed with 1% glacial acetic acid. The slides were then placed in 0.1% SO solution (Sigma-Aldrich, St. Louis, MO, USA) for 6鈥塵in and fixed using mounting medium.For immunofluorescent staining for type 2 collagen, the deparaffinized slides were hydrated for 5鈥塵in and then treated with citrate buffer at 120鈥?30鈥壜癈 for 10鈥塵in. The slides were washed with PBS and PBS-T and blocked with 5% bovine serum albumin (BSA; Millipore, Billerica, MA, USA) and 5% horse serum (HS; Gibco, Auckland, New Zealand) in PBS for 90鈥塵in at 37鈥壜癈. The slide sections were incubated for 16鈥塰 at 4鈥壜癈 with an antibody against collagen type 2 alpha 1 (Abcam, Cambridge, UK) diluted in antibody diluent (DaKo, Glostrup, Denmark) (1:500), washed with PBS and PBS-T, and incubated with a secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor庐 594; Invitrogen, USA) (1:200) for 3鈥塰 at room temperature in the dark. The slides were washed with PBS and PBS-T, counterstained with DAPI in DW (1:1000) for 20鈥塵in, and mounted using fluorescent mounting solution (DaKo, Glostrup, Denmark).For macrophage (ED1) staining, the slides were incubated for 10鈥塵in in citrate buffer at 120鈥?30鈥壜癈; they were then incubated in PBS for 10鈥塵in and washed with PBS containing 0.05% Tween 80 (PBST, Sigma, St. Louis, MO, USA). The slides were blocked in PBS containing horse serum (HS; Gibco, Auckland, New Zealand) and 5% bovine serum albumin (BSA; Roche, Penzberg, Germany) for 90鈥塵in at 37鈥壜癈. The slides were incubated at 4鈥壜癈 with ED1 (mouse anti-rat CD68 antibody; Serotec, Oxford, UK) in antibody diluent (DAKO, Glostrup, Denmark) (1:1000) for 12鈥?6鈥塰, washed with PBS and PBST, and incubated with a secondary antibody (goat anti-mouse Alexa Fluor 594; Invitrogen, CA, USA) (1:200) for 3鈥塰. The slides were washed again with PBST, counterstained with DAPI, and mounted using fluorescent mounting solution (DAKO, Glostrup, Denmark). The slides were visualized using an LSM 710 microscope (Carl Zeiss Microimaging GmbH, G枚ttingen, Germany) and analyzed using ZEN 2009 software (Carl Zeiss Microimaging GmbH, G枚ttingen, Germany).Determination of the GAG and DNA content of in vivo chondrogenically differentiated implantsThe excised hydrogel implants were incubated for 24鈥塰 at 60鈥壜癈 in papain solution (95鈥塵M l-cysteine, 100鈥塵M Na2HPO4, 5鈥塵M EDTA, 125鈥壜礸/mL type III papain, pH 7.5). The resulting solution was centrifuged at 12000鈥壝椻€?i>g for 10鈥塵in. For measurement of the GAG content of implants, 20鈥壜礚 of the upper layer obtained after centrifugation was mixed for 5 seconds with 200鈥壜礚 of DMMB solution [16鈥塵g DMMB (Sigma, MO, USA) in 1鈥塋 of water containing 3.04鈥塯 glycine (Sigma, MO, USA), 1.6鈥塯 NaCl (Sigma, MO, USA), and 95鈥塵L of 0.1鈥塎 acetic acid (Sigma, MO, USA)]. The GAG content was determined using a Cytation 3 (BioTek, VT, USA) at an absorbance of 525鈥塶m. The amount of GAG in implants was calculated through comparison with standard calibration curves prepared using known concentrations of chondroitin sulfate obtained from shark cartilage (Sigma, MO, USA).For measurement of the DNA content of implants, 100鈥壜礚 of the upper layer obtained by centrifugation was reacted for 5鈥塵in with 100鈥壜礚 of PicoGreen reagent (Invitrogen, Carlsbad, CA, USA) in Tris-EDTA buffer (Sigma, MO, USA) (1:200). DNA content was determined based on the intensity of light emission at 520鈥塶m after excitation at 480鈥塶m using a Cytation 3 (BioTek, VT, USA). The amount of DNA in the implants was calculated through comparison with standard calibration curves prepared using known concentrations of a 位 DNA standard (Invitrogen, Carlsbad, CA, USA). GAG content was normalized to DNA content.Gene expression in in vivo chondrogenic differentiated implantsTo examine in vivo RNA expression, the hydrogel implants were excised after 1, 2, 3, and 4 weeks. In vivo RNA expression was determined using a method similar to the method used in the in vitro experiment described above. All samples were analyzed in triplicate.Statistical analysisValues reflecting in vitro and in vivo gene expression, rheological properties, cytotoxicity, and counts of BrdU-, type 2 collagen-, and ED1-positive cells were obtained from three independent experiments. All data are presented as the mean and standard deviation (SD). To evaluate significance, the results were subjected to one-way analysis of variance (ANOVA) with Bonferroni鈥檚 multiple-comparison correction in SPSS 12.0 software (SPSS Inc., Chicago, IL, USA).Results and DiscussionIn vitro chondrogenic differentiation of hPLSCsTo study the stemness and ex vivo expansion of hPLSCs as a cell source for cartilage tissue engineering, hPLSCs isolated from a young woman were allowed to proliferate in culture medium and were expanded up to passage five. The hPLSCs tested positive for MSC markers CD90 ( 99.9%) and CD166 ( 99.8%) and negative for the hematopoietic stem cell marker CD34 ( 0.01%). hPLSCs maintained their stem cell characteristics through five passages, indicating their relative tolerance to ex vivo manipulation. Additionally, hPLSCs showed a higher proliferation rate than did hMSCs and were viable after cryopreservation. Thus, hPLSCs are suitable as a source of stem cells for subsequent experiments on chondrogenic differentiation.Although CM can effectively induce chondrogenic differentiation of hMSCs34,35,36,37, to the best of our knowledge the effect of these peptides on hPLSCs has not been investigated. To examine chondrogenic differentiation of hPLSCs under the influence of CM or TGF-尾, hPLSCs were cultured for 4 weeks in culture medium (control) or in CM or TGF-尾 chondrogenic medium (see the experimental section). Because TGF-尾 is a potent growth factor for chondrogenesis, it was chosen as a positive control in the in vitro experiment. The characteristics of chondrogenic differentiation were identified by H E, SO, and type 2 collagen staining (Fig. 2).Fig. 2a鈥?b>c Staining and (d鈥?b>f) gene expression during the in vitro chondrogenic differentiation of hPLSCs in culture medium (control) or in CM or TGF-尾 chondrogenic medium: a H b SO; c type 2 collagen staining. Expression of the genes (d) SOX9, e ACAN, and f COL2A1 in hPLSCs after 2 and 4 weeks is shown (*p鈥?i> 鈥?.001; **p鈥?i> 鈥?.05)Full size imageEven after 4 weeks of cultivation, hPLSCs in the control group manifested no chondrogenic characteristics. This result indicates that hPLSCs were unable to differentiate in the absence of CM or TGF-尾 as a chondrogenic factor. In contrast, as shown by SO and type 2 collagen staining, hPLSCs cultured in CM or TGF-尾 chondrogenic medium showed chondrogenic characteristics after 2 and 4 weeks. These data confirm that CM induces chondrogenic differentiation of hPLSCs.To further characterize the chondrogenic differentiation, we performed semiquantitative reverse-transcription PCR to measure the mRNA expression of the genes SOX9, ACAN, and COL2A1 (Fig. 2d). 伪-tubulin mRNA expression was used as a reference.After 2 weeks, we observed little expression of SOX9, ACAN, and COL2A1. After 4 weeks, the expression of SOX9, ACAN, and COL2A1 increased in the TGF-尾 and CM groups but not in the control group. The expression levels of SOX9, ACAN, and COL2A1 in the presence of CM or TGF-尾 were ~鈥?3 and 15-fold higher, respectively, than those in the control. At this time point, the SOX9, ACAN, and COL2A1 expression levels in the TGF-尾 group were only slightly higher than those in the CM group.These results provide a positive answer to the first key question posed in this study: CM is almost as effective as TGF-尾 in inducing the chondrogenic differentiation of hPLSCs.Preparation of an injectable HA hydrogel formulationFirst, the carboxylic group in HA was activated by DMTMM. Then, HA-Tet and HA-TCO were individually prepared by a simple reaction between the activated carboxylic groups in HA and the amine groups in Tet-amine and TCO-amine (Supplementary Fig. S1). The characteristic peaks corresponding to Tet and TCO in HA were observed in the 1H-NMR spectra (Supplementary Fig. S2). The yields of HA-Tet and HA-TCO were greater than 90%. Elemental analysis of the amine groups in HA-Tet and HA-TCO showed that there was quantitative introduction of Tet and TCO (Supplementary Table 1).HA-Tet and HA-TCO were dissolved in PBS. As shown in Supplementary Fig. S2, HA-TCO formed a colorless solution; however, due to the original color of Tet, the HA-Tet solution was slightly violet in color. Mixing of equal amounts of the HA-Tet and HA-TCO solutions immediately yielded click-crosslinked HA (Cx-HA). The typical time required for Cx-HA formation was less than 3鈥塻. The 1H-NMR spectrum of Cx-HA revealed new peaks f and g, which appeared after the click reaction between Tet and TCO.In all of the following experiments, we loaded HA-Tet and HA-TCO in equal amounts into the individual compartments of a dual-barrel syringe; Cx-HA formed immediately via mixing at the injection site. These data provide a positive answer to the second key question posed in our study.Characterization of HA and Cx-HA hydrogelsThe mechanical properties of HA and Cx-HA were analyzed in rheological experiments that utilized a change in frequency from 0.1 to 10鈥塇z (Fig. 3). The strain sweep of the two hydrogels was determined from 0.1% to 1000% strain at a frequency of 1鈥塇z; the limiting strain amplitudes of HA and Cx-HA were found to be ~鈥?2.8 and 99.5%, respectively (Fig. 3a). HA and Cx-HA were stable over the tested frequency range of 0.1 to 10鈥塇z, but Cx-HA manifested a higher storage modulus than HA; these data indicate that Cx-HA possesses greater mechanical stiffness than HA.Fig. 3: Rheological characterization of HA and Cx-HA:a the strain sweep test; b viscosity analysis; c storage and loss modulus measurements; d phase angle experiment; e swelling ratio versus time; f imaging of Cx-HA before and after swelling; g SEM image of Cx-HA (*p鈥?lt;鈥?.001)Full size imageThe complex viscosity of Cx-HA was 10-fold higher than that of HA (Fig. 3b). The storage (G鈥? and loss (G鈥? moduli of HA and Cx-HA were measured at 37鈥壜癈 (Fig. 3c). The storage modulus of Cx-HA was found to be higher than that of HA, and the loss modulus of Cx-HA was lower than that of HA. The difference between the storage and loss values of Cx-HA was larger than the difference between the storage and loss values of the HA hydrogel. This result suggests that Cx-HA possesses good elastic characteristics.Next, the elasticity levels of these materials were compared by determining the phase angles (tan 未) of HA and Cx-HA (Fig. 3d). The tan 未 value of HA was 1.2, indicative of predominantly viscous behavior. The tan 未 value of Cx-HA was found to be 0.02, suggesting that the hydrogel strength parameters of Cx-HA are better, i.e., that Cx-HA is significantly stiffer. Collectively, these results indicate that in many respects Cx-HA forms a significantly stiffer hydrogel than HA.Next, the swelling ratios of HA and Cx-HA were compared. HA was completely solubilized in PBS within 15鈥塵in (Fig. 3e). In contrast, Cx-HA maintained the shape of HA but absorbed 2500% of its volume of PBS in 10鈥塵in, 3900% of its volume in 1鈥塰, and 6600% of its volume over a period of 24鈥塰 (Fig. 3f). This result indicates that Cx-HA can rapidly absorb a large volume of biological medium. Furthermore, in SEM, the Cx-HA hydrogel was found to have a porous interconnected three-dimensional structure (Fig. 3g).Next, the mechanical properties of Cx-HA before and after swelling were analyzed (Supplementary Fig. S3). After swelling, Cx-HA exhibited 70% of the complex viscosity exhibited by Cx-HA before swelling. The storage and loss moduli of HA and Cx-HA were measured. The storage and loss moduli of Cx-HA after swelling were found to be slightly lower than those observed before swelling. The difference in the storage and loss moduli of Cx-HA after swelling was still large. This result shows that the elastic characteristics of Cx-HA are maintained after swelling.Collectively, these results support the basic assumption of this study that by permitting the diffusion of biological nutrients, the Cx-HA hydrogel performs well as a three-dimensional scaffold for growth, proliferation, and differentiation of the loaded hPLSCs.In vivo persistence of HA and Cx-HAThe in vivo persistence of the hydrogels was studied because it is important to their function as a durable scaffold for hPLSC-based cartilage tissue engineering. NIR images of NIR fluorescence-labeled HA or Cx-HA were captured to assess the in vivo persistence of HA and Cx-HA hydrogels.First, the prepared NIR-HA-Tet and NIR-HA-TCO solutions were loaded into the compartments of a dual-barrel syringe, as illustrated in Fig. 4a. Mixing of the NIR-HA-Tet and NIR-HA-TCO solutions resulted in the formation of NIR-Cx-HA. The prepared NIR-HA or the NIR-HA-Tet and NIR-HA-TCO solutions were individually subcutaneously injected into nude mice. Immediately after the injection, an NIR-HA or NIR-Cx-HA hydrogel formed at the injection site.Fig. 4a Light microscopy and near-infrared (NIR) images of NIR-Cx-HA and of HA-Tet and HA-TCO formulations in a dual-barrel syringe. NIR images after injection of (b) NIR-HA and (c) NIR-Cx-HA are shown (scale bars鈥?鈥?0鈥塵m)Full size imageNIR fluorescent images of NIR-HA could be captured until 12鈥塰 after injection, but no signals were observed at 1 day after injection (Fig. 4b). In contrast, NIR fluorescent images of the Cx-HA hydrogel could be successfully captured in vivo for at least 40 days (Fig. 4c). This result indicates that the Cx-HA hydrogel persisted in vivo for a much longer period than the HA hydrogel.Collectively, our results corroborate the basic assumption of this study, that is, that for a predefined experimental period, the Cx-HA hydrogel is suitable as a scaffold of hPLSCs.In vitro and in vivo release of CM-FI from physically CM-FI鈥搇oaded Cx-HA [Cx-HA (鈥?鈥塁M-FI)] and from CM-FI鈥搈odified Cx-HA [Cx-HA-CM-FI]We compared the persistence of CM inside Cx-HA after the physical and chemical loading of CM into the hydrogel. First, to monitor the in vitro degradation of CM-FI, a solution of free CM-FI was incubated at 37鈥壜癈 for 28 days, and its fluorescence intensity was then determined. The fluorescence intensity of CM-FI did not change markedly over the 28-day period, indicating that little or no degradation occurred for at least 28 days under our in vitro experimental conditions (Supplementary Fig. S4).Next, the in vitro cumulative release of CM-FI from Cx-HA (+CM-FI) and Cx-HA-CM-FI was studied (Fig. 5a, b). From Cx-HA (+CM-FI), CM-FI showed 44% release at 5鈥塵in, 83% release at 12鈥塰, and 92% release at 1 day, indicating an initial burst of release at 1 day. This shows that physically loaded CM-FI is almost completely liberated from Cx-HA after only 1 day.Fig. 5a, b In vitro and (c) in vivo CM-FI release from Cx-HA (+CM-FI) and Cx-HA-CM-FI hydrogels (scale bars鈥?鈥?0鈥塵m). Data are presented for (a) a 28-day period and (b) for a shorter time period of 24鈥塰Full size imageThe release of CM-FI from Cx-HA-CM-FI showed the following pattern: 1.3% of the fluorescence intensity was released after 1 day, 2.4% was released after 7 days, and 6.3% was released at 28 days, indicating that, compared with Cx-HA (+CM-FI), only a slow release of CM-FI occurs. This result indicated that chemical attachment of CM to the Cx-HA hydrogel prolonged the in vitro residence period of CM inside the Cx-HA hydrogel.To examine the in vivo persistence of CM-FI release by real-time imaging, Cx-HA (+CM-FI) and Cx-HA-CM-FI were injected (separately) into the subcutaneous tissue of mice (Fig. 5c). The injected Cx-HA (+CM-FI) and Cx-HA-CM-FI emitted green fluorescent signals at the initial time point of 30鈥塵in. The fluorescence intensity of Cx-HA (+CM-FI) gradually decreased over a 4-day period and was almost lost by day 4; only negligible fluorescence was detectable on day 10. In the mouse that received an injection of Cx-HA-CM-FI, fluorescent signals were observed at 30鈥塵in, and they persisted for 28 days after the injection.These results support the basic assumption of this study, namely, that chemical attachment of CM to Cx-HA results in persistent in vitro and in vivo CM release from the Cx-HA hydrogel for a predefined experimental period.In vitro and in vivo viability of hPLSCs in Cx-HA, Cx-HA (+CM), and Cx-HA-CM hydrogelsAlthough HA is widely known as a biocompatible biomaterial13, we measured the viability of PKH-labeled hPLSCs in vitro. Cx-HA, Cx-HA (+CM), and Cx-HA-CM containing PKH-labeled hPLSCs were loaded into multiwell plates to evaluate their biocompatibility over a 7-day period (Supplementary Fig. S5). At 1 day, similar numbers of PKH-labeled hPLSCs were observed on Cx-HA, Cx-HA (+CM), and Cx-HA-CM hydrogels. The PKH-labeled hPLSCs proliferated for 7 days in all the Cx-HA鈥揵ased hydrogels. The number of PKH-labeled hPLSCs on the hydrogels did not differ significantly among the groups. This finding suggests that all of the Cx-HA鈥揵ased hydrogels are biocompatible with hPLSCs.Next, the in vitro viability of hPLSCs after passage of the cells through syringe needles during the injection process was examined. There were slight differences in the viability of the cells before and after passage through the needle; however, the hPLSCs showed high viability (80鈥?2%), comparable to that of hPLSCs before passage through the needle (Supplementary Fig. S6). We confirmed that the injectable click-crosslinked formulation prepared in this work may act as a cell carrier when the cells are forcibly passed through the syringe needles, although the viability of the hPLSCs was slightly affected by the injection method40,41.Next, to examine the in vivo viability of hPLSCs in the injectable hydrogel, injectable Cx-HA, Cx-HA (+CM), and Cx-HA-CM, together with BrdU-labeled hPLSCs, were injected subcutaneously into nude mice. Immediately after injection, all of the formulations formed Cx-HA hydrogel鈥揵ased scaffolds that maintained their shape over the entire 4-week experimental period; there were no signs of inflammation at the injection site. Thin fibrous capsules containing fibroblasts and blood vessels were visible around the Cx-HA hydrogel (Supplementary Fig. S7).BrdU-labeled hPLSCs (red) and nucleic acid-stained hPLSCs (DAPI, blue) in the Cx-HA hydrogel in vivo were examined by fluorescence microscopy at 1, 2, 3, and 4 weeks after injection, and the cells were counted (Supplementary Fig. S8). The intensity of the red signal assignable to BrdU-labeled hPLSCs in the images was similar in samples of the Cx-HA, Cx-HA (+CM), and Cx-HA-CM hydrogels. The number of BrdU-labeled hPLSCs did not differ significantly among the groups. These results show that hPLSCs survived well in vivo in the Cx-HA hydrogel for 4 weeks due to the excellent biocompatibility of the Cx-HA hydrogel.It is well known that macrophage ED1 expression is a unique in vivo indicator of the inflammatory response of host tissues to implants42. Accordingly, to examine the inflammatory response of the host tissue to Cx-HA, Cx-HA (+CM), and Cx-HA-CM containing hPLSCs, all of the hydrogel implants were evaluated by immunohistochemical ED1 staining of macrophages (Supplementary Fig. S9). Relative to the number of nuclei stained with DAPI (blue), very few ED1 (red)-stained macrophages were observed among the ED1-postive cells. This result indicates that Cx-HA is highly biocompatible.Histological characteristics of implants chondrogenically differentiated in vivoTo examine the in vivo chondrogenic differentiation of hPLSCs in Cx-HA鈥揵ased hydrogels, each injectable formulation (Cx-HA, Cx-HA (+CM), and Cx-HA-CM) was subcutaneously injected with and without hPLSCs into nude mice. At 1, 2, 3 and 4 weeks after the injection, samples of the injected material were stained with H E (Supplementary Fig. S10). H E-stained histological sections of implants of Cx-HA, Cx-HA (+CM), and Cx-HA-CM without hPLSCs showed little or no tissue organization. In contrast, implants of Cx-HA, Cx-HA (+CM), and Cx-HA-CM with hPLSCs displayed a more organized tissue-like structure.The implants of Cx-HA, Cx-HA (+CM), and Cx-HA-CM with or without hPLSCs were subjected to Alcian blue (AB) and SO staining to confirm the presence of GAG (Fig. 6 and Supplementary Fig. S11). The blue images in group AB and the brown images in group SO were attributed to the presence of a cartilaginous matrix of GAG. The red images in group AB and the gray images in group SO were attributed to the nucleic acids present in the aggregated hPLSCs.Fig. 6a Alcian blue (AB) and (b) SO-stained histological sections of implants of Cx-HA, Cx-HA (+CM), and Cx-HA-CM without (鈭? and with (+) hPLSCs. The implants were excised and analyzed after 1, 2, 3, or 4 weeks (magnification 脳800; scale bars represent 25鈥壩糾)Full size imageOnly slight AB and SO staining was observed in Cx-HA, Cx-HA (+CM), and Cx-HA-CM implants without hPLSCs, even after 4 weeks. In contrast, an organized cartilaginous matrix and aggregated hPLSCs were found in implants of scaffolds Cx-HA, Cx-HA (+CM), and Cx-HA-CM containing hPLSCs. The Cx-HA-CM hydrogel with hPLSCs contained the most organized cartilaginous matrix and contained aggregated hPLSCs. Especially after 2 weeks, Cx-HA-CM with hPLSCs showed some rounded morphology that appeared to be due to the presence of chondrocytic lacunae. Chondrocytic lacunae surrounded by a deposition of GAG (brown color) were clearly visible, mostly after 3 and 4 weeks. These results suggest that chemical attachment of CM to the Cx-HA enhanced the chondrogenic differentiation of hPLSCs to a greater extent than Cx-HA (+CM).Collectively, these results support the main hypothesis that constitutes the third key question of this study; i.e., they show that CM that remains inside Cx-HA for a long time can synergistically induce the chondrogenic differentiation of hPLSCs for cartilage tissue engineering.Expression of type II collagen and GAG content of implants chondrogenically differentiated in vivoGiven that type 2 collagen can be expressed in chondrogenically differentiated implants as the dominant extracellular matrix protein in a cartilaginous matrix43, the expression of type 2 collagen was analyzed by immunohistochemical staining of Cx-HA, Cx-HA (+CM), and Cx-HA-CM implants containing hPLSCs after 1, 2, 3, or 4 weeks (Fig. 7). The type 2 collagen鈥損ositive cells present in the hydrogels were identified and counted.Fig. 7: Histological sections of Cx-HA, Cx-HA (+CM), and Cx-HA-CM implants after immunofluorescent staining for type 2 collagen.hPLSCs were removed after 1, 2, 3, or 4 weeks (magnification 200脳; scale bars represent 100鈥壩糾) (*p鈥?lt;鈥?.001; **p鈥?lt;鈥?.05)Full size imageCx-HA with hPLSCs showed only weak positive staining (violet color), but greater positive staining for type 2 collagen was observed after 4 weeks, probably due to in vivo chondrogenesis by hPLSCs under the action of the HA scaffold itself26. In contrast, positive staining of Cx-HA (+CM) with hPLSCs was barely detectable at 1 week but increased sharply at 2 weeks and then remained almost unchanged for 4 weeks. The reason for this is probably that CM was released from Cx-HA for 10 days, consistent with the results of the experiments with CM-FI described in a previous subsection; thus, CM was not able to induce the chondrogenic differentiation of hPLSCs during an extended period.Although Cx-HA-CM with hPLSCs exhibited weak positive staining for type 2 collagen after 1 week, Cx-HA-CM with hPLSCs showed stronger positive staining than either Cx-HA or Cx-HA (+CM). After 1 week, the positive staining increased gradually and rapidly as a function of post-implantation time. This result suggests that due to the action of the CM that remained inside the Cx-HA for an extended period, hPLSCs differentiated into cartilage-like tissues, resulting in the abundant synthesis of type 2 collagen.GAGs are important components of cartilage, of the synovial fluid that lubricates joints, and of connective tissues and tendons44. Consequently, the GAG content of the implants of Cx-HA, Cx-HA (+CM), and Cx-HA-CM with hPLSCs was quantified after 1, 2, 3, and 4 weeks (Fig. 8). At 1 week, all of the implants showed low GAG content, and the GAG content did not differ significantly among the groups. Small amounts of GAGs in the Cx-HA and Cx-HA (+CM) hydrogels showed almost no variation in GAG content, although they increased with time post-implantation. In contrast, the GAG content of implants of Cx-HA-CM with hPLSCs was clearly higher than that of the implants in the Cx-HA and Cx-HA (+CM) with hPLSCs groups. The GAG content of Cx-HA-CM with hPLSCs increased gradually over the 4-week period; in addition, there was a significant difference in GAG content between Cx-HA (+CM) and Cx-HA-CM after 3 and 4 weeks (p鈥?lt;鈥?.05 and 0.001, respectively). These data suggest that chondrogenic differentiation of hPLSCs inside the Cx-HA hydrogel induced the formation of a larger amount of GAGs due to the retention of CM in the hydrogel for a long time. The high concentration of GAGs in the Cx-HA-CM implants is in good agreement with the results of AB and SO staining described in a preceding subsection.Fig. 8: GAG concentration in Cx-HA, Cx-HA (+CM), and Cx-HA-CM hydrogels containing hPLSCs.The hydrogel implants were removed after 1, 2, 3, or 4 weeks (*p鈥?lt;鈥?.001; **p鈥?lt;鈥?.05)Full size imageGene expression in implants chondrogenically differentiated in vivoThe formation of cartilage-like tissues in in vivo chondrogenically differentiated implants was examined by semiquantitative PCR analysis of SOX9, ACAN, and COL2A1 expression at 1, 2, 3, and 4 weeks after in vivo injection (Fig. 9). The in vivo gene expression patterns were almost identical to the in vitro patterns described in a previous subsection. After 1, 2, 3, and 4 weeks, the mRNA expression of SOX9, ACAN, and COL2A1 in the Cx-HA-CM hydrogel containing hPLSCs was clearly higher than that in Cx-HA and Cx-HA (+CM) hydrogels with hPLSCs.Fig. 9a SOX9, (b) ACAN, and (c) COL2A1 mRNA expression levels in Cx-HA, Cx-HA (+CM), and Cx-HA-CM implants containing hPLSCs. The implants were excised after 1, 2, 3, or 4 weeks (*p鈥?lt;鈥?.001)Full size imageAt 3 weeks, the mRNA expression of SOX9, an early marker of the formation of a cartilage-like tissue matrix45,46, in the Cx-HA-CM hydrogel with hPLSCs was clearly higher than its expression at 4 weeks. The mRNA levels of ACAN and COL2A1, two markers of the final stage of chondrogenic differentiation42, increased gradually as a function of time in the Cx-HA-CM hydrogel containing hPLSCs and showed significantly higher expression after 4 weeks (p鈥?lt;鈥?.001). This result indicates that the chondrogenic differentiation of hPLSCs inside the Cx-HA hydrogel induces the formation of cartilage-like tissues by CM that is retained within the hydrogel for a long time.Collectively, these results support a positive answer to our third key question, namely, that in comparison to induction with CM physically loaded inside Cx-HA, the hPLSC-loaded Cx-HA-CM hydrogel induces better chondrogenic differentiation of hPLSCs under the action of the retained CM.ConclusionIn this work, we confirmed that an in situ鈥揻orming Cx-HA-CM hydrogel and hPLSCs have good potential for cartilage tissue engineering. The CM that is retained inside the Cx-HA hydrogel for a long time synergistically induces the chondrogenic differentiation of hPLSCs. Although the exact mechanism of CM action was not determined in this work, we confirmed that the action of CM remaining inside the Cx-HA for an extended period on cell surface TGF-尾 receptors can synergistically induce the chondrogenic differentiation of hPLSCs for cartilage tissue engineering. The injectable formulation of the Cx-HA-CM鈥揵ased hydrogel described in this work provides an opportunity to satisfy the unmet need for (pre)clinical repair of damaged articular cartilage. References1.Vilela, C. A. et al. Cartilage repair using hydrogels: a critical review of in vivo experimental designs. ACS Biomater. Sci. Eng. 1, 726鈥?39 (2015).CAS聽Google Scholar聽 2.Szychlinska, M. A. et al. Mesenchymal stem cell-based cartilageregeneration approach and cell senescence: Can we manipulate cell aging and function? Tissue Eng. Part B Rev. 23, 529鈥?39 (2017). Google Scholar聽 3.Chu, C. R., Szczodry, M. Bruno, S. Animal models for cartilage regeneration and repair. Tissue Eng. Part B Rev. 16, 105鈥?15 (2010). Google Scholar聽 4.Feng, Q. et al. One-pot solvent exchange preparation of non-swellable, thermoplastic, stretchable and adhesive supramolecular hydrogels based on dual synergistic physical crosslinking. NPG Asia Mat. 10, e455 (2018). Google Scholar聽 5.Makris, E. A., Gomoll, A. H., Malizos, K. N., Hu, J. C. Athanasiou, K. A. Repair and tissue engineering techniques for articular cartilage. Nat. Rev. Rheuma. 11, 21鈥?4 (2015).CAS聽Google Scholar聽 6.Wang, Z. et al. Novel biomaterial strategies for controlled growth factor delivery for biomedical applications. NPG Asia Mat. 9, e435 (2017).CAS聽Google Scholar聽 7.Chou, C. L. et al. Micrometer scale guidance of mesenchymal stem cells to form structurally oriented large-scale tissue engineered cartilage. Acta Biomater. 60, 210鈥?19 (2017).CAS聽Google Scholar聽 8.Irawan, V., Sung, T., Higuchi, A. Ikoma, T. Collagen scaffolds in cartilage tissue engineering and relevant approaches for future development. Tissue Eng. Reg. Med 15, 673鈥?97 (2018).CAS聽Google Scholar聽 9.Wang, J., Zhang, F., Tsang, W. P., Wan, C. Wu, C. Fabrication of injectable high strength hydrogel based on 4-arm star PEG for cartilage tissue engineering. Biomaterials 120, 11鈥?1 (2017).CAS聽Google Scholar聽 10.Gay, I. C., Chen, S. MacDougall, M. Isolation and characterization of multipotent human periodontal ligament stem cells. Orthod. Craniofacial Res 10, 149鈥?60 (2007).CAS聽Google Scholar聽 11.Charles Huang, C. Y., Pelaez, D., Bendala, J. D., Garcia-Godoy, F. Cheung, H. S. Plasticity of stem cells derived from adult periodontal ligament. Regen. Med. 4, 809鈥?21 (2009). Google Scholar聽 12.Um, S. et al. Valproic Acid Modulates the multipotency in periodontal ligament stem cells via p53-mediated cell cycle. Tissue Eng. Reg. Med. 14, 153鈥?62 (2017).CAS聽Google Scholar聽 13.Collins, M. N. Birkinshaw, C. Hyaluronic acid based scaffolds for tissue engineering鈥擜 review. Carbohydr. Polym. 92, 1262鈥?279 (2013).CAS聽Google Scholar聽 14.Liang, X., Fang, L., Li, X., Zhang, X. Wang, F. Activatable near infrared dye conjugated hyaluronic acid based nanoparticles as a targeted theranostic agent for enhanced fluorescence/CT/photoacoustic imaging guided photothermal therapy. Biomaterials 132, 72鈥?4 (2017).CAS聽Google Scholar聽 15.Kim, S. H., In, I. Park, S. Y. pH-Responsive NIR-absorbing fluorescent polydopamine with hyaluronic acid for dual targeting and synergistic effects of photothermal and chemotherapy. Biomacromolecules 18, 1825鈥?835 (2017).CAS聽Google Scholar聽 16.Hwang, D. W. et al. In vivo visualization of endogenous miR-21 using hyaluronic acid-coated graphene oxide for targeted cancer therapy. Biomaterials 121, 144鈥?54 (2017).CAS聽Google Scholar聽 17.Raia, N. R. et al. Enzymatically crosslinked silk-hyaluronic acid hydrogels. Biomaterials 131, 58鈥?7 (2017).CAS聽Google Scholar聽 18.Qu, X., Qiu, P., Zhu, Y., Yang, M. Mao, C. Guiding nanomaterials to tumors for breast cancer precision medicine: from tumor-targeting small-molecule discovery to targeted nanodrug delivery. NPG Asia Mat. 9, e452 (2017).CAS聽Google Scholar聽 19.Unterman, S. A. et al. Hyaluronic acid-binding scaffold for articular cartilage repair. Tissue Eng. Part A 18, 2497鈥?506 (2012).CAS聽Google Scholar聽 20.Kim, I. L., Khetan, S., Baker, B. M., Chen, C. S. Burdick, J. A. Fibrous hyaluronic acid hydrogels that direct MSC chondrogenesis through mechanical and adhesive cues. Biomaterials 34, 5571鈥?580 (2013).CAS聽Google Scholar聽 21.Zhu, D., Wang, H., Trinh, P., Heilshorn, S. C. Yang, F. Elastin-like protein-hyaluronic acid (ELP-HA) hydrogels with decoupled mechanical and biochemical cues for cartilage regeneration. Biomaterials 127, 132鈥?40 (2017).CAS聽Google Scholar聽 22.Yang, J., Zhang, Y. S., Yue, K. Khademhosseini, A. Cell-laden hydrogels for osteochondral and cartilage tissue engineering. Acta Biomater. 57, 1鈥?5 (2017).CAS聽Google Scholar聽 23.Yu, F. et al. An injectable hyaluronic acid/PEG hydrogel for cartilage tissue engineering formed by integrating enzymatic crosslinking and Diels鈥揂lder 鈥渃lick chemistry鈥? Polym. Chem. 5, 1082鈥?090 (2014).CAS聽Google Scholar聽 24.Liu, J. et al. Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration. NPG Asia Mat. 10, 827鈥?39 (2018).CAS聽Google Scholar聽 25.Ren, K., He, C., Xiao, C., Li, G. Chen, X. Injectable glycopolypeptide hydrogels as biomimetic scaffolds for cartilage tissue engineering. Biomaterials 51, 238鈥?49 (2015).CAS聽Google Scholar聽 26.Toh, W. S., Lim, T. C., Kurisawa, M. Spector, M. Modulation of mesenchymal stem cell chondrogenesis in a tunable hyaluronic acid hydrogel microenvironment. Biomaterials 33, 3835鈥?845 (2012).CAS聽Google Scholar聽 27.Yoon, I. S. et al. Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in porous hyaluronic acid scaffold. J. Biosci. Bioeng. 112, 402鈥?08 (2011).CAS聽Google Scholar聽 28.Devaraj, N. K., Upadhyay, R., Haun, J. B., Hilderbrand, S. A. Weissleder, R. Fast and sensitive pretargeted labeling of cancer cells through a tetrazine/trans-cyclooctene cycloaddition. Angew. Chem. Int. Ed. Engl. 48, 7013鈥?016 (2009).CAS聽Google Scholar聽 29.Li, Z. et al. Tetrazine鈥?i>trans-cyclooctene ligation for the rapid construction of 18F labeled probes. Chem. Commun. 46, 8043鈥?045 (2010).CAS聽Google Scholar聽 30.Liu, D. S. et al. Diels鈥揂lder cycloaddition for fluorophore targeting to specific proteins inside living cells. J. Am. Chem. Soc. 134, 792鈥?95 (2012).CAS聽Google Scholar聽 31.Gopinathan, J. Noh, I. Click Chemistry-based injectable hydrogels and bioprinting inks for tissue engineering applications. Tissue Eng. Reg. Med 15, 531鈥?46 (2018).CAS聽Google Scholar聽 32.Owen, S. C., Fisher, S. A., Tam, R. Y., Nimmo, C. M. Shoichet, M. S. Hyaluronic acid click hydrogels emulate the extracellular matrix. Langmuir 29, 7393鈥?400 (2013).CAS聽Google Scholar聽 33.Weiss, A. Attisano, L. The TGFbeta superfamily signaling pathway. Wiley Interdiscip. Rev. Dev. Biol. 2, 47鈥?3 (2013).CAS聽Google Scholar聽 34.Lam, H. J., Li, S., Lou, N., Chu, J. Bhatnagar, R. S. Synthetic peptides cytomodulin-1 (CM-1) and cytomodulin-2 (CM-2) promote collagen synthesis and wound healing in vitro. Conf. Proc. IEEE Eng. Med. Biol. Soc 7, 5028鈥?030 (2004). Google Scholar聽 35.Renner, J. N. Liu, J. C. Investigating the effect of peptide agonists on the chondrogenic differentiation of human mesenchymal stem cells using design of experiments. Biotechnol. Prog. 29, 1550鈥?557 (2013).CAS聽Google Scholar聽 36.O鈥橩ane, S. Ferguson, M. W. J. Transforming growth factors-b and wound healing. Int. J. Biochem. Cell Biol. 29, 63鈥?8 (1997). Google Scholar聽 37.Mittal, A., Kumar, R., Parsad, D. Kumar, N. Cytomodulin-functionalized porous PLGA particulate scaffolds respond better to cell migration, actin production and wound healing in rodent model. J. Tissue Eng. Regen. Med. 8, 351鈥?63 (2014).CAS聽Google Scholar聽 38.Park, S. H. et al. BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells. Sci. Rep. 7, 6603 (2017). Google Scholar聽 39.Hyun, H. et al. Central C鈥揅 bonding increases optical and chemical stability of NIR fluorophores. RSC Adv. 4, 58762鈥?8768 (2014).CAS聽Google Scholar聽 40.Cohen, D. L. et al. Increased mixing improves hydrogel homogeneity and quality of three-dimensional printed constructs. Tissue Eng. Part C. 17, 240鈥?48 (2011). Google Scholar聽 41.Wang, L. L. et al. Three-dimensional extrusion bioprinting of single- and double-network hydrogels containing dynamic covalent crosslinks. J. Biomed. Mater. Res. A 106, 865鈥?87 (2018).CAS聽Google Scholar聽 42.Setzu, A. et al. Inflammation stimulates myelination by transplanted oligodendrocyte precursor cells. Glia 54, 297鈥?03 (2006). Google Scholar聽 43.Monibi, F. A. Cook, J. L. Tissue-derived extracellular matrix bioscaffolds: emerging applications in cartilage and meniscus repair. Tissue Eng. Part B Rev. 23, 386鈥?98 (2017).CAS聽Google Scholar聽 44.Lin, Y. et al. Molecular and cellular characterization during chondrogenic differentiation of adipose tissue鈥恉erived stromal cells in vitro and cartilage formation in vivo. J. Cell. Mol. Med. 9, 929鈥?39 (2005).CAS聽Google Scholar聽 45.Kawaguchi, J., Mee, P. J. Smith, A. G. Osteogenic and chondrogenic differentiation of embryonic stem cells in response to specific growth factors. Bone 36, 758鈥?69 (2005).CAS聽Google Scholar聽 46.Kronenberg, H. M. Developmental regulation of the growth plate. Nature 423, 332鈥?36 (2003).CAS聽Google Scholar聽 Download referencesAcknowledgementsThis study was supported by a grant from the Basic Science Research Program (2016R1A2B3007448) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education and by a grant from the Korea Health Technology R D Project (HI17C2191) through the Korea Health Industry Development Institute funded by the Ministry of Health Welfare.Author informationAffiliationsDepartment of Molecular Science and Technology, Ajou University, Suwon, 16499, KoreaSeung Hun Park,聽Ji Young Seo,聽Joon Yeong Park,聽Yun Bae Ji,聽Kyungsook Kim,聽Sangdun Choi,聽Jae Ho Kim,聽Byoung Hyun Min聽 聽Moon Suk KimGordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USAHak Soo ChoiAuthorsSeung Hun ParkView author publicationsYou can also search for this author in PubMed聽Google ScholarJi Young SeoView author publicationsYou can also search for this author in PubMed聽Google ScholarJoon Yeong ParkView author publicationsYou can also search for this author in PubMed聽Google ScholarYun Bae JiView author publicationsYou can also search for this author in PubMed聽Google ScholarKyungsook KimView author publicationsYou can also search for this author in PubMed聽Google ScholarHak Soo ChoiView author publicationsYou can also search for this author in PubMed聽Google ScholarSangdun ChoiView author publicationsYou can also search for this author in PubMed聽Google ScholarJae Ho KimView author publicationsYou can also search for this author in PubMed聽Google ScholarByoung Hyun MinView author publicationsYou can also search for this author in PubMed聽Google ScholarMoon Suk KimView author publicationsYou can also search for this author in PubMed聽Google ScholarContributionsS.H.P., J.Y.S., J.Y.P., Y.B.J., K.K., and J.H.Y. performed the experiments on hyaluronic acid hydrogel preparation, cell viability and CM release. H.S.C. prepared and discussed ZW800-1C. S.H.P., J.Y.S., and Y.B.J. conducted the animal experiments. S.H.P., J.Y.P, K.K., J.H.K., S.C., and M.S.K. prepared and discussed the stained images. M.S.K. designed all of the experiments and wrote the paper. All of the authors discussed the results and commented on the manuscript.Corresponding authorCorrespondence to Moon Suk Kim.Ethics declarations Conflict of interest The authors declare that they have no conflict of interest. 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To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Reprints and PermissionsAbout this articleCite this articlePark, S.H., Seo, J.Y., Park, J.Y. et al. An injectable, click-crosslinked, cytomodulin-modified hyaluronic acid hydrogel for cartilage tissue engineering. NPG Asia Mater 11, 30 (2019). https://doi.org/10.1038/s41427-019-0130-1Download citationReceived: 17 January 2019Revised: 26 March 2019Accepted: 31 March 2019Published: 21 June 2019DOI: https://doi.org/10.1038/s41427-019-0130-1 Editorial SummaryTissue engineering: an injectable hydrogel for repairing damaged jointsInjectable polymers that spontaneously form three-dimensional porous scaffolds may make it easier to repair cartilage tissue in arthritis sufferers. Recent findings have shown that periodontal stem cells harvested from tissue surrounding human teeth can differentiate into cartilage cells when seeded onto scaffolds containing bioactive peptides. Moon Suk Kim from Ajou University in Suwon, South Korea, and colleagues have now improved this approach by chemically attaching peptides that mimic natural growth factors to water-filled polymers called hydrogels. Cell culture experiments demonstrated that this new material improved resistance against breakdown compared with hydrogels containing physically absorbed bioactive peptides. By injecting live mice with injectable polymers, the team induced hydrogels via click-crosslink into a subcutaneous scaffold loaded with periodontal stem cells. Hydrogels revealed the formation of cartilagelike tissues over four weeks without causing inflammation.

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