Description:

BacPAK6Sec+linearizedbaculovirusDNAisthelatestbaculovirusexpressionsystemtocomeoutoftheOETlabs.BytakingtheoriginalBacPAK6systemandgeneticallymodifyingit,BacPAK6Sec+isabletoincreasetheyieldofrecombinantproteinandtheeaseofharvesting.

BacPAK6Sec+containsadeletionofthechitinasegene(chiA)fromtheoriginalBacPAK6viralbackbone.

Chitinasehasbeenshownbybothconfocalandelectronmicroscopytolocatetotheendoplasmicreticulum,whereitisdenselypackedinaparacrystallinearray,whichseverelyinhibitsthefunctionandefficacyofthesecretorypathway.BydeletingthechiA,secretionoftargetproteinsintothecytoplasmisincreased,greatlyenhancingtheyieldofharvestablerecombinantprotein.

TheconceptofdeletingthechiAgenecameafterthesuccessoftheflashBAC™platformthatutilizesthesamegeneticdeletionforincreasedrecombinantproteinyield.TofindoutmoreabouttheflashBAC™system,clickhere.

BacPAK6linearDNAistheoriginalconvenient,highlyefficientreagentforgeneratingrecombinantbaculovirusexpressionvectors. Itcomprisesamodified Autographacalifornica nucleopolyhedrovirus(AcMNPV)genomewith lacZ insertedinplaceofthenativepolyhedringenecodingregionforconvenientblue/whiteselection.

DigestionofBacPAK6DNAatthreesiteswithBsu36Iremoves lacZ andpartofORF1629,whichisanessentialAcMNPVgene. ThelinearvirusDNAisunabletoinitiateareplicationcycleininsectcells.
Co-transfectionofinsectcellswithlinearBacPAK6DNAandacompatIBLetransfervectorcontainingthecompleteORF1629andaforeigngeneofinterestresultsinrecombinationandrestorationofthecircular,infectiousvirusgenome. Over95%ofthevirusderivedfromtheco-transfectioncomprisesrecombinantvirus. Usually,asingleplaqueassaytitrationincubatedwithX-galandcounterstainedwithneutralredissufficienttodifferentiateparentalbluefromrecombinantwhite(colourless)plaques. ThesecanbereADIlyisolatedandamplifiedtoworkingstocksofpurifiedrecombinantvirusinafewdays.

TheBacPAK6Sec+reagentfromOETLtdcomprisestransfection-ready,linearized(Bsu36I-digested)virusDNA. Itisavailablein5,24and96reactionpacks. Forbulkpurchases,pleasecontactusoninfo@oetltd.com.

WeofferapromotionaldealonallBacPAK6productstoincludeafree3reactionflashBAC™kittohelpyoudecideifmakingtheswitchistherightchoiceforyourlab.

Oxford-baculoQUANT™多合一病毒提取和滴定试剂盒£ 405.00 不含增值税 增值税产品描述baculo QUANT™ALL-IN-ONE（Cat＃100602）是OET与安捷伦科技合作开发的具有革命性的基于qPCR的杆状病毒DNA提取和定量试剂盒。准确的病毒滴度对于确定正确的感染复数和确保最佳的重组蛋白生产至关重要。传统上，杆状病毒是通过噬斑测定或基于抗体的测定进行滴定，需要3-4天，但是杆状QUANT™ALL-IN-ONE试剂盒包含用于提取病毒DNA并在数小时内确定准确滴度的试剂。使用baculo QUANT™ALL-IN-ONE的好处：滴度准确度可与噬斑测定法媲美不到一小时的动手时间，不到三小时即可完成病毒DNA提取和滴定与任何包含gp64病毒基因的基于AcMNPV的杆状病毒系统兼容单步病毒DNA提取无需柱qPCR引物/探针作为单一试剂混合物提供安捷伦科技的高性能，超灵敏的预混试剂斑块滴定滴定的病毒储备用于标准曲线生成100个反应试剂盒可通过3条标准曲线一式三份进行多达24次滴定