Lucigen品牌DB0715K/Baseline-ZERO™DNase价格货期/厂家直采
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Lucigen公司介绍美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™AK文库构建试剂盒、DNATerminator®末端修复试剂盒、EconoTaq®DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖Lucigen品牌DB0715K/Baseline-ZERO™DNase价格货期/厂家直采
Lucigen品牌DB0715K/Baseline-ZERO™DNase价格货期/厂家直采
| ProductDescription | Size | Cat.No. | Quantity | BUY | ||
| Baseline-ZERO™DNase | 5,000MBU | DB0715K | 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 |
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Baseline-ZERO™DNase
EffectivelydigestlargeorsmalldsDNAandssDNAintomononucleotides
![\"Epicentre](\"https://www.ebiomall.com/images/image/9321558076133.png\")
DigestunwanteddsDNAandssDNAmoleculesincludingsmallssDNAsuchasrandomhexamers
UseinsensitiveapplicationsrequiringreversetranscriptionwhereanycontaminatingDNAisunwanted
Applications RemovalofgenomicDNAfromsmall-sampletotalRNApreparationsforexpressionanalysis(Fig.1) RemovalofsmallDNAoligonucleotides(e.g.,randomprimers) Baseline-ZERO™DNase*digestsdsDNAandssDNAintomononucleotidesmoreeffectivelythanthecommonlyusedbovinepancreaticDNaseI.EventhesmallDNAoligonucleotidesthatremainaftertreatmentwithbovinepancreaticDNaseIareundetectablebygelelectrophoresisfollowingtreatmentwithBaseline-ZERODNase(Fig.2).RemovalofDNAfromRNApreparationsisparticularlybeneficialwhenRNAinasampleisamplifiedusingamethodthatinvolvesreversetranscriptionusingrandomprimers,sinceanycontaminatingDNAwouldalsobeatemplateforrandom-primedCDNAsynthesis. |
Figure1.Real-timePCRofHeLaRNApreparationstreatedwithvariousDNases. ThelowertheCT value(intersectionofcurveswiththeredline),thegreatertheamountofresidualDNAnotdigestedbytheindicatedDNase.Thus,Baseline-ZERO™DNaseremovedalldetectableDNAfromtheRNAsample.TheTaqMan®probeassayamplifieda268-bpfragmentofβ-actin.Sampleswereruninduplicate. |
![\"\"](\"https://www.lucigen.com/images/Epicentre/c_baselinezero_helarnapreparations.gif?sfvrsn=0.2358954647707565\" "\\"c_BaselineZero_HeLaRNAPreparations\\"")
UnitDefinition: OneMolecularBIOLOGyUnit(MBU)ofBaseline-ZERO™DNaseproducesanincreaseintheA260 ofasolutionofdsDNA,of0.001perminuteat25°C.Functionally,1MBUcompletelydigests1µgoflinearpUC19DNAtomononucleotidesin10minutesat37°C. StorageBuffer: Baseline-ZERODNaseissuppliedina50%glycerolsolutioncontaining50mMTris-HCl(pH7.5),10mMCaCl2,10mMMgCl2 and0.1%Triton®X-100. 10XBaseline-ZERO™DNaseReactionBuffer: 100mMTrisHCl(pH7.5),25mMMgCl2 and5mMCaCl2. 10XBaseline-ZERO™DNaseStopSolution: 30mMEDTA. QualityControl: Baseline-ZERODNaseisassayedforitsABIlitytocompletelydigestlineardsDNAtomononucleotidesunderstandardassayconditions.Baseline-ZERODNaseisfreeofdetectableRNaseactivitiesasassayedbyPAGEanalysisof1µgofasyntheticRNAtranscriptfollowinganovernightincubationwithsufficientDNaseItocompletelydigest1,000 µgofDNA. |
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