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Lucigen/T4 DNA Polymerase, Exonuclease Minus/30085-3/1500 U

价格
¥9340.00
货号:30085-3
浏览量:127
品牌:Lucigen
服务
全国联保
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商品描述
  • OnlysourceforrecombinantT4DNAPolymerase,ExonucleaseMinus.
  • PotentialapplicationsincludeenhancingDNAsequencingreads.

T4DNAPolymerase,ExonucleaseMinusisaDNA-dependentDNApolymerasewhichcatalyzesthe5’→3’synthesisofDNAandrequirestemplateandprimer. Additionally,theenzymelacks3’→5’exonucleaseactivity.

Source:
PurifiedfromastrainofE.colithatcarriesaT4DNAPolymerase,ExonucleaseMinusexpressionplasmid.

Applications:

  • DNASequencingreaction.(1)

Heatinactivation:70°Cfor15minutes.

Storage:
Enzymeandbuffershouldbestoredat-20°C.Enzymeisstablefor12monthsifhandledproperly.

AdditionalInformation:

  • MayincreasetheDNAsequencingread-lengths.

References

  1. NancyG.Nossal(1998)Genetics148:1535-1538.

ORDERINFORMATION

RecombinantT4DNAPolymerase,ExonucleaseMinusissuppliedataconcentrationof3,000U/mlinastoragebufferof50%glycerol,100mMKPO4(pH6.5)and1mMDTT.Alsosuppliedis10XDNAPolymeraseBufferAcomposedof100mMTris-HCl(pH7.9),100mMMgCl2,500mMNaCland10mMDTT. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。