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- OnlysourceforrecombinantT4DNAPolymerase,ExonucleaseMinus.
- PotentialapplicationsincludeenhancingDNAsequencingreads.
T4DNAPolymerase,ExonucleaseMinusisaDNA-dependentDNApolymerasewhichcatalyzesthe5’→3’synthesisofDNAandrequirestemplateandprimer. Additionally,theenzymelacks3’→5’exonucleaseactivity.
Source:
PurifiedfromastrainofE.colithatcarriesaT4DNAPolymerase,ExonucleaseMinusexpressionplasmid.
Applications:
- DNASequencingreaction.(1)
Heatinactivation:70°Cfor15minutes.
Storage:
Enzymeandbuffershouldbestoredat-20°C.Enzymeisstablefor12monthsifhandledproperly.
AdditionalInformation:
- MayincreasetheDNAsequencingread-lengths.
References
- NancyG.Nossal(1998)Genetics148:1535-1538.