

Excellentstranddisplacement,fidelityandspeed.
phi29DNAPolymeraseisahighlyprocessiveDNApolymerasewithapowerfulstranddisplacementactivityanda3′→5′proofreADIngexonucleasefunction.Theenzymeiscapableofupto70,000insertionsperbindingevent.
![]() |
Anexampleofthesavingsyoucanexpect. |
Specifications
UnitDefinition:1unitisdefinedastheamountofpolymeraserequiredtoconvert0.5pmolofdNTP"sintoacidinsolublematerialin10minutesat30°C.
Source:ArecombinantE.colistraincarryingthephi29DNAPolymerasegenefrombacteriophagephi29.
UnitConcentration | 10,000U/mL |
Purity(SDS-PAGE) | >99% |
SSExonuclease | Functional |
E.coli16SrDNAContamination | 100U<10copies |
Endonuclease | 100U<10%converted |
Storage | -20&°C |
ORDERINFORMATION
Suppliedat10,000U/mLin10mMTris-HCl,100mMKCl,0.1mMEDTA,1mMdithiothreitol,0.5%Tween-20,0.5%NP-40,50%glycerol,pH7.4@25°C.Alsosuppliedis10Xphi29DNAPolymeraseBuffercomposedof500mMTris-HCl,100mM(NH4)2SO4,40mMDithiothreitol,100mMMgCl2,pH7.5@25°C.10,000Uissuppliedas5×2,000U.
Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。