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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/Bst DNA Polymerase, Exonuclease Minus 8,000 U/ml/30027-2/10,000 (5 x 2,000 Units)

Lucigen/Bst DNA Polymerase, Exonuclease Minus 8,000 U/ml/30027-2/10,000 (5 x 2,000 Units)

价格
￥6340.00
货号：30027-2
浏览量：127
品牌：Lucigen
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商品描述
  • Highstranddisplacementactivity.
  • UsedinisothermalamplificationandNextGenerationSequencing.
FreeSampleAvailable

HighspecificactivityforDNAamplificationandsequencing.

Applications

  • Stranddisplacementamplification*
  • DNAsequencingthroughhighGCregions(1,2)
  • RapidsequencingfromnanogramamountsofDNAtemplate(3)

BstDNAPolymerase,ExonucleaseMinus,isarecombinantformofthe67kDaBacillusstearoThermophilusDNAPolymeraseprotein(largefragment).Theenzymehas5’-3’polymeraseactivityandstranddisplacementactivity,butitlacks3’-5’exonucleaseactivity.Italsohasreversetranscriptionactivity.

Lucigen'sBstDNAPolymerase,ExonucleaseMinus,hashigherstranddisplacementactivitythanthatofothersuppliers(Figure1).Theenzymecanbeusedinnucleicacidamplificationmethods*suchasisothermalamplification,wholegenomeamplification(WGA),andmultipledisplacementamplification(MDA).ItalsocanbeusedinNextGenerationsequencing.

Thisenzymehasoptimalactivityat65°C.ItissuitableforsequencingDNAwithhighGCcontentandsecondarystructures.Itisavailableinconcentrationsof8,000U/mlor50,000U/ml.

BstDNAPolymerase,ExonucleaseMinus,issuppliedwith10XDNAPolymeraseBufferB,composedof200mMTris-HClpH8.8,100mM(NH4)2SO4,100mMKCl,20mMMgSO4,and1.0%TritonX-100.

Lucigen Bst versus competitors.

Figure1.LucigenBstDNAPolymerase,ExonucleaseMinus,possessesgreaterstrand-displacingpolymeraseactivity.M13singlestrandedDNAwasincubatedwithorwithout8unitsofBstDNAPolymerase(+/-Bst)inreactionbuffersuppliedbythemanufacturer,withorwithoutreplicationprimer(+/-primer)for30minutesat65°C.MW,1kbladder.

Tipsforuse:

  • Requires0.1%TritonX-100forlongtermstorage.
  • Reactiontemperaturesabove70°Carenotrecommended.
  • BstDNAPolymerasecannotbeusedforthermalcyclesequencing.

HeatInactivation:80ºCfor20min.

References:

  1. Griffin,H.andGriffin,A.(1994)PCRTechnology,228-229.
  2. McClary,J.etal.(1991)J.DNASequencingandMapping,1,173-180.
  3. Mead,D.A.etal.(1991)Biotechniques,11,76-87.


*Note:
Someusesforthisproductmayrequirelicenses.Lucigendoesnotencourageorsupporttheunauthorizedorunlicenseduseofpatentednucleicacidamplificationprocessesforisothermalamplification,wholegenomeamplification(WGA),multipledisplacementamplification(MDA),andNextGenerationsequencing.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.Ifthepurchaserisnotwillingtoaccepttheseuselimitations,LucigenCorporationiswillingtoacceptreturnoftheproductforafullrefund.

ORDERINFORMATION

BstDNAPolymerase,ExonucleaseMinusisavailableintwoconcentrations,8,000U/mland50,000U/ml,inastoragebufferof10mM Tris-HCl,pH7.5,50 mM KCl,1 mM DTT,0.1 mM EDTA,0.1% TritonX-100,and50% Glycerol.Alsosuppliedis10XDNAPolymeraseBufferB,composedof200 mM Tris-HClpH8.8,100 mM (NH4)2SO4,100 mM KCl,20 mM MgSO4, and1.0 % TritonX-100. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。