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Lucigen/SelecTEV™ Protease/30810-1/1,000 U

价格
¥2620.00
货号:30810-1
浏览量:127
品牌:Lucigen
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商品描述
  • Highspecificactivityandspecificity
  • EngineeredforstABIlity
  • 6-Histidinetagforeasyremoval

SelecTEV™Proteaseisa27kDimprovedformofTobaccoEtchVirus(TEV)proteasethathasbeenengineeredtobemorestable,active,andspecificthanthenativeprotease.SelecTEV™ProteaseisacysteineproteasethatrecognizestheTEVrecognitionsequenceGlu-Asn-Leu-Tyr-Phe-Gln-GlyandcleavesbetweenGlnandGly.Thisseven-amino-acidsequenceisrarelyfoundinproteins,makingSelecTEV™Proteaseanidealchoicefortagremovaloffusionproteins.Theoptimaltemperatureforcleavageis30°C,howevertheenzymeisactiveoverawidetemperaturerange(4–30°C)andpH(6.0–8.5)toaccommodateyourspecificprotein.Followingdigestion,SelecTEV™ProteaseiseasilyremovedfromthecleavagereactionbyaffinitychromatographyusingthepolyhistidinetagattheN-terminusoftheprotease.SelecTEV™ProteaseispurifiedfromE.colibyaffinitychromatographyusingthepolyhistidinetagandis90%purewhenvisualizedonanSDS-PAGEgel.

SelecTEVProteasecanbeusedwiththeExpressoSolubilityandExpressionScreeningSystemvectors,whichcontainaTEVcleavagerecognitionsite.

SelecTEV Protease

Coomassie-stainedSDS-PAGEgelofTEVcleavagereaction.10UofSelecTEVProteasewasincubatedwith30µgoffusionproteinsubstrateat30°Cfor1hourina100µLreaction.Marker(laneM),SelecTEVProtease(Lane1),Uncleavedfusionsubstrate(Lane2),CleavedsubstrateandSelecTEVProtease.(Lane3)

ORDERINFORMATION

30810-1:SelecTEVProtease(1tube),100mMDTT(1tube),20XSelecTEVBuffer(1tube)

30810-2:SelecTEVProtease(5tubes),100mMDTT(5tubes),20XSelecTEVBuffer(5tubes)

Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。