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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit/MBCL90310/10 Reactions

Lucigen/MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit/MBCL90310/10 Reactions

价格
￥4520.00
货号：MBCL90310
浏览量：127
品牌：Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述

ProduceamplifiedamountsofCDNAfromprecious,limitingcellsampleswithoutintroducingbias.

  • PerformRNAamplificationdirectlyfromculturedcelllysatesfollowedbycDNAsynthesisFormerly from Epicentre
  • Amplifywiththishigh-fidelity,linearRNAamplificationprocessthatpreservestherelativetranscriptabundanceofthesample.
  • Getmoredataoutofprecioussamples–uselessRNAformoreRT-qPCRreactions
  • ReADIlyandreproducIBLydetectevenlow-abundancetranscriptsfromaslittleasonecell.
  • NoneedtopurifyRNA:PerformRNAamplificationandcDNAsynthesisreactionsdirectlyfromcelllysates.
  • CollectsampleslessoftenandarchivecDNAforfutureuse,savingtimeandeffort.

Applications

  • AmplificationofmRNAdirectlyfromacelllysate,withouttheneedforpurifyingRNA,andconversionoftheamplifiedRNAtocDNA.
  • SignificantlyincreasingthenumberandsensitivityofqRT-PCRsfromverysmallnumbersofcells.
  • GenerationoflargeamountsofcDNAfromthemRNAofverysmallsamplesforarchivalpurposes.

TheMessageBOOSTER™cDNASynthesisfromCellLysatesKitenablestheusertoperformsensitiveqRT-PCRstudiesfromverysmallpopulationsofcells,evenfromaslittleasonecell.

ThekitamplifiesthecellularmRNAdirectlyfromacelllysate,withouttheneedtopurifytotalRNA,andthenconvertstheamplifiedRNAtocDNAthatisreadyforqPCR(Fig.1).AmplifiedcDNAisefficientlyproducedfromthelysatesof1to500cells.

Figure1(clicktoenlarge).AnoverviewoftheprocedurefortheMessageBOOSTER™cDNASynthesisfromCellLysatesKit.Akitreactionamplifiesthepoly(A)RNA(mRNA)directlyfromacrudecelllysatewithouttheneedforRNApurification.TheamplifiedRNAisthenreverse-transcribedtocDNAthatcanbedilutedupto1,000-foldforqPCR.
Figure2.AMessageBOOSTER™KitreactionproducessufficientcDNAfromasingle-celllysateforthousandsofsensitiveqPCRs.qPCRwasperformedusingundiluted(red),1:10diluted(green),1:100diluted(blue),and1:1,000diluted(purple)cDNAfromalysateofasingleNRKcell.Thelow-abundancePBGDtranscriptwasreadilydetected.

ORDERINFORMATION

Contents:QuickExtract™RNAExtractionSolution,MessageBOOSTER™T7-Oligo(dT)Primer,MessageBOOSTER™ReverseTranscriptionPreMix,RNaseInhibitor,MMLVReverseTranscriptase,MessageBOOSTER™DNAPolymerasePreMix,MessageBOOSTER™DNAPolymerase,MessageBOOSTER™cDNAFinishingSolution,MessageBOOSTER™InVitroTranscriptionPreMix,MessageBOOSTER™T7RNAPolymerase,MessageBOOSTER™T7TranscriptionBuffer,RNase-FreeDNaseI,RandomPrimers,RNaseH,ForwardControlPCRPrimer,ReverseControlPCRPrimer,100mMDTT,RNase-FreeWater,Poly(I). Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。