Quicklypurifyhighyieldsofhigh-molecular-weightgenomicDNA,totalcellularRNAorTotalNucleicAcid(TNA)withonekit
Fast:PurifyTotalNucleicAcid(TNA),DNAorRNAin30–60minutes- Safe:Doesnotusehazardousphenol,chloroformorguanidine
- HighPurity:A260/A280ratiosconsistentlybetween1.8and2.0
- HighYields:Improvesyieldsbyavoidingtheuseofcolumnswhichoftenreducenucleicacidyields
- Versatile:PurifyTNA,genomicDNA,totalRNA,FFPERNA,orbothgenomicDNAandtotalRNAfromasample
- TotalRNARecovery:Purifybothlargeandsmall(e.g.,miRNA)RNAforRNA-SeqorqRT-PCR
- Proven:HundredsofcitationsforpurificationofDNAandRNAfromdozensofsampletypesforuseinmanyapplications
TheMasterPure™CompleteDNAandRNAPurificationKitenablesrapid,highyieldpurificationofhigh-molecular-weightgenomicDNA,totalcellularRNAandTotalNucleicAcid(TNA)frommanydifferentsampletypes.
Thekitprocedureusesasafe,gentle,andscalablesalt-precipitationprotocol(Figure1)thateliminatestheneedforhazardouschemicalsandyield-reducingcolumns.Purificationreactionscanbescaleduporscaleddowndependingontheamountofnucleicacidneededandthesizeofthesample.MasterPureKitsforNucleicAcidpurificationsGram-positivebacteria,yeastDNAandbloodsamplesarealsoavailable.
Applications
PurificationofgenomicDNAortotalRNAformanyapplications,including:
- Librarypreparationfornextgenerationsequencing(NGS)ofgenomicDNAandRNA
- DNAmethylationstudiesusingIllumina®Infinium®HumanMethylationBeadChips
- GenomicDNAandCDNAcloning
- qPCRandqRT-PCR
- Micrarrayanalyses(CGH,geneexpressionprofiling,etc)
Figure1.OverviewoftheMasterPure™CompleteKitprotocol. |
Figure2.ComparisonofPCRsensitivityusingtemplatesobtainedusingtheMasterPure™CompleteDNAandRNAPurificationKitversusaspin-columnkit.PCRamplificationwasperformedafterextractionfromserialdilutionsofE.colicellsrangingfrom2×107(lanes1)to200(lanes6).LaneM,DNAMarker;lane7,negativecontrol. |
Figure3.DNA,RNA,andtotalnucleicacid(TNA)purifiedfromdiversecellsourcesusingtheMasterPure™CompleteKit.M=kbladder. |
