快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/Fast-Link™ DNA Ligation Kit/LK6201H/100 Ligations

Lucigen/Fast-Link™ DNA Ligation Kit/LK6201H/100 Ligations

价格
￥5000.00
货号：LK6201H
浏览量：127
品牌：Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述
  • ClonewithspeedandefficiencyFormerly from Epicentre
  • TransformintoE.coliwithoutclean-upordesalting

Applications

  • Blunt-endandTAcloningofPCRproducts.
  • Ligationofnext-gensequencingadaptorstoblunt-endedDNA.
  • GenomicDNAcloningandsubcloning.
  • BAC/fosmidlibraryconstruction.
  • CDNAcloning.
  • Linkerligation.
TheFast-Link™DNALigationKitusesahigh-qualityligase(Fast-LinkT4DNALigase),thatwasclonedatEpicentreandformulatedtoprovideextremelyrapid,high-efficiencyDNAligation.Cohesive-endligationscanbeperformedin5minutesatroomtemperature.Incontrasttootherligases,itisnotnecessarytodesaltFast-Linkligationreactionspriortotransformationofelectrocompetentorchemicallycompetentcells.TheFast-LinkKitcanbeusedforroutineandhigh-throughputDNAcloning.
TypeofEndLigationTime%WhiteColoniesRecombinantsperµgDNA
Overhang5min.932.0x106
Blunt15min.714.4x105
PCRproductwith3´-Aoverhang1hr.681.2x104

Table1.Fast-Link™representativeresults.

Benefits

  • Cohesive-endligationsin5minutesatroomtemperature(Fig.1).
  • Blunt-endligationsin15minutesatroomtemperature(Fig.2).
  • LigationofPCRproductwithA-overhangsin1hourat16°C.
  • Highligationefficiency(Table1).
  • Desaltingofligationproductsisnotneededpriortotransformationofelectrocompetentorchemicallycompetentcells.
  • BACligationwithinserts>100kbcanbeperformedin4hours,insteadofovernightreactionsaswithotherligases.
Figure1.TimecourseforcohesiveendligationusingtheFast-Link™Kit.LamBDaHindIIIMarkerswereligatedusing2unitsofFast-LinkDNALigase(Lanes2-6).LaneM,Kilobaseladder;lane1,noenzyme.Figure2.Timecourseforblunt-endligationusingtheFast-Link™Kit.pUC19DNAdigestedwithPvuIIwasligatedusing2unitsofFast-LinkDNALigase(lanes1-6).LanesM,Kilobaseladder.

ORDERINFORMATION

Contents:Fast-Link™DNALigase,Fast-Link™10XLigationBuffer,10mMATP. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。