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Lucigen/CloneSmart LCKan Blunt Cloning Kit/40821-4/40 rxns

价格
¥10600.00
货号:40821-4
浏览量:127
品牌:Lucigen
服务
全国联保
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商品描述
  • Convenient,pre-processedvectorseliminatetheneedfordigestion,gelpurification,anddephosphorylation.
  • TranscriptionterminatorsflankingcloningsitestABIlizeotherwisetoxicinserts.
  • Choiceofcopynumberandantibioticresistance.
  • OptimizedforusewithE.cloni®10Gelectrocompetentandchemicallycompetentcells(soldseparately)

Clonequicklyandfacilitatedownstreamapplications

Lucigen’suniquecloningkitswithpre-processedvectorsandhighlycompetentcellseliminatehoursoftediouspreparationandenableefficientcloning.Ligationcanbecompletedinaslittleas30minuteswithnoclean-upneeded;onlyabriefheatdenaturationisnecessarybeforetransformation.Downstreammanipulation(e.g,mutagenesis,subsequentinsertaddition)isfacilitatedbytheminimalvectorsize(1.7-2.0kb).

StabilizedInsertsandGap-FreeCloning

Allcloningkitsincludethetranscription-andtranslation-freepSMART®vectors(Figure1),whicheliminatemanyoftheproblemsassociatedwithcloningrecalcitrantDNAinconventionalplasmidvectors.Conventionalcloningvectorscontainpromoters(e.g.,lacZpromoter)thatconstitutivelytranscribetheinsertsequence.Thistranscriptionandcoupledtranslationcandestabilizeinsertsthatcontaincodingregions,strongpromoters,shortrepeats,orincompatIBLesecondarystructures.ThecloningsiteofpSMARTvectorsdoesnotincludealaczsequence.Inaddition,strongtranscriptionterminatorsflankthecloningsitetoblockspurioustranscriptionfromthevectorandinsert-driventranscriptionintothevector.LowcopynumberversionsofpSMARTfurtherstabilizesequencesthataredifficulttomaintainintypicalvectors.

NoBackgroundProblems

ThepSMARTvectorsaresuppliedpre-digested,withblunt,dephosphorylatedends,andarequalifiedtoproduce99.5%recombinantclonesintypicalexperiments.Theultra-lowbackgroundofemptyvector(lessthan0.5%)eliminatestheneedtoscreenforrecombinants,removestheuncertaintyoffalsenegatives(lightbluepUCcolonies)andfalsepositives(whitecoloniesthatlackinserts),andenableslibraryconstructionfromnanogramamountsofDNA.

Diagram of pSMART vectors.

Figure1.Highcopy(HC)andlowcopy(LC)versionsofthepSMARTtranscription-freecloningvectors.

ConvenientSuccess

EfficientlyobtainyourcloneorcreateyourgenomicorCDNAlibrary—theCloneSmartCloningKitseliminatetediouspreparationofvectorsandcompetentcells,aswellastime-consumingQCtestingexperiments.Kitsincludeoptimizedreagents,ligation-readyvector(nopost-ligationcleanupsteprequired),detailedinstructions,andtrouble-shootingguidestosimplifycloningandsequencing.HighlyefficientE.cloni®10Gelectrocompetentcells(upto>4×1010cfu/µg)orchemicallycompetentcells(>1×109cfu/µg)areavailableforsaleseparately.

ORDERINFORMATION

CloneSmartBluntCloningKitscontain:VectorPremix,CloneSmartDNALigase,PositiveControlInsertDNA,SequencingPrimers,andacompleteprotocol.KitscanbeusedwithanyE.colichemicallycompetentorelectrocompetentcellsbuthavebeenoptimizedforusewithofE.cloni®ElectrocompetentCellsandChemicallyCompetentCells. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。