Lucigen'sCopyRight®v2.0BACcloningkitswithpSMART®BAC(Figure1)orpEZBAC(Figure2)vectorandBAC-OptimizedReplicator™v2.0ElectrocompetentCellsresultinunprecedentedaccuracy,reliability,andefficiencyinBACcloningandlibraryconstruction.ThesevectorsincorporatetheCloneSmart®transcription-freetechnology,whichminimizescloningbiasandenablescloningofotherwisetoxicgenes,alongwithinduciblecopynumberamplification(20-50X)forhighDNAyieldsandalacZ-sacBstufferfragmentthateliminatesuncutvectorfromrecombinants,resultinginzerobackground. UnlikeotherBACcloningsystems,Lucigen'sCloneSmarttechnologyassuresthateven"unclonable"insertsarestablymaintained.Sequencesarenotlostorrearrangedinthecloningprocess. CopyRightKitsoffersuperiorperformanceforBACcloningatamuchbetterpricethanotherkits. Figure3showsanexampleofaRandomShearBAClibrarygeneratedwithpSMARTBAC.
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Figure3.ArabidopsisRandomShearBACLibraryinthepSMARTBACvector.7,680cloneswereobtained,withanaverageinsertsizeof105kband~5Xgenomecoverage. |
ConvenientSuccess
Efficientlyobtainyourcloneorcreateyourlibrary—theCopyRightv2.0CloningKitseliminatetediousvectorandcompetentcellpreparation,aswellastime-consumingQCexperiments. Kitsincludeoptimizedreagents,ligation-readyvector,highlyefficientelectrocompetentcells,detailedinstructions,andatrouble-shootingguide.
ORDERINFORMATION
EachCopyRight®v2.0BACCloningKitcontains:pre-cutvector(pSMARTBACwithachoiceofBamHIorHindIIIsites),CopyRightDNALigase,CopyRight5XLigationBuffer,PositiveControlInsertDNA,SequencingPrimers,BAC-OptimizedReplicatorv2.0ElectrocompetentCellsinDUOpackaging(2transformationspertube),PositiveControlInsertDNA,RecoveryMedium,ArabinoseInductionSolution,andcompleteprotocols.EachCopyRightpEZBACCloningKitcontains:pre-cutCopyRightvector(pEZBACBlunt),CloneSmartDNALigase,CloneDirect™10XLigationBuffer(includesATP),PositiveControlInsertDNA,ReplicatorInductionSolution,SequencingPrimers,andcompleteprotocols.KitswithcellsalsoincludeBAC-OptimizedReplicatorv2.0ElectrocompetentCells(>1×1010 cfu/µgDNA)packagedintwotransformationspertube(DUOs),PositiveControlPlasmid,andRecoveryMedium.
Pleasenotethatthefollowingvectorshavebeendiscontinued:pSMARTBACEcoRI(catalognumbers42031-1,42031-2)andpEZBACBamHI(catalognumbers42007-1,42007-2and42012-1).”
Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。
