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Lucigen/CJ236 Electrocompetent Cells/60701-1/12 rxns

价格
¥3460.00
货号:60701-1
浏览量:127
品牌:Lucigen
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商品描述

Highefficiencycellstocreateuracil-containingDNAforsite-directedmutagenesis

  • SolesourceofhighlyefficientElectrocompetentcells(1×109cfu/µg)
  • ung-anddut-mutationstogenerateDNAwithsomeuracilresidues
  • IdealgenotypeandefficiencyforKunkeldirectedmutagenesismethod1.

1KunkelT.A.1985.Rapidandefficientsite-specificmutagenesiswithoutphenotypicselection. Proc.Natl.Acad.Sci.52:488-492.

Lucigenisthesoleproviderofhigh-efficiencyelectrocompetentCJ236cells.ThiscelllinewascreatedbyaddingthepCJ105plasmidtoBW313cells2,andisideallysuitedtotheKunkeldirectedmutagenesismethod.ThismethodreliesonthecellsproducingplasmidDNAcontainingsomeuracilresidues.Thedut-andung-mutationspreventthecellsfromremovinguracilfromDNAcausedbymisincorporationordCTPdeamination.NotethatthesecellscontainanF’whichconfersresistancetoChloramphenicolatlowlevels.

AsElectrocompetentcells,thetransformationefficiencyis1×109cfu/µg.

Functionalgenotype:
[F’Tra+Pil+(CamR)]ung-1relA1dut-1thi-1spoT1mcrA

2Joyce,C.M.,N.Grindley1984.MethodfordeterminingwhetherageneofEscherichiacoliisessential:applicationtothepolAgene.J.Bacteriol.158:636-643

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。