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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/BigEasy-TSA Electrocompetent Cells/60224-1/6 rxns (SOLOs)

Lucigen/BigEasy-TSA Electrocompetent Cells/60224-1/6 rxns (SOLOs)

价格
￥3360.00
货号：60224-1
浏览量：127
品牌：Lucigen
服务
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商品描述
  • CellsrequiredfortransformationandpropagationofpJAZZ®Vectors.
  • Highestavailabletransformationefficienciesforlibraryconstructionandscreening.
  • OptimizedforhighyieldsofDNA.

BigEasy™-TSA™ElectrocompetentCells(>2×1010cfu/µgpKanRDNA)areforusewithLucigen’spJAZZ®-OCorOKLinearVectorsintheBigEasyv2.0LinearCloningSystem.TheTSACellshavethetelNgenerequiredforhighefficiencytransformation,andtheantAgenerequiredforcopynumbercontrolwiththepJAZZvector,integratedintothehostgenome.ThesecellsalsogivehighyieldandhighqualityplasmidDNAduetotheendA1andrecA1mutations.Theycontainthemcrandmrrmutations,allowingmethylatedgenomicDNAthathasbeenisolateddirectlyfrommammalianorplantcellstobeclonedwithoutdeletionsorrearrangements.

Table1.E.cloniCompetentCellsforBAC,LargeorDifficultFragmentCloning


E.cloniCellLines

TransformationEfficiency
(cfu/µgpUCDNA)

CloningMethylatedDNA

BAC,CosmidCloning

Blue/WhiteScreening

BigEasy-TSAElectrocompetent

>2×1010

YES

No

YES
IPTGinductionrequired

BigEasy-TSA:F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74endA1recA1araD139Δ(ara,leu)7697galUgalKrpsLnupG&lamBDa;-tonAAmpRsopABtelNantA

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasSOLOs(1transformationpertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。