- Cellsthatenablethemostchallenginglibraryconstructionandscreening.
- Highestavailabletransformationefficienciesforlibraryconstructionandscreening.
- OptimizedforhighyieldsofDNA.
LucigenofferstwobacterialstrainsdesignedforBAClibraryconstructionortransformationoflargeconstructs:
BAC-OptimizedReplicator™v2.0ElectrocompetentCells(>1×1010cfu/µgpKanRDNA). Thesecellsarerequiredforon-demand,copynumberamplificationofthepSMART®BACorpEZ™BACvectorsintheCopyRight®Kits,therebyincreasingDNAyields. BAC-OptimizedReplicatorCellsarecomponentsofLucigen’sCopyRight®CloningKits.
E.cloni10GBAC-OptimizedElectrocompetentCells(>1×107cfu/µg150kbBACDNA;>1×1010cfu/µgpUCDNA).ThefirstcompetentcellsdevelopedbyLucigenspecificallyforlargeDNAcloning. Manufacturedusinganovelprocedurethatmaximizestransformationefficiencieswithverylargeinserts,10GBAC-OptimizedCellsareanexcellentchoiceforBAClibraryconstructionandlargeconstructtransformation.
Table1.E.cloniCompetentCellsforBACorLargeFragmentCloning
E.cloniCellLines | TransformationEfficiency | CloningMethylatedDNA | BAC,CosmidCloning | Blue/WhiteScreening |
10GBAC-OptimizedElectrocompetent | >1×1010 | YES | YES | YES |
BAC-OptimizedReplicatorv2.0Electrocompetent | >1×1010 | YES | YES | YES |
ORDERINFORMATION
CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube)orFIVERs(5transformationspertube),asindicated.RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.
Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。
