Clonerepetitivesequencesandlentivirallibraries
- StABIlizedirectrepeatsandcreatelentiviralconstructs
- GeneratelentiviralguideRNAlibraries
- RecommendedinCRISPRGeCKOlibraryprotocols
- Chooseelectrocompetentorchemicallycompetentcells
- Highestefficiencycommerciallyavailablecellsforlentiviralcloning: over1×107cfu/µg(chem)or1×1010cfu/µg(electro)
TransformationofEnduraElectrocompetentCellswithGeCKOlentivirallibrariesmayrequireextraRecoveryMedium. OrderextraRecoveryMediumbelow.
FreeSampleAvailable
Conserveclonesthatcontainunstablesequences!
MaintainunstableDNA.Lucigen’sEnduraCompetentCellsareacommonlyusedstrainforcloningsequencesthatsufferunwantedrecombinationeventsinotherstrains.Cloneswithinvertedrepeatsorothersequencespronetorecombinationarecommonlyfoundinretroviralgenes,andrequirecellssuchasEnduratobepropagatedstably.
Excellentvalueandefficiency!SwitchtoEnduracellstodayandexperienceahigherlevelofefficiencyandasavingsinyourcloningbudget.
ChemicalorElectrocompetent.Whichevermethodoftransformationyouprefer,Lucigencangiveyoubetterefficiencyand/orbetterprices.
Figure1.Costperreactionofchemicallycompetentcellsofclone-stabilizingstrainsfromothersuppliers.
Figure2.Transformationefficienciesofelectrocompetentclone-stabilizingstrains.
ORDERINFORMATION
Endura™CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedas SOLOs (1transformationpertube)orDUOs (2transformationspertube)asindicated.EnduraElectrocompetentcellsareprovidedinDUOsformatonly.RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.
PLEASENOTE: Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells. PleasecontactLucigen.
NOTE: TransformationofEnduraElectrocompetentCellswithGeCKOlentivirallibrariesmayrequireextraRecoveryMedium. TheZhanglabprotocol(availablefromAddgene)recommends~2mLofRecoveryMediumpertransformation. LucigenEndurakitsprovide1mLofRecoveryMediumpertransformation. OrderextraRecoveryMediumaboveorvisittheRecoveryMediumproductpagehere!
Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。
