2. 在多克隆位点区域，拥有5个单一的限制性酶切位点：BamH I，Snab I，EcoR I，Avr II和Not I。3.胞内表达。4.载体构建时，需要包含一个ATG启动子，同时需要加入Kozak序列。 (Cavener and Stuart, 1991; Kozak, 1987; Kozak, 1990)5. 毕赤酵母中使用HIS4基因筛选。6. 将基因插入GS115和KM71的AOX1中，需要使用Sac I线性化质粒(GS115产生的基因型为His+ Mut+，KM71酵母产生的基因型为His+ MutS)。7. 将基因插入GS115和KM71的HIS4中，需要使用Sal I线性化质粒(GS115产生的基因型为His+ Mut+，KM71酵母产生的基因型为His+ MutS)。8. 将基因插入GS115的AOX1基因中，也可以使用Bgl II 限制酶线性化质粒，产生的酵母基因型为His+ MutS。 ORGANISM other sequences; artificial sequences; vectors.COMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT VNTAUTHORNAME|biofeng.com|FEATURES Location/Qualifiers source 1..9004 /organism= pPIC3.5K/mol_type= other DNAmisc_feature complement(1055..1075) /label= 3_AOX1_primerCDS complement(1708..4242) /label= ORF frame 2/translation= MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI*gene complement(4656..5471) /label= KanR2/gene= KanR2/translation= MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI*CDS complement(4656..5471) /label= ORF frame 3/translation= MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*misc_feature complement(5636..5655) /label= pBRrevBam_primer/translation= MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*CDS complement(5637..6359) /label= ORF frame 3/translation= MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT PKQALMSPKWRARSSPSVMSAI*misc_feature complement(6641..6663) /label= pGEX_3_primer/translation= MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT PKQALMSPKWRARSSPSVMSAI*rep_origin complement(7060..7679) /label= pBR322_origin/translation= MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT PKQALMSPKWRARSSPSVMSAI*gene complement(7834..8694) /label= Ampicillin/gene= Ampicillin/translation= MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT PKQALMSPKWRARSSPSVMSAI*CDS complement(7834..8694) /label= ORF frame 2/translation= MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*promoter complement(8736..8764) /label= AmpR_promoter/translation= MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW* ORIGIN 1 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG 61 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT 121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC 181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA 241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA 301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG 361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT 421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG 481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT 541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT 601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT 661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT 721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT 781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT 841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA 901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCTACGTAG AATTCCCTAG 961 GGCGGCCGCG AATTAATTCG CCTTAGACAT GACTGTTCCT CAGTTCAAGT TGGGCACTTA 1021 CGAGAAGACC GGTCTTGCTA GATTCTAATC AAGAGGATGT CAGAATGCCA TTTGCCTGAG 1081 AGATGCAGGC TTCATTTTTG ATACTTTTTT ATTTGTAACC TATATAGTAT AGGATTTTTT 1141 TTGTCATTTT GTTTCTTCTC GTACGAGCTT GCTCCTGATC AGCCTATCTC GCAGCTGATG 1201 AATATCTTGT GGTAGGGGTT TGGGAAAATC ATTCGAGTTT GATGTTTTTC TTGGTATTTC 1261 CCACTCCTCT TCAGAGTACA GAAGATTAAG TGAGAAGTTC GTTTGTGCAA GCTTATCGAT 1321 AAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG 1381 AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA 1441 GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATA TCGTCCATTC CGACAGCATC 1501 GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC 1561 GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT 1621 GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CTATCGAATC 1681 TAAATGTAAG TTAAAATCTC TAAATAATTA AATAAGTCCC AGTTTCTCCA TACGAACCTT 1741 AACAGCATTG CGGTGAGCAT CTAGACCTTC AACAGCAGCC AGATCCATCA CTGCTTGGCC 1801 AATATGTTTC AGTCCCTCAG GAGTTACGTC TTGTGAAGTG ATGAACTTCT GGAAGGTTGC 1861 AGTGTTAACT CCGCTGTATT GACGGGCATA TCCGTACGTT GGCAAAGTGT GGTTGGTACC 1921 GGAGGAGTAA TCTCCACAAC TCTCTGGAGA GTAGGCACCA ACAAACACAG ATCCAGCGTG 1981 TTGTACTTGA TCAACATAAG AAGAAGCATT CTCGATTTGC AGGATCAAGT GTTCAGGAGC 2041 GTACTGATTG GACATTTCCA AAGCCTGCTC GTAGGTTGCA ACCGATAGGG TTGTAGAGTG 2101 TGCAATACAC TTGCGTACAA TTTCAACCCT TGGCAACTGC ACAGCTTGGT TGTGAACAGC 2161 ATCTTCAATT CTGGCAAGCT CCTTGTCTGT CATATCGACA GCCAACAGAA TCACCTGGGA 2221 ATCAATACCA TGTTCAGCTT GAGACAGAAG GTCTGAGGCA ACGAAATCTG GATCAGCGTA 2281 TTTATCAGCA ATAACTAGAA CTTCAGAAGG CCCAGCAGGC ATGTCAATAC TACACAGGGC 2341 TGATGTGTCA TTTTGAACCA TCATCTTGGC AGCAGTAACG AACTGGTTTC CTGGACCAAA 2401 TATTTTGTCA CACTTAGGAA CAGTTTCTGT TCCGTAAGCC ATAGCAGCTA CTGCCTGGGC 2461 GCCTCCTGCT AGCACGATAC ACTTAGCACC AACCTTGTGG GCAACGTAGA TGACTTCTGG 2521 GGTAAGGGTA CCATCCTTCT TAGGTGGAGA TGCAAAAACA ATTTCTTTGC AACCAGCAAC 2581 TTTGGCAGGA ACACCCAGCA TCAGGGAAGT GGAAGGCAGA ATTGCGGTTC CACCAGGAAT 2641 ATAGAGGCCA ACTTTCTCAA TAGGTCTTGC AAAACGAGAG CAGACTACAC CAGGGCAAGT 2701 CTCAACTTGC AACGTCTCCG TTAGTTGAGC TTCATGGAAT TTCCTGACGT TATCTATAGA 2761 GAGATCAATG GCTCTCTTAA CGTTATCTGG CAATTGCATA AGTTCCTCTG GGAAAGGAGC 2821 TTCTAACACA GGTGTCTTCA AAGCGACTCC ATCAAACTTG GCAGTTAGTT CTAAAAGGGC 2881 TTTGTCACCA TTTTGACGAA CATTGTCGAC AATTGGTTTG ACTAATTCCA TAATCTGTTC 2941 CGTTTTCTGG ATAGGACGAC GAAGGGCATC TTCAATTTCT TGTGAGGAGG CCTTAGAAAC 3001 GTCAATTTTG CACAATTCAA TACGACCTTC AGAAGGGACT TCTTTAGGTT TGGATTCTTC 3061 TTTAGGTTGT TCCTTGGTGT ATCCTGGCTT GGCATCTCCT TTCCTTCTAG TGACCTTTAG 3121 GGACTTCATA TCCAGGTTTC TCTCCACCTC GTCCAACGTC ACACCGTACT TGGCACATCT 3181 AACTAATGCA AAATAAAATA AGTCAGCACA TTCCCAGGCT ATATCTTCCT TGGATTTAGC 3241 TTCTGCAAGT TCATCAGCTT CCTCCCTAAT TTTAGCGTTC AACAAAACTT CGTCGTCAAA 3301 TAACCGTTTG GTATAAGAAC CTTCTGGAGC ATTGCTCTTA CGATCCCACA AGGTGGCTTC 3361 CATGGCTCTA AGACCCTTTG ATTGGCCAAA ACAGGAAGTG CGTTCCAAGT GACAGAAACC 3421 AACACCTGTT TGTTCAACCA CAAATTTCAA GCAGTCTCCA TCACAATCCA ATTCGATACC 3481 CAGCAACTTT TGAGTTGCTC CAGATGTAGC ACCTTTATAC CACAAACCGT GACGACGAGA 3541 TTGGTAGACT CCAGTTTGTG TCCTTATAGC CTCCGGAATA GACTTTTTGG ACGAGTACAC 3601 CAGGCCCAAC GAGTAATTAG AAGAGTCAGC CACCAAAGTA GTGAATAGAC CATCGGGGCG 3661 GTCAGTAGTC AAAGACGCCA ACAAAATTTC ACTGACAGGG AACTTTTTGA CATCTTCAGA 3721 AAGTTCGTAT TCAGTAGTCA ATTGCCGAGC ATCAATAATG GGGATTATAC CAGAAGCAAC 3781 AGTGGAAGTC ACATCTACCA ACTTTGCGGT CTCAGAAAAA GCATAAACAG TTCTACTACC 3841 GCCATTAGTG AAACTTTTCA AATCGCCCAG TGGAGAAGAA AAAGGCACAG CGATACTAGC 3901 ATTAGCGGGC AAGGATGCAA CTTTATCAAC CAGGGTCCTA TAGATAACCC TAGCGCCTGG 3961 GATCATCCTT TGGACAACTC TTTCTGCCAA ATCTAGGTCC AAAATCACTT CATTGATACC 4021 ATTATTGTAC AACTTGAGCA AGTTGTCGAT CAGCTCCTCA AATTGGTCCT CTGTAACGGA 4081 TGACTCAACT TGCACATTAA CTTGAAGCTC AGTCGATTGA GTGAACTTGA TCAGGTTGTG 4141 CAGCTGGTCA GCAGCATAGG GAAACACGGC TTTTCCTACC AAACTCAAGG AATTATCAAA 4201 CTCTGCAACA CTTGCGTATG CAGGTAGCAA GGGAAATGTC ATACTTGAAG TCGGACAGTG 4261 AGTGTAGTCT TGAGAAATTC TGAAGCCGTA TTTTTATTAT CAGTGAGTCA GTCATCAGGA 4321 GATCCTCTAC GCCGGACGCA TCGTGGCCGA CCTGCAGGGG GGGGGGGGGC GCTGAGGTCT 4381 GCCTCGTGAA GAAGGTGTTG CTGACTCATA CCAGGCCTGA ATCGCCCCAT CATCCAGCCA 4441 GAAAGTGAGG GAGCCACGGT TGATGAGAGC TTTGTTGTAG GTGGACCAGT TGGTGATTTT 4501 GAACTTTTGC TTTGCCACGG AACGGTCTGC GTTGTCGGGA AGATGCGTGA TCTGATCCTT 4561 CAACTCAGCA AAAGTTCGAT TTATTCAACA AAGCCGCCGT CCCGTCAAGT CAGCGTAATG 4621 CTCTGCCAGT GTTACAACCA ATTAACCAAT TCTGATTAGA AAAACTCATC GAGCATCAAA 4681 TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC 4741 TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG 4801 TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA 4861 AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAAAGC 4921 TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA 4981 CTCGCATCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA 5041 TCGCTGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC 5101 AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT 5161 TTCCCGGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG 5221 ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGTC TGACCATCTC ATCTGTAACA 5281 TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA 5341 TACAATCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA 5401 TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCCTCG AGCAAGACGT TTCCCGTTGA 5461 ATATGGCTCA TAACACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT TATTGTTCAT 5521 GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC AACGTGGCTT 5581 TCCCCCCCCC CCCTGCAGGT CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT 5641 ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT 5701 TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG 5761 CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC 5821 CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGAGTATCTA TGATTGGAAG TATGGGAATG 5881 GTGATACCCG CATTCTTCAG TGTCTTGAGG TCTCCTATCA GATTATGCCC AACTAAAGCA 5941 ACCGGAGGAG GAGATTTCAT GGTAAATTTC TCTGACTTTT GGTCATCAGT AGACTCGAAC 6001 TGTGAGACTA TCTCGGTTAT GACAGCAGAA ATGTCCTTCT TGGAGACAGT AAATGAAGTC 6061 CCACCAATAA AGAAATCCTT GTTATCAGGA ACAAACTTCT TGTTTCGAAC TTTTTCGGTG 6121 CCTTGAACTA TAAAATGTAG AGTGGATATG TCGGGTAGGA ATGGAGCGGG CAAATGCTTA 6181 CCTTCTGGAC CTTCAAGAGG TATGTAGGGT TTGTAGATAC TGATGCCAAC TTCAGTGACA 6241 ACGTTGCTAT TTCGTTCAAA CCATTCCGAA TCCAGAGAAA TCAAAGTTGT TTGTCTACTA 6301 TTGATCCAAG CCAGTGCGGT CTTGAAACTG ACAATAGTGT GCTCGTGTTT TGAGGTCATC 6361 TTTGTATGAA TAAATCTAGT CTTTGATCTA AATAATCTTG ACGAGCCAAG GCGATAAATA 6421 CCCAAATCTA AAACTCTTTT AAAACGTTAA AAGGACAAGT ATGTCTGCCT GTATTAAACC 6481 CCAAATCAGC TCGTAGTCTG ATCCTCATCA ACTTGAGGGG CACTATCTTG TTTTAGAGAA 6541 ATTTGCGGAG ATGCGATATC GAGAAAAAGG TACGCTGATT TTAAACGTGA AATTTATCTC 6601 AAGATCTCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 6661 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 6721 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG 6781 GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 6841 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC 6901 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 6961 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 7021 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 7081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 7141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 7201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 7261 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 7321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 7381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 7441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 7501 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 7561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 7621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 7681 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 7741 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 7801 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 7861 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 7921 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 7981 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 8041 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 8101 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGGCATCGT 8161 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 8221 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 8281 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 8341 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 8401 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA 8461 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 8521 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 8581 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 8641 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 8701 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 8761 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 8821 ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC 8881 GAGGCCCTTT CGTCTTCAAG AATTAATTCT CATGTTTGAC AGCTTATCAT CGATAAGCTG 8941 ACTCATGTTG GTATTGTGAA ATAGACGCAG ATCGGGAACA CTGAAAAATA ACAGTTATTA 9001 TTCG

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