| | Toledo |
| | H Ben-Bassat |
| Biosafety Level: | 1 |
| | frozen |
| Medium & Serum: | See Propagation |
| | suspension |
| | Homo sapiens deposited as human |
| | lymphoblast |
| | Organ: peripheral blood Disease: diffuse large cell lymphoma; non-Hodgkins B cell lymphoma Cell Type: B lymphocyte; |
| | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
|
| | Isolation date: 1990 |
| | CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289] |
| | D5S818:10,12 D7S820: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 Amelogenin: X CSF1PO: 12 D13S317:11 D16S539:9 |
| | adult |
| | female |
| | White |
| | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°C |
| | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| | 24 to 30 hrs |
| | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020 |
| | 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
