Description: CML, Human, mAb CML26

The monoclonal antibody CML26 recognizes human CML (carboxymethyl-lysine). CML is known to be formed from the oxidation of both carbohydrates and lipids. This makes CML- a biomarker of general oxidative stress. Carboxymethyl-lysine (CML) is a well-characterized glycoxidation product that accumulates in tissues with age, and its rate of accumulation is accelerated in diabetes. Glycoxidation products are a subset of advanced glycation endproducts (AGEs) that are formed by the nonenzymatic glycation and subsequent irreversible oxidation of proteins. Oxidative stress and protein modification have been implicated in the pathogenesis of the chronic complications of diabetes, including nephropathy and atherosclerosis. The accumulation of CML in long-lived tissue such as skin collagen reflects oxidative stress over an extended period of the life-span, and has been shown to be greater in patients with diabetic complications than those without complications.

Specifications:

Catalog number Product type Quantity Formulation Application Application Notes Use Immunogen Isotype Species Positive Control References Storage and stability Precautions Disease

HM5013

Monoclonal antibodies

100 µg

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide

Frozen sections, Immuno assays, Immuno fluorescence, Paraffin sections, Western blot

P: fixation in 4% formalin; cardiac tissue sections (4 mm) deparaffinised for 10 min in xylene at room temperature, dehydrated by decreasing ethanol. Sections stained with haematoxylin and eosin. Blocking endogenous peroxidase activity with 0.3% hydrogen peroxide in methanol for 30 min. No heating to prevent artificial induction of CML. (Ref 1)IF: After fixation in 2% phosphate-buffered glutaraldehyde solution the heart tissue was post-fixed in 1% osmium tetroxide. The tissue was dehydrated through a graded series of ethanol. 0.53.0- mm-thick sections were cut with a glass knife.(Ref 1)

For immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

CML-KLH

Mouse IgG1

Human

Intramyocardial arteries

1. Bruynzeel, AM. Anti-inflammatory agents and monoHER protect against DOX-inducedcardiotoxicity and accumulation of CML in mice. B J of Cancer 2007: 96, 9372. Ciapaite J. Functioning of oxidative phosphorylation in liver mitochondria of high-fat diet fed rats.Biochim Biophys Acta. 2007, 1772:307.3. Baidoshvili A. N(varepsilon)-(carboxymethyl)lysine depositions in intramyocardial blood vessels inhuman and rat acute myocardial infarction: a predictor or reflection of infarction?ArteriosclerThromb Vasc Biol. 2006, 26:2497.4. Lieuw-a-Fa ML. Interaction of Nepsilon(carboxymethyl)lysine- and methylglyoxal-modifiedalbumin with endothelial cells and macrophages. Splice variants of RAGE may limit theresponsiveness of human endothelial cells to AGEs. Thromb Haemost. 2006, 95:320.5. van Heijst JW. Argpyrimidine-modified Heat shock protein 27 in human non-small cell lungcancer: a possible mechanism for evasion of apoptosis.Cancer Lett. 2006, 241:309.6. Sommeijer DW. More fibrosis and thrombotic omplications but similar expression patterns ofmarkers for coagulation and inflammation in symptomatic plaques from DM2 patients. JHistochem Cytochem. 2004 , 52:11417. Schalkwijk CG. Increased accumulation of the glycoxidation product Nepsilon-(carboxymethyl)lysine in hearts of diabetic patients: generation and characterisation of a monoclonal anti-CMLantibody. Biochim Biophys Acta. 2004, 221636:28. Baidoshvili A. N(omega)-(carboxymethyl)lysine depositions in human aortic heart valves:similarities with atherosclerotic blood vessels. Atherosclerosis. 2004, 174:287.

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Cardiology and metabolism

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