Description: S100A7, Human, mAb 9F3

Antibody 9F3 recognizes human S100A7 (Psoriasin). S100A7 is a 11.4kDa member of the S100 gene family, which are EF-hand Ca 2+-binding proteins. They are involved in a broad range of intra- and extracellular processes like regulation of calcium homeostasis, cell proliferation, differentiation, apoptosis, immune responses, antimicrobial activity, chemotaxis and inflammation. S100 proteins have a fundamental structural unit which is a highly integrated dimer, some as homodimer and others as heterodimer. S100A7 functions as a homodimer and binds two Zn2+ ions. These ions stabilize the dimer and mediate the function of S100A7 during infection. Extracellular S100A7 functions as a DAMP during infection. S100A7 limits bacterial infection through metal limitation. Furthermore, S100A7 interacts with RAGE which drives an NF-κB mediated proinflammatory response and recruitment of neutrophils.S100A7 was first discovered as a protein abundantly expressed in psoriatic keratinocytes. Possibly, S100A7 adheres directly to, and reduces survival of, pathogens found on the epidermis and acts as a physical barrier. S100A7 also seems to be involved with breast cancer and squamous cell carcinoma. In vitro it was found to confer a more aggressive behavior.

Specifications:

Catalog number Product type Quantity Formulation Application Application Notes Use Immunogen Isotype Species Cross reactivity Alias Positive Control Negative Control Storage and stability Precautions Disease

HM2328

Monoclonal antibodies

100 µg

1 ml (100 μg/ml) 0.2 μm protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.

Functional studies, Immuno assays, Immuno fluorescence, Paraffin sections, Western blot

FS: Monoclonal antibody 9F3 block tumor cell proliferation induced by S100A7 in fibrosarcoma cell line HT1080.W: A reduced sample treatment and SDS-Page was used.IF: For Immuno fluorescence a concentration of 5μg/ml of HM2328 was used.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Full length recombinant human S100A7

Mouse IgG1

Human

Canine - Yes, Mouse - No, Rabbit - Yes, Rat - Yes, Sheep - Yes

Protein S100-A7, Psoriasin, S100 calcium-binding protein A7Gene name: S100A7, PSOR1, S100A7C

A431 tumors (Paraffin embedded sections)

MiaPACA-2 tumors (Paraffin embedded sections)

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Autoimmunity, Tumor immunology

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